Transcriptome profiling reveals genes involved in spine development during CsTTG1-regulated pathway in cucumber (Cucumis sativus L.).

The cucumber (Cucumis sativus L.), a type of fleshy fruit, is covered with spines (multicellular trichomes), which have a crucial impact on the economic value of the crop. Previous studies have found that CsTTG1 plays important roles in the initiation and further differentiation of cucumber spines, but how spine formation is regulated at the molecular level by CsTTG1 remains poorly understood. In this study, we characterized a cucumber 35S:CsTTG1 transgenic T2 line, OE-2, which bears relatively large and long spines compared with the small and short spines of the wild type (WT). Phenotypic measurements and histological analyses revealed that this phenotypic change was attributed to significant increases in cell number and size. Comparison of ovary epidermis transcriptomes between OE-2 and WT by DGE (Digital Gene Expression) analysis identified 1241 differentially expressed genes, among which 712 genes were dramatically upregulated and 529 downregulated in the ovary epidermis of OE-2. XTH23 and Cyclin family genes were significantly activated in OE-2, and transcription factors (TFs) were found to participate in spine size regulation in OE-2. Further analyses confirmed that GA was implicated in the regulation of fruit spine development in cucumber. Thus, our study provides a foundation for dissecting the molecular regulatory networks of fruit spine control in cucumber.