| Literature DB >> 31925742 |
José Cavalcante Souza Vieira1,2, Grasieli de Oliveira3, Camila Pereira Braga4, Mileni da Silva Fernandes5, Paula Martin de Moraes6, Marília Afonso Rabelo Buzalaf5, Lincoln Carlos Silva de Oliveira7, Pedro de Magalhães Padilha3.
Abstract
Recent studies have demonstrated the association of mercury (Hg) with some fish proteins, milk, and hair from individuals exposed to the element in the Amazon. However, few studies involve identifying biomarkers of mercury exposure. Therefore, the present study aimed to identify potential biomarkers of Hg exposure in fish. For this, the muscular tissues of two species of fish (Prochilodus lineatus and Mylossoma duriventre) that feed the Amazonian human population were analyzed. Through the analyses obtained by graphite furnace atomic absorption spectrometry (GFAAS), it was possible to identify four protein SPOTS where mercury was present. These SPOTS, identified by mass spectrometry (ESI-MS/MS), included parvalbumin and ubiquitin-40S ribosomal protein S27a, and these being metalloproteins with biomarker characteristics. In addition, the results show the intense Hg/protein ratio observed in the two proteins, which makes metalloproteins strong candidates for biomarkers of mercury exposure. Graphical Abstract.Entities:
Keywords: Biomarkers; Fish; Mercury in the Amazon; Mercury-bound proteins; Metalloproteins; Proteomics
Mesh：
Substances：
Year: 2020 PMID： 31925742 DOI： 10.1007/s12011-020-02026-w
Source DB: PubMed Journal: Biol Trace Elem Res ISSN： 0163-4984 Impact factor: 3.738