F Camacho-Alonso1, C Martínez-Ortiz2, L Plazas-Buendía2, A M Mercado-Díaz2, C Vilaplana-Vivo3, J A Navarro4, A J Buendía4, J J Merino5, Y Martínez-Beneyto6. 1. Department of Oral Surgery, University of Murcia, Murcia, Spain. fcamacho@um.es. 2. In private dental practice, Murcia, Spain. 3. Department of Oral Surgery, University of Murcia, Murcia, Spain. 4. Department of Histology and Pathological Anatomy, University of Murcia, Murcia, Spain. 5. Department of Biochemistry and Molecular Biology, Complutense University of Madrid, Madrid, Spain. 6. Department of Preventive and Community Dentistry, University of Murcia, Murcia, Spain.
Abstract
OBJECTIVE: The objective is to compare new bone formation in critical defects in healthy, diabetic, and osteoporotic rats filled with hydroxyapatite (HA) alone and HA combined with simvastatin (SV). MATERIALS AND METHODS: A total of 48 adult female Sprague-Dawley rats were randomized into three groups (n = 16 per group): Group, 1 healthy; Group 2, diabetics; and Group 3, osteoporotics. Streptozotocin was used to induce type 1 diabetes in Group 2, while bilateral ovariectomy was used to induce osteoporosis in Group 3. The central portion of the rat mandibular symphysis was used as a physiological critical bone defect. In each group, eight defects were filled with HA alone and eight with HA combined with SV. The animals were sacrificed at 4 and 8 weeks, and the mandibles were processed for micro-computed tomography to analyze radiological union and bone mineral density (BMD); histological analysis of the bone union; and immunohistochemical analysis, which included immunoreactivity of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2). RESULTS: In all groups (healthy, diabetics, and osteoporotics), the defects filled with HA + SV presented greater radiological bone union, BMD, histological bone union, and more VEGF and BMP-2 positivity, in comparison with bone defects treated with HA alone. CONCLUSIONS: Combined application of HA and SV improves bone regeneration in mandibular critical bone defects compared with application of HA alone in healthy, diabetic, and osteoporotic rats. CLINICAL RELEVANCE: This study might help to patients with osteoporosis or uncontrolled diabetes type 1, but future studies should be done.
OBJECTIVE: The objective is to compare new bone formation in critical defects in healthy, diabetic, and osteoporoticrats filled with hydroxyapatite (HA) alone and HA combined with simvastatin (SV). MATERIALS AND METHODS: A total of 48 adult female Sprague-Dawley rats were randomized into three groups (n = 16 per group): Group, 1 healthy; Group 2, diabetics; and Group 3, osteoporotics. Streptozotocin was used to induce type 1 diabetes in Group 2, while bilateral ovariectomy was used to induce osteoporosis in Group 3. The central portion of the rat mandibular symphysis was used as a physiological critical bone defect. In each group, eight defects were filled with HA alone and eight with HA combined with SV. The animals were sacrificed at 4 and 8 weeks, and the mandibles were processed for micro-computed tomography to analyze radiological union and bone mineral density (BMD); histological analysis of the bone union; and immunohistochemical analysis, which included immunoreactivity of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2). RESULTS: In all groups (healthy, diabetics, and osteoporotics), the defects filled with HA + SV presented greater radiological bone union, BMD, histological bone union, and more VEGF and BMP-2 positivity, in comparison with bone defects treated with HA alone. CONCLUSIONS: Combined application of HA and SV improves bone regeneration in mandibular critical bone defects compared with application of HA alone in healthy, diabetic, and osteoporoticrats. CLINICAL RELEVANCE: This study might help to patients with osteoporosis or uncontrolled diabetes type 1, but future studies should be done.
Entities:
Keywords:
Bone regeneration; Critical bone defect; Diabetes; Hydroxyapatite; Mandibular symphysis; Osteoporosis; Rats; Simvastatin
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Authors: Şeref Ezirganlı; Hakkı O Kazancıoğlu; Ahmet Mihmanlı; Mehmet Ş Aydın; Rasul Sharifov; Alpay Alkan Journal: Clin Oral Implants Res Date: 2013-04-21 Impact factor: 5.977
Authors: Fabio Camacho-Alonso; M R Tudela-Mulero; J A Navarro; A J Buendía; A M Mercado-Díaz Journal: Clin Oral Investig Date: 2022-05-07 Impact factor: 3.606