Literature DB >> 3192529

Crystallization of actophorin, an actin filament-severing protein from Acanthamoeba.

K A Magnus1, S K Maciver, T D Pollard.   

Abstract

Actophorin is an actin monomer-binding and actin filament-severing protein from Acanthamoeba castellanii. It crystallizes out of polyethylene glycol in a form suitable for high resolution x-ray analysis. The crystals are orthorhombic and have the symmetry of the space group P2(1)2(1)2(1) with lattice constants a = 39.8 +/- 0.5, b = 47.3 +/- 0.6, and c = 69.9 +/- 1.6 A. They diffract to a resolution of at least 2.8 A, and the asymmetric unit contains one actophorin monomer of Mr 15,000.

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Year:  1988        PMID： 3192529

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  5 in total

1.  Regulation of the actin cycle in vivo by actin filament severing.

Authors:  J L McGrath; E A Osborn; Y S Tardy; C F Dewey; J H Hartwig
Journal:  Proc Natl Acad Sci U S A       Date:  2000-06-06       Impact factor: 11.205

Review 2.  The ADF/cofilin proteins: stimulus-responsive modulators of actin dynamics.

Authors:  A Moon; D G Drubin
Journal:  Mol Biol Cell       Date:  1995-11       Impact factor: 4.138

3.  Improved resolution crystal structure of Acanthamoeba actophorin reveals structural plasticity not induced by microgravity.

Authors:  Stephen Quirk; Raquel L Lieberman
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2021-11-11       Impact factor: 1.056

4.  Structure and activity of a thermally stable mutant of Acanthamoeba actophorin.

Authors:  Stephen Quirk; Raquel L Lieberman
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2022-03-28       Impact factor: 1.056

5.  Characterization of actin filament severing by actophorin from Acanthamoeba castellanii.

Authors:  S K Maciver; H G Zot; T D Pollard
Journal:  J Cell Biol       Date:  1991-12       Impact factor: 10.539
  5 in total

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