Early fulminant BK polyomavirus-associated nephropathy in two kidney transplant patients with low neutralizing antibody titers receiving allografts from the same donor.

BACKGROUND: BK Polyomavirus (BKPyV) causes premature graft failure in 1 to 15% of kidney transplant (KT) recipients. High-level BKPyV-viruria and BKPyV-DNAemia precede polyomavirus-associated nephropathy (PyVAN), and guide clinical management decisions. In most cases, BKPyV appears to come from the donor kidney, but data from biopsy-proven PyVAN cases are lacking. Here, we report the early fulminant course of biopsy-proven PyVAN in two male KT recipients in their sixties, receiving kidneys from the same deceased male donor. CASE PRESENTATIONS: Both recipients received intravenous basiliximab induction, and maintenance therapy consisting of tacrolimus (trough levels 3-7 ng/mL from time of engraftment), mycophenolate mofetil 750 mg bid, and prednisolone. At 4 weeks post-transplant, renal function was satisfactory with serum creatinine concentrations of 106 and 72 μmol/L in recipient #1 and recipient #2, respectively. Plasma BKPyV-DNAemia was first investigated at 5 and 8 weeks post-transplant being 8.58 × 104 and 1.12 × 106 copies/mL in recipient #1 and recipient #2, respectively. Renal function declined and biopsy-proven PyVAN was diagnosed in both recipients at 12 weeks post-transplant. Mycophenolate mofetil levels were reduced from 750 mg to 250 mg bid while tacrolimus levels were kept below 5 ng/mL. Recipient #2 cleared BKPyV-DNAemia at 5.5 months post-transplant, while recipient #1 had persistent BKPyV-DNAemia of 1.07 × 105 copies/mL at the last follow-up 52 weeks post-transplant. DNA sequencing of viral DNA from early plasma samples revealed apparently identical viruses in both recipients, belonging to genotype Ib-2 with archetype non-coding control region. Retrospective serological work-up, demonstrated that the donor had high BKPyV-IgG-virus-like particle ELISA activity and a high BKPyV-genotype I neutralizing antibody titer, whereas both KT recipients only had low neutralizing antibody titers pre-transplantation. By 20 weeks post-transplant, the neutralizing antibody titer had increased by > 1000-fold in both recipients, but only recipient #2 cleared BKPyV-DNAemia.
CONCLUSIONS: Low titers of genotype-specific neutralizing antibodies in recipients pre-transplant, may identify patients at high risk for early fulminant donor-derived BKPyV-DNAemia and PyVAN, but development of high neutralizing antibody titers may not be sufficient for clearance.

Background

BK Polyomavirus (BKPyV) infects about 90% of the world’s population [3, 14]. After primary infection, which usually goes unnoticed, the virus persists quietly in the epithelial cells of the reno-urinary tract. Asymptomatic low-level virus shedding in the urine has been detected in healthy immunocompetent blood donors indicating immune escape of BKPyV [6, 17]. In kidney transplant (KT) recipients, where the immune system is suppressed by immunosuppressive drugs in order to avoid rejection, the prevalence of viruria increases to more than 60%, and about half of these viruric patients develop high-level BKPyV viruria defined as > 7 log10 copies (c) per mL and shed decoy cells. About 2 to 6 weeks later, approximately half of these patients progress to BKPyV-DNAemia and biopsy-proven polyomavirus-associated nephropathy (PyVAN). The disease is characterized by persisting high-level BKPyV replication in the tubular epithelial cells of the kidney allograft, causing cytopathic loss. The disruption of the epithelial cell monolayer leads to leakage of virus and viral DNA into the tissue and blood stream i.e. BKPyV DNAemia, and is followed by a local inflammation [4, 12, 22]. In addition, high-level BKPyV replication in the multilayered epithelium of the renal pelvis and the bladder, contribute to the viruria. As antiviral drugs for treatment of PyVAN are lacking, the mainstay therapy is a stepwise reduction of immunosuppression [13]. Without this intervention, more than 90% of affected KT recipients will show a declining kidney allograft function and experience premature graft loss.

BKPyV has a circular double-stranded DNA genome of about 5 kb. The genetic heterogeneity in the VP1 gene encoding the major capsid protein Vp1, can be used to divide BKPyV into four sero−/genotypes (I, II, III, IV) [15], two of which can be further divided into subtypes (Ia, Ib-1, Ib-2, Ic, IVa-I, IVa-2, IVb-1, IVb-2, IVc-I and IVc-2) [38]. Another genome sequence used to characterize the virus is the non-coding control region (NCCR) which comprises the origin of viral genome replication and promoter/enhancer functions. In urine from immunocompetent individuals, BKPyV typically has an archetype NCCR architecture that has been arbitrarily divided into five sequence blocks denoted O142 - P68 - Q39 - R63 - S63, where the subscript number indicates the number of base pairs. Early in the course of PyVAN, BKPyV strains with an archetype NCCR are found in urine and plasma. Presumably due to the lack of a functional T-cell immunity, these strains are gradually replaced by faster replicating strains with a rearranged NCCRs showing an upregulated expression of the early regulatory protein large T-antigen (LTag) [9, 23, 24].

Since PyVAN preferentially affects KT recipients, PyVAN has been suggested to arise mainly due to donor-derived infection [2]. This concept is supported by the detection of identical BKPyV-genotypes and/or strains in the donor urine pre-transplant and in the recipients urine and/or plasma post-transplant [2, 29, 30, 35, 37]. Moreover, a study of 21,575 recipient pairs receiving kidneys from the same donor supported this concept, as BKPyV replication was reported in twice as many recipient pairs (n = 174) than expected by chance [32]. However, data from recipient pairs with biopsy-proven nephropathy are lacking.

Here, we describe the course of two KT patients developing early fulminant biopsy-proven PyVAN after receiving their allografts from the same deceased donor. Retrospective sequencing of the BKPyV genome indicated that PyVAN developed as a result of transmission of donor-derived BKPyV. Detailed serological studies identified low neutralizing antibody titers in both recipients pre-transplant as a potential marker of low antiviral immune control and increased risk for BKPyV-DNAemia and PyVAN. Although both recipients developed a more than 1000-fold increase in neutralizing antibody (NAb) titers, only one recipient cleared BKPyV-DNAemia. The potential role of viral and immune markers for screening, monitoring and follow-up is discussed.

Case presentation

Deceased donor

The donor was a 62-year old male who died from a subarachnoid hemorrhage. He was IgG-seropositive for cytomegalovirus (CMV) and had blood group A. Retrospective investigation of his plasma using three different serological methods (reviewed in [17]) demonstrated high-levels of BKPyV neutralizing antibodies. In more detail, using a neutralization assay, a more than 50% inhibition of genotype I-pseudovirus infectivity was obtained when a 640-fold plasma dilution was used, which corresponds to a NAb titer of 640 half maximal inhibitory concentration (IC50). The method used was modified from a protocol by Pastrana and colleagues [25] by using a pseudovirus containing pEGFP-N1 instead of phGluc. As a consequence, infectivity was measured as fluorescent intensity instead of luciferase activity. The hemagglutination inhibition assay (HIA) [21], measured a HIA-titer of 320. Finally, the BKPyV-IgG specific enzyme-linked immunosorbent assay (ELISA) using Vp1-derived virus-like particles [16], gave a normalized optical density (nOD) of 2.329 for a plasma dilution of 400, but no IgM was detectable. Moreover, using a validated quantitative real-time PCR assay [5], no BKPyV-DNA was detectable in the donor plasma. Besides, immunohistochemistry of the baseline kidney biopsy using a commercial antibody directed against SV40 LTag (Pab416, Merck) but known to cross-react with BKPyV LTag, was negative.

Case 1

Recipient #1 was a 68 year old male with end-stage kidney disease due to granulomatosis with polyangiitis requiring hemodialysis for the last two years. At the time of transplantation, he had a serum creatinine (s-Cr) of 457 μmol/L (Fig. 1a). Human leukocyte antigen (HLA) typing showed one HLA-A, one HLA-B and one HLA-DR mismatches. His blood group was the same as for the donor and he was seropositive for CMV-IgG, thus yielding an intermediate risk for CMV (D+/R+). No known panel reactive antibody (PRA) or donor specific antibodies (DSA) were detected i.e. the recipient had a standard immunologic risk. He received standard immunosuppressive therapy; intravenous (i.v.) basiliximab induction, prednisolone, tacrolimus (trough levels 3–7 ng/mL from time of engraftment), and mycophenolate mofetil (MMF) 750 mg bid. Four days post-transplant, his s-Cr level was 302 μmol/L, decreasing to 106 μmol/L by 4 weeks post-transplant (Fig. 1a). One week later (5 weeks post-transplant), his plasma was, for the first time, analyzed for BKPyV-DNAemia and 8.58 × 104 c/mL were detected (Fig. 1b), giving him the diagnosis presumptive PyVAN.

Fig. 1

Treatment and Clinical Course of Recipient #1. The X-axis is labeled with the week after transplantation when the sample was taken. Arrows indicate biopsies. Y-axis: a Serum creatinine concentration in μmol/L. b BKPyV-DNA genome levels in plasma (red triangles) and in urine (yellow bar) in log10 c/mL. Retrospectively tested plasma samples (black triangles). c BKPyV-antibody IgG (green line) and IgM (blue line) shown as nOD at the left Y-axis; HIA-titer (orange bar) at the right Y-axis

At 12 weeks post-transplant, his BKPyV plasma load had increased by 3 orders of magnitude to 1.66 × 107 c/mL (Fig. 1b), and the s-Cr level had increased to 139 μmol/L (Fig. 1a). Therefore, an allograft biopsy was taken. The biopsy showed no interstitial inflammation, no intimal arteritis, and no rejection, but mild tubulitis (Banff score of i0t1v0, C4d negative) (Fig. 2a). In addition, positive immunostaining for LTag was observed in some tubular epithelial cells (Fig. 2b), establishing the diagnosis of proven-PyVAN (Stage-B1) [13]. Therefore, MMF was reduced from 750 mg to 250 mg bid while tacrolimus treatment with already low trough levels was left unchanged.

Fig. 2

Histological analysis of a renal allograft biopsy from recipient #1 at 12 weeks post-transplant. a HES (hematoxylin, eosin and saffron) stained section. Original magnification 200x, scale bar =100 μm. b Immunohistochemistry staining of the same biopsy as in a), viral LTag expression (brown colour) in tubular epithelial cells using the cross-reacting monoclonal anti-SV40 LTag antibody Pab416 (Merck). Original magnification 400x, scale bar =50 μm

Seven weeks later (19 weeks post-transplant), the plasma BKPyV load had decreased to 6.35 × 105 c/mL (Fig. 1b). Subsequently, the patient was seen in his local hospital, where the s-Cr was reported as stable and plasma BKPyV-DNAemia was not examined. At the planned one-year post-transplant surveillance control, the s-Cr was stable at 135 μmol/L, the plasma BKPyV-load was still 1.07 × 105 c/mL (Fig. 1b), and the urine BKPyV-load was high with 6.71 × 109 c/mL (Fig. 1b). The protocol biopsy showed no signs of inflammation or rejection (Banff score of i0t0v0, C4d negative) and no detectable LTag staining (results not shown) (Fig. 1a).

Retrospective testing of plasma samples taken the first four weeks post-transplant did not detect BKPyV-DNAemia (Fig. 1b, black triangles). Nevertheless, BKPyV-ELISA revealed that recipient #1 was IgG seropositive (0.442 nOD) and IgM seronegative pre-transplantation. Of note, the pre-transplant HIA-titer was 80 (Fig. 1c), and the BKPyV-genotype I NAb-titer was only 10 IC50.

During the first 5 weeks post-transplant, a slow but continuous increase of the ELISA-IgG activity was found. Then a more rapid increase was seen with a peak value of nOD 2.646 at 19 weeks post-transplant (the last measured time point). During this last phase, the BKPyV-IgM became detectable and peaked at 15 weeks post-transplant (Fig. 1c), indicating a significant immune response to BKPyV-antigens.

At 19 weeks post-transplant, the ELISA IgG and the HIA-titer had increased by six-fold and 256-fold, whereas the NAb-titer had increased by > 1000-fold to > 10,240 IC90 i.e. the plasma inhibited more than 90% of the infectious activity at 1:10240 dilution.

Case 2

Recipient #2 was a 62 year old male with autosomal polycystic kidney disease. He had a s-Cr of 401 μmol/L pre-transplantation (Fig. 3a). HLA typing showed one HLA-A, two HLA-B, and one HLA-DR mismatches. The recipient’s blood group was the same as the donor’s and he had an intermediate risk for CMV (D+/R+). No known PRA or DSA were detected and he received the same immunosuppressive therapy as recipient #1. His baseline renal function was good with serum s-Cr levels decreasing from 112 μmol/L at 4 days post-transplant to 72 μmol/L at 5 weeks post-transplant (Fig. 3a).

Fig. 3

Treatment and Clinical Course of Recipient #2. The X-axis is labeled with the week after transplantation when the sample was taken. Arrows indicate biopsies. Y-axis: a Serum creatinine concentration in μmol/L. b BKPyV-DNA genome levels in plasma (red triangles) and in urine (yellow bar) in log10 c/mL. Retrospectively tested plasma samples (black triangles). c BKPyV-antibody IgG (green line) and IgM (blue line) shown as nOD at the left Y-axis; HIA-titer (orange bar) at the right Y-axis.

However, at 6 weeks post-transplant, the s-Cr suddenly increased to 124 μmol/L (Fig. 3a). At 8 weeks post-transplant, the plasma was for the first time analyzed for BKPyV-DNAemia and 1.12 × 106 c/mL was detected (Fig. 3b), giving the diagnosis of presumptive PyVAN. An allograft biopsy was taken, but HES staining showed no signs of inflammation or rejection (Banff score i0t0v0, C4d negative) and immunohistochemical staining was negative for LTag (data not shown). The plasma BKPyV-DNAemia persisted at levels > 6 log10 c/mL (Fig. 3b), and at 12 weeks post-transplant a second allograft biopsy was taken. This time the biopsy showed focal interstitial inflammation and severe tubulitis (Banff score i2t3v0, C4d negative) (Fig. 4a). In addition, immunostaining revealed LTag-positive epithelial cells (Fig. 4b) giving the diagnosis of biopsy-proven PyVAN (stage B1). MMF was reduced from 750 mg to 250 mg bid, while tacrolimus treatment was left unchanged (trough levels ng/mL). At 20 weeks post-transplant, the plasma BKPyV-DNA load had declined to 3.56 × 104 c/mL and at 29 weeks post-transplant, BKPyV-DNAemia was no longer detectable (Fig. 3b). Concurrently the s-Cr was 155 μmol/L (Fig. 3a). One year post-transplantation, the s-Cr had declined to 130 μmol/L (Fig. 3a), plasma was still negative for BKPyV-DNAemia (Fig. 3b) while urine was positive with a low BKPyV load of 1.6 × 105 c/mL (Fig. 3b). The protocol biopsy showed limited inflammation and mild tubulitis (Banff score of i1t1v0, corresponding to Banff borderline for rejection, C4d negative) and negative LTag staining (results not shown).

Fig. 4

Histological analysis of a renal allograft biopsy from recipient #2 at 12 weeks post-transplant. a HES (hematoxylin, eosin and saffron) stained section showing inflammation (arrows). Original magnification 200x, scale bar = 100 μm. b Immunohistochemistry staining of the same biopsy as in a), showing LTag expression (brown colour) in tubular epithelial cells when the monoclonal anti–SV40 LTag antibody Pab416 (Merck) is used. Original magnification 400x, scale bar = 50 μm

Retrospective testing of plasma BKPyV-DNAemia revealed 2.59 × 103 c/mL in plasma already at 4 weeks post-transplant (Fig. 3b, black triangles). Besides, BKPyV-ELISA demonstrated that recipient #2 was IgG seropositive (nOD of 0.191) and IgM seronegative pre-transplantation. As for recipient #1, the pre-transplant HIA-titer was 80 (Fig. 3c), and the BKPyV-genotype I NAb-titer was only 10 IC50. During the first 7 weeks post-transplant, a slow but continuous increase of the BKPyV-IgG titer was found. Then a more rapid increase was seen until the IgG titer plateaued from 13 weeks post-transplant with a maximum nOD of 3.017 at 17 weeks post-transplantation. From 4 weeks post-transplant the BKPyV-IgM became positive and from 11 weeks post-transplant the HIA-titer peaked with 10,240 (Fig. 3c). At 20 weeks post-transplant, the ELISA IgG and the HIA-titer had increased by 16-fold and 128-fold, whereas the NAb-titer had increased by > 1000-fold to > 10,240 IC90.

Genetic analysis of BKPyV DNA in plasma and urine samples from both patients

In order to investigate the genotype and strain of BKPyV in plasma and urine samples, two nested PCRs were used to amplify a 330 base pair fragment of the VP1 gene and the complete NCCR [19]. The sequence results from both early plasma samples and urine samples from both recipients revealed virus of genotype Ib-2 having identical archetype NCCR. These results suggest that both recipients were infected with an identical BKPyV strain. However, one year post-transplant plasma sample of recipient #1, also contained strains with NCCR rearrangements, including one strain denoted RH-20 (GenBank Accession number MN627732), having a 60 bp deletion in the Q- and R-block removing the Sp1–4 transcription factor binding site [1].

Discussion and conclusions

In this study we report the parallel onset of early fulminant biopsy-proven PyVAN in two KT patients having received one kidney each from the same deceased donor. DNA sequencing of BKPyV DNA amplified from early plasma and urine samples, revealed an apparently identical virus of genotype Ib-2 with archetype NCCRs, in both recipients. This together with the clinical course, supports the notion of donor kidney transmission of BKPyV. Both recipients shared several previously reported risk factors for PyVAN [13] such as being males in their sixties and receiving treatment with tacrolimus-mycophenolic acid, whereas other risk factors such as lymphocyte-depleting induction or acute rejection episodes treated with steroid pulses were not present.

Our retrospective analyses revealed that the donor and both recipients were BKPyV-IgG seropositive before transplantation, but significantly differed in their NAb-titers for the replicating BKPyV genotype, which was almost 100-fold higher in the donor than in the recipients. These observations in the recipients are in line with a recent study by Solis and colleagues [31]. They reported that low NAb-titers against the donor BKPyV genotype, here defined as less than 4 log10 IC50, was associated with an increased risk of BKPyV-DNAemia and PyVAN. Despite this striking similarity, the titers may not be directly comparable, since they used a slightly different protocol.

Remarkably, the BKPyV-genotype I NAb-titers increased in both of our patients by more than 1000-fold to 10,240 IC90, thereby, reaching titers associated with clearance of BKPyV DNAemia [31]. Indeed, following MMF reduction, BKPyV-DNAemia declined in recipient #2, and cleared with 3 months. In contrast, recipient #1 had persistent BKPyV-DNAemia levels above 105 c/mL and high-level viruria detectable at one year post-transplant. Moreover, as previously reported [9, 23], the archetype NCCR of the BKPyV genome was now replaced by a rearranged NCCR in line with on-going intra-patient evolution and insufficient antiviral immunity. In particular, CD8 T cells directed against immunodominant 9mer epitopes derived from the viral early protein LTag has been implicated in clearance of BKPyV-DNAemia [17, 20]. Such immunodominant epitopes are presented by HLA-B51 which alone or in combination with HLA-B7 and -B8 has been associated with a lower risk of BKPyV DNAemia [34, 36]. Both recipients lacked these HLA types, except recipient #2 having HLA-B7. Possibly, lack of these HLA-types contributed to the rapid onset and protracted course of PyVAN.

Although we cannot exclude a synergizing role of neutralizing antibodies in the control of BKPyV replication in the affected tubulus of a given nephron, it remains unclear how sufficient antibodies can prevent the well documented cell to cell spread in the nephron.

We noted that the donor was in an age group that is characterized by low titers of BKPyV-specific IgG [10, 18, 28]. In our comprehensive serological assessment using three different assays, however, the donor had high BKPyV-IgG ELISA activity (2.329 nOD), a high HIA-titer (320) as well as a high NAb-titer (> 640 IC50). These results suggest that the immune system of the donor had been exposed to BKPyV recently. Considering the donor’s age and the undetectable BKPyV-IgM, this exposure was probably not due to a primary infection, but rather a recent reactivation leading to increased viral loads in his kidneys. Although no pre-transplant viruria samples from the donor were available, the high neutralizing activity against BKPyV of genotype I and the fact that BKPyV genotypes are serologically distinct [26], argues for transmission of genotype I, which also was found in the recipients.

Our parallel kidney transplant case studies from a single donor are also notable for further specific details. Unlike in the donor, the BKPyV-specific antibodies measured by ELISA and by the neutralization assay were discordant in both recipients with respect to the level at the time of transplantation, being higher in the former assay, but nearly undetectable in the latter. This suggest that the ELISA is more sensitive, but less specific for a given BKPyV genotype than the neutralization assay. This may also explain the lack of association of recipient ELISA antibody levels with BKPyV-DNAemia seen in a recent study of living donor-recipient pairs [11]. Moreover, from three weeks post-transplant, the ELISA titers started to increase suggesting a CD4-T cell help independent memory B-cell response to viral antigen exposure, for example resulting from donor virus replication in both kidney allografts directly after transplantation. This interpretation is supported by the fact that the antibody levels increased in parallel with increasing BKPyV-DNAemia before immunosuppression was reduced.

Another aspect is the observation that the first biopsy of recipient #2 was negative for BKPyV-LTag expression although BKPyV-DNAemia was higher than > 106 c/ml. Only a second biopsy taken 4 weeks later confirmed proven PyVAN. This suggests that the biopsy must have missed the typically focally arranged LTag positive epithelial cells, which has been previously documented in a study involving 41 KT recipients with persisting high-level BKPyV-DNAemia [4]. In this study multiple biopsy cores were taken at the same time, and discordant LTag-positive and LTag-negative biopsy cores were found in more than 30% of the cases. The focal nature of PyVAN may also explain why the baseline biopsy at transplantation and the protocol biopsy taken one year post-transplant of recipient #1 were negative. Cases of allograft nephrectomy have clearly demonstrated that BKPyV-DNAemia is derived directly from the renal allograft [7, 8] and BKPyV-DNAemia is now considered a direct biological marker of PyVAN [13]. Importantly this has been implemented in the recently updated guidelines on BKPyV in solid organ transplantation [13]. A renal allograft biopsy is only needed to decide on immunosuppression reduction in patients with an increased risk of acute rejection (i.e. the presence of DSA or known PRA positivity) or impaired baseline renal function of unknown origin. For all other patients, a preemptive treatment algorithm is recommended. To better reflect the continuum of BKPyV replication, immunosuppression reduction is recommended for KT patients with plasma BKPyV-DNAemia of 1000 c/ml sustained for more than three weeks (probable PyVAN), or more than 10,000 c/ml (presumptive PyVAN).

Finally, while supporting the potential of neutralizing antibodies as markers of increased risk, our case studies raise questions about the potential of neutralizing antibodies for prophylaxis or therapy. As commercial human i.v. immunoglobulin (Ig) has been shown to contain BKPyV neutralizing antibodies [27], recently monthly i.v. Ig injections during the first three critical months post-transplant was suggested as an initiative to prevent PyVAN development [33]. Others have suggested pre-vaccination of KT recipients with a multivalent VLP-based vaccine against all BKPyV sero−/genotypes [25]. However, the question has been raised whether or not the apparently beneficial neutralizing antibody activity observed in patients represents surrogates of their corresponding CD4 and/or CD8 activity (reviewed in [17, 20]). It is conceivable that the efficacy of administrating intravenous immunoglobulins may differ when given prophylactically before significant BKPyV spread in the renal allograft has occurred, or when administered in patients with significant BKPyV-DNAemia and PyVAN. Randomized controlled clinical trials are needed to address both situations. However, our study and that of others suggests that the antibody status pre-transplantation should be assessed in order to obtain meaningful results.

In this paired kidney case report, donor-derived transmission with rapid progression to presumptive and proven PyVAN probably occurred due to the combination of a recent BKPyV exposure in the donor and initial low levels of BKPyV-genotype I neutralizing antibodies in both recipients. More evidence is needed to evaluate whether measurement of neutralizing antibodies pre-transplant can be useful in organ allocation or more intense post-transplant screening. Until then, monthly screening for BKPyV-DNAemia followed by a rapid reduction of immunosuppression remains the standard measure to prevent allograft damage and loss due to PyVAN.