Si-Ying Zhou1, Wei Chen2, Su-Jin Yang3, Jian Li4, Jun-Ying Zhang3, He-da Zhang5, Shan-Liang Zhong6, Jin-Hai Tang1,7. 1. The First Clinical Medical College, Nanjing University of Chinese Medicine, Xianlin Road 138, Nanjing 210023, PR China. 2. Department of Head & Neck Surgery, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting 42, Nanjing 210029, PR China. 3. The First Clinical School of Nanjing Medical University, Nanjing 210029, PR China. 4. Department of General Surgery, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting 42, Nanjing 210029, PR China. 5. Department of General Surgery, School of Medicine, Southeast University, 87 Ding Jia Qiao, Nanjing, 210009, PR China. 6. Center of Clinical Laboratory Science, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting 42, Nanjing 210029, PR China. 7. Department of General Surgery, The First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, PR China.
Abstract
Aim: We aimed to explore the roles of circular RNA, circVAPA in regulating cell migration and invasion of breast cancer. Materials & methods: CircVAPA expression was detected in breast cancer tissues and cells. The role of circVAPA was evaluated by MTT assay, wound-healing and transwell assay. The relationship between circVAPA and miR-130a-5p and the location of circVAPA were explored. Results: We discovered that circVAPA was dysregulated in breast cancer tissues and cells. Ectopic circVAPA regulated breast cancer migration, invasion and proliferation. CircVAPA was mainly expressed in the cytoplasm and could act as a miRNA sponge for miR-130a-5p, but did not regulate its parental gene. Conclusion: CircVAPA may promote migration and invasion capacity of breast cancer via harboring miR-130a-5p.
Aim: We aimed to explore the roles of circular RNA, circVAPA in regulating cell migration and invasion of breast cancer. Materials & methods: CircVAPA expression was detected in breast cancer tissues and cells. The role of circVAPA was evaluated by MTT assay, wound-healing and transwell assay. The relationship between circVAPA and miR-130a-5p and the location of circVAPA were explored. Results: We discovered that circVAPA was dysregulated in breast cancer tissues and cells. Ectopic circVAPA regulated breast cancer migration, invasion and proliferation. CircVAPA was mainly expressed in the cytoplasm and could act as a miRNA sponge for miR-130a-5p, but did not regulate its parental gene. Conclusion: CircVAPA may promote migration and invasion capacity of breast cancer via harboring miR-130a-5p.
Authors: Jun Fang; Wen Hao Ji; Fang Zheng Wang; Tie Ming Xie; Lei Wang; Zhen Fu Fu; Zhun Wang; Feng Qin Yan; Qi Liang Shen; Zhi Min Ye Journal: J Cancer Date: 2021-03-05 Impact factor: 4.207