Su-Jin Yang1,2, Dan-Dan Wang1, Si-Ying Zhou3, Qian Zhang1,2, Jin-Yan Wang1,2, Shan-Liang Zhong4,5, He-da Zhang6, Xing-Yun Wang7, Xing Xia3, Wei Chen4,8, Su-Yu Yang9, Jia-Hua Hu4,5, Jian-Hua Zhao4,5, Jin-Hai Tang1. 1. Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China. 2. The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China. 3. Nanjing University of Chinese Medicine, Nanjing 210028, PR China. 4. The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, PR China. 5. Center of Clinical Laboratory, Nanjing Medical University Affiliated Cancer Hospital, Cancer Institute of Jiangsu Province, Nanjing 210009, PR China. 6. Department of General Surgery, Southeast University Medical School, Nanjing 210000, PR China. 7. Nanjing Maternal & Child Health Hospital, China Obstetrics & Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing 210004, PR China. 8. Department of Head & Neck Surgery, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Nanjing 210009, PR China. 9. Xuanwu Lake Community Health Service Center, Nanjing 210008, PR China.
Abstract
Aim: Circular RNAs (circRNAs) still have many potential functions in the process of tumor development that are not completely understood. The study aims to explore novel circRNAs and their mechanisms of action in breast cancer (BCa). Materials & methods: A combination strategy of RNA-sequencing (RNA-seq) technique, quantitative real-time PCR and bioinformatic analysis was employed to identify the potential mechanisms involving differentially expressed circRNAs in the serum exosomes and tissues of BCa patients. Results: The expression levels of hsa-circRNA-0005795 and hsa-circRNA-0088088 were significantly different both in serum exosomes and tissues and might function as competing endogenous RNAs and play vital roles in BCa development. Conclusion: We constructed two circRNA-miRNA networks and provided new insight into the prognosis and therapy of BCa using circRNAs from serum exosomes.
Aim: Circular RNAs (circRNAs) still have many potential functions in the process of tumor development that are not completely understood. The study aims to explore novel circRNAs and their mechanisms of action in breast cancer (BCa). Materials & methods: A combination strategy of RNA-sequencing (RNA-seq) technique, quantitative real-time PCR and bioinformatic analysis was employed to identify the potential mechanisms involving differentially expressed circRNAs in the serum exosomes and tissues of BCapatients. Results: The expression levels of hsa-circRNA-0005795 and hsa-circRNA-0088088 were significantly different both in serum exosomes and tissues and might function as competing endogenous RNAs and play vital roles in BCa development. Conclusion: We constructed two circRNA-miRNA networks and provided new insight into the prognosis and therapy of BCa using circRNAs from serum exosomes.
Entities:
Keywords:
breast cancer; ceRNA; circRNAs; exosomes; miRNA