Qingbin Wu1, Xiaochen Yuan2, Bingwei Li1, Jinna Yang3, Ruiqin Han4, Honggang Zhang5, Ruijuan Xiu1. 1. Key Laboratory for Microcirculation, Ministry of Health, Institute of Microcirculation, Chinese Academy Medical Sciences & Pecking Union Medical College, 5, Dong Dan San Tiao, Beijing, 100005, China. 2. Key Laboratory for Microcirculation, Ministry of Health, Institute of Microcirculation, Chinese Academy Medical Sciences & Pecking Union Medical College, 5, Dong Dan San Tiao, Beijing, 100005, China. yuanxc@imc.pumc.edu.cn. 3. Nine-Story Ward of International Medical Department, Peking Union Medical College Hospital, Beijing, China. 4. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China. 5. Key Laboratory for Microcirculation, Ministry of Health, Institute of Microcirculation, Chinese Academy Medical Sciences & Pecking Union Medical College, 5, Dong Dan San Tiao, Beijing, 100005, China. zhanghg1966126@163.com.
Abstract
OBJECTIVE: This study is to explore the exact roles of extracellular vesicle (EVs) miRNAs from brain microvascular pericytes in the pathogenesis of hypertension. RESULTS: Forty-eight significantly differentially expressed miRNAs were identified, of which 17 were found to be upregulated and 31 were found to be downregulated in brain microvascular pericytes of spontaneous hypertensive rats, compared with that of normotension Wistar Kyoto rats. The GO enrichment analysis verified that the target genes were enriched in signaling pathways and molecular functions, such as metal ion binding, nucleotide binding and ATP binding. The KEGG analysis indicated that the target genes were enriched in Linoleic acid, alpha-linolenic acid and sphingolipid metabolism pathways. CONCLUSIONS: Several EV derived miRNAs, such as miR-21-5p, let-7c-5p and let-7a-5p, were found to be abnormally expressed in brain microvascular pericytes obtained from spontaneous hypertensive rats, compared with that of normotension Wistar Kyoto rats. The results of our research provide more insights into the functional link between brain microvascular pericytes and the pathogenesis of hypertension.
OBJECTIVE: This study is to explore the exact roles of extracellular vesicle (EVs) miRNAs from brain microvascular pericytes in the pathogenesis of hypertension. RESULTS: Forty-eight significantly differentially expressed miRNAs were identified, of which 17 were found to be upregulated and 31 were found to be downregulated in brain microvascular pericytes of spontaneous hypertensiverats, compared with that of normotension Wistar Kyoto rats. The GO enrichment analysis verified that the target genes were enriched in signaling pathways and molecular functions, such as metal ion binding, nucleotide binding and ATP binding. The KEGG analysis indicated that the target genes were enriched in Linoleic acid, alpha-linolenic acid and sphingolipid metabolism pathways. CONCLUSIONS: Several EV derived miRNAs, such as miR-21-5p, let-7c-5p and let-7a-5p, were found to be abnormally expressed in brain microvascular pericytes obtained from spontaneous hypertensiverats, compared with that of normotension Wistar Kyoto rats. The results of our research provide more insights into the functional link between brain microvascular pericytes and the pathogenesis of hypertension.
Authors: Mi Ran Choi; Jasmin Sanghyun Han; Yeung-Bae Jin; Sang-Rae Lee; In Young Choi; Heejin Lee; Hyun Cho; Dai-Jin Kim Journal: Biol Sex Differ Date: 2020-11-23 Impact factor: 5.027