Silvia Bartollino1, Marisa Palazzo2, Francesco Semeraro3, Barbara Parolini4, Ciro Caruso5, Francesco Merolla6, Germano Guerra6, Ciro Costagliola6. 1. Department of Medicine and Health Science "V. Tiberio", University of Molise, 86100, Campobasso, Italy. silvia.bartollino@unimol.it. 2. Department of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, Italy. 3. Department of Medical and Surgical Specialties, Radiological Specialties and Public Health, University of Brescia, Brescia, Italy. 4. Eyecare Clinic, Brescia, Italy. 5. Corneal Transplant Center, Pellegrini Hospital, Naples, Italy. 6. Department of Medicine and Health Science "V. Tiberio", University of Molise, 86100, Campobasso, Italy.
Abstract
PURPOSE: The aim of this study has been to evaluate the protective effect of a topical antioxidant formulation containing riboflavin, d-α-tocopheryl polyethylene glycol (TPGS vitamin E), proline, glycine, lysine, and leucine against UV-B-induced damage in in vivo rabbit retina. METHODS: Twenty male albino rabbits were used. Animals were divided into four groups of five animals each. Control group did not receive any UV irradiation. The first group (IG) was irradiated with a UV-A lamp for 30 min; the second (IG30) and the third (IG60) groups received UV irradiation for 30 and 60 min, respectively, and were topically treated with 1 drop (approximately 50 µl) of the antioxidant formulation, every 15 min, starting 1 h before irradiation, until the end of the UC exposure. RESULTS: The retina of IG group showed extensive destruction of the retinal pigment epithelium (RPE) and of the cones and rods layer. The retina of G30 group showed a lesser destruction of both RPE and cones and rods layer. In the G60 group, retina showed an irregular thickening of the RPE, with massive edema of the inner and outer layer immediately adjacent together with a significant reduction of the photoreceptor number. CONCLUSION: Our results demonstrate that a topical application of eye drops containing riboflavin, d-α-tocopheryl polyethylene glycol (TPGS vitamin E), proline, glycine, lysine, and leucine counteracts UV retinal injury in exposed retina rabbits.
PURPOSE: The aim of this study has been to evaluate the protective effect of a topical antioxidant formulation containing riboflavin, d-α-tocopheryl polyethylene glycol (TPGS vitamin E), proline, glycine, lysine, and leucine against UV-B-induced damage in in vivo rabbit retina. METHODS: Twenty male albino rabbits were used. Animals were divided into four groups of five animals each. Control group did not receive any UV irradiation. The first group (IG) was irradiated with a UV-A lamp for 30 min; the second (IG30) and the third (IG60) groups received UV irradiation for 30 and 60 min, respectively, and were topically treated with 1 drop (approximately 50 µl) of the antioxidant formulation, every 15 min, starting 1 h before irradiation, until the end of the UC exposure. RESULTS: The retina of IG group showed extensive destruction of the retinal pigment epithelium (RPE) and of the cones and rods layer. The retina of G30 group showed a lesser destruction of both RPE and cones and rods layer. In the G60 group, retina showed an irregular thickening of the RPE, with massive edema of the inner and outer layer immediately adjacent together with a significant reduction of the photoreceptor number. CONCLUSION: Our results demonstrate that a topical application of eye drops containing riboflavin, d-α-tocopheryl polyethylene glycol (TPGS vitamin E), proline, glycine, lysine, and leucine counteracts UV retinal injury in exposed retina rabbits.
Entities:
Keywords:
Retina protection; Riboflavin; Topic formulation; UV damage; Vitamin E
Authors: Deepika Malik; Tiffany Hsu; Payam Falatoonzadeh; Javier Cáceres-del-Carpio; Mohamed Tarek; Marilyn Chwa; Shari R Atilano; Claudio Ramirez; Anthony B Nesburn; David S Boyer; Baruch D Kuppermann; S Michal Jazwinski; Michael V Miceli; Douglas C Wallace; Nitin Udar; M Cristina Kenney Journal: PLoS One Date: 2014-06-11 Impact factor: 3.240