| Literature DB >> 3191536 |
F P Gasparro1, R M Knobler, R L Edelson.
Abstract
The antitumor antibiotic gilvocarcin (GV) when photoactivated with UV radiation induced single strand breaks in superhelical pBR322 DNA. The optimal wavelengths for nicking DNA correlated with the absorbance maximum of GV near the visible region (398 nm). The response of lymphocytes to stimulation by phytohemagglutinin was reduced to 10% of controls at 0.10 ng/ml GV in combination with 3 J/cm2 of ultraviolet A (UVA) radiation. The potency of gilvocarcin is attributed to two factors: its strong tendency to intercalate with DNA (K = 6.6 X 10(5) M-1) and its intense absorption of UVA radiation (E398 = 11971 M-1 cm-1).Entities:
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Year: 1988 PMID： 3191536 DOI： 10.1016/0009-2797(88)90062-2
Source DB: PubMed Journal: Chem Biol Interact ISSN： 0009-2797 Impact factor: 5.192