Literature DB >> 31911131

Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing.

Jinjing Li1, Shunyan Hong1, Wanjin Chen2, Erwei Zuo3, Hui Yang4.   

Abstract

CRISPR-mediated genome editing is a revolutionary technology for genome manipulation that uses the CRISPR-Cas systems and base editors. Currently, poor efficiency and off-target problems have impeded the application of CRISPR systems. The on-target efficiency has been improved in several advanced versions of CRISPR systems, whereas the off-target detection still remains a key challenge. Here, we outline the different versions of CRISPR systems and off-target detection strategies, discuss the merits and limitations of off-target detection methods, and provide potential implications for further gene editing research.
Copyright © 2019 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Ltd. All rights reserved.

Keywords:  Base editors; CRISPR-Cas9; Gene editing; Off-target

Year:  2019        PMID： 31911131     DOI： 10.1016/j.jgg.2019.11.002

Source DB:  PubMed          Journal:  J Genet Genomics        ISSN： 1673-8527            Impact factor:   4.275


  11 in total

1.  Targeting Viral cccDNA for Cure of Chronic Hepatitis B.

Authors:  Gaëtan Ligat; Kaku Goto; Eloi Verrier; Thomas F Baumert
Journal:  Curr Hepatol Rep       Date:  2020-07-10

2.  Genome-wide specificity of prime editors in plants.

Authors:  Shuai Jin; Qiupeng Lin; Yingfeng Luo; Zixu Zhu; Guanwen Liu; Yunjia Li; Kunling Chen; Jin-Long Qiu; Caixia Gao
Journal:  Nat Biotechnol       Date:  2021-04-15       Impact factor: 54.908

Review 3.  Genetic engineering of T cells for immunotherapy.

Authors:  Gavin I Ellis; Neil C Sheppard; James L Riley
Journal:  Nat Rev Genet       Date:  2021-02-18       Impact factor: 59.581

Review 4.  Latest Developed Strategies to Minimize the Off-Target Effects in CRISPR-Cas-Mediated Genome Editing.

Authors:  Muhammad Naeem; Saman Majeed; Mubasher Zahir Hoque; Irshad Ahmad
Journal:  Cells       Date:  2020-07-02       Impact factor: 6.600

Review 5.  Future Approaches for Treating Chronic Myeloid Leukemia: CRISPR Therapy.

Authors:  Elena Vuelta; Ignacio García-Tuñón; Patricia Hernández-Carabias; Lucía Méndez; Manuel Sánchez-Martín
Journal:  Biology (Basel)       Date:  2021-02-04

Review 6.  The Potential of CRISPR/Cas9 Gene Editing as a Treatment Strategy for Inherited Diseases.

Authors:  Sameh A Abdelnour; Long Xie; Abdallah A Hassanin; Erwei Zuo; Yangqing Lu
Journal:  Front Cell Dev Biol       Date:  2021-12-15

7.  BMAL1 moonlighting as a gatekeeper for LINE1 repression and cellular senescence in primates.

Authors:  Chuqian Liang; Qiong Ke; Zunpeng Liu; Jie Ren; Weiqi Zhang; Jianli Hu; Zehua Wang; Hong Chen; Kai Xia; Xingqiang Lai; Qiaoran Wang; Kuan Yang; Wei Li; Zeming Wu; Chao Wang; Haoteng Yan; Xiaoyu Jiang; Zhejun Ji; Miyang Ma; Xiao Long; Si Wang; Huating Wang; Hao Sun; Juan Carlos Izpisua Belmonte; Jing Qu; Andy Peng Xiang; Guang-Hui Liu
Journal:  Nucleic Acids Res       Date:  2022-04-08       Impact factor: 16.971

Review 8.  Chronic myeloid leukemia stem cells: targeting therapeutic implications.

Authors:  Hanieh Mojtahedi; Niloufar Yazdanpanah; Nima Rezaei
Journal:  Stem Cell Res Ther       Date:  2021-12-18       Impact factor: 6.832

Review 9.  CRISPR/Cas9 Gene-Editing in Cancer Immunotherapy: Promoting the Present Revolution in Cancer Therapy and Exploring More.

Authors:  Xuejin Ou; Qizhi Ma; Wei Yin; Xuelei Ma; Zhiyao He
Journal:  Front Cell Dev Biol       Date:  2021-05-20

Review 10.  Genome-Editing Strategies for Treating Human Retinal Degenerations.

Authors:  Joel Quinn; Ayesha Musa; Ariel Kantor; Michelle E McClements; Jasmina Cehajic-Kapetanovic; Robert E MacLaren; Kanmin Xue
Journal:  Hum Gene Ther       Date:  2020-11-19       Impact factor: 5.695
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