Guanmin Zheng1,2, Qingxia Lu2, Fangyu Wang2, Guangxu Xing2, Hua Feng2, Qianyue Jin2, Zhenhua Guo2, Man Teng2, Huifang Hao2, Dongliang Li1,2, Xin Wei1,2, Yuhang Zhang1,2, Ruiguang Deng2, Gaiping Zhang3,4,5. 1. College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China. 2. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Huayuan Road No. 116, Zhengzhou, 450002, China. 3. College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China. zhanggaip@126.com. 4. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Huayuan Road No. 116, Zhengzhou, 450002, China. zhanggaip@126.com. 5. Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, Jiangsu, China. zhanggaip@126.com.
Abstract
BACKGROUND: Porcine circovirus type 2 (PCV2) is the pathogen of porcine circovirus associated diseases (PCVAD) and one of the main pathogens in the global pig industry, which has brought huge economic losses to the pig industry. In recent years, there has been limited research on the prevalence of PCV2 in Henan Province. This study investigated the genotype and evolution of PCV2 in this area. RESULTS: We collected 117 clinical samples from different regions of Henan Province from 2015 to 2018. Here, we found that the PCV2 infection rate of PCV2 was 62.4%. Thirty-seven positive clinical samples were selected to amplify the complete genome of PCV2 and were sequenced. Based on the phylogenetic analysis of PCV2 ORF2 and complete genome, it was found that the 37 newly detected strains belonged to PCV2a (3 of 37), PCV2b (21 of 37) and PCV2d (13 of 37), indicating the predominant prevalence of PCV2b and PCV2d strains. In addition, we compared the amino acid sequences and found several amino acid mutation sites among different genotypes. Furthermore, the results of selective pressure analysis showed that there were 5 positive selection sites. CONCLUSIONS: This study indicated the genetic diversity, molecular epidemiology and evolution of PCV2 genotypes in Henan Province during 2015-2018.
BACKGROUND:Porcine circovirus type 2 (PCV2) is the pathogen of porcine circovirus associated diseases (PCVAD) and one of the main pathogens in the global pig industry, which has brought huge economic losses to the pig industry. In recent years, there has been limited research on the prevalence of PCV2 in Henan Province. This study investigated the genotype and evolution of PCV2 in this area. RESULTS: We collected 117 clinical samples from different regions of Henan Province from 2015 to 2018. Here, we found that the PCV2infection rate of PCV2 was 62.4%. Thirty-seven positive clinical samples were selected to amplify the complete genome of PCV2 and were sequenced. Based on the phylogenetic analysis of PCV2ORF2 and complete genome, it was found that the 37 newly detected strains belonged to PCV2a (3 of 37), PCV2b (21 of 37) and PCV2d (13 of 37), indicating the predominant prevalence of PCV2b and PCV2d strains. In addition, we compared the amino acid sequences and found several amino acid mutation sites among different genotypes. Furthermore, the results of selective pressure analysis showed that there were 5 positive selection sites. CONCLUSIONS: This study indicated the genetic diversity, molecular epidemiology and evolution of PCV2 genotypes in Henan Province during 2015-2018.
Authors: J Ellis; L Hassard; E Clark; J Harding; G Allan; P Willson; J Strokappe; K Martin; F McNeilly; B Meehan; D Todd; D Haines Journal: Can Vet J Date: 1998-01 Impact factor: 1.008
Authors: Phat Xuan Dinh; Minh Nam Nguyen; Hien The Nguyen; Vu Hoang Tran; Quy Dinh Tran; Kim Hoang Dang; Dai Tan Vo; Hien Thanh Le; Nam Thi Thu Nguyen; Toan Tat Nguyen; Duy Tien Do Journal: Arch Virol Date: 2021-01-03 Impact factor: 2.574