| Literature DB >> 31907757 |
Yang Yu1,2, Ying Chen3, Yi-Jing Zheng2, Qi-Hao Weng2, Si-Pin Zhu2, Dong-Sheng Zhou4.
Abstract
LncRNA TUG1 has the potential to promote the osteogenic differentiation of several cells, but the role of lncRNA TUG1 in osteogenic differentiation of tendon stem/progenitor cells (TSPCs) is still unknown. This study aims to determine the role of lncRNA TUG1 in osteogenic differentiation of TSPCs. bFGF, RUNX2, and Osterix protein expressions were detected by western blot. LncRNA TUG1 and bFGF expression was detected by qRT-PCR. RNA immunoprecipitation (RIP) assay was used to confirm the interaction between TUG1 and bFGF2. Ubiquitination assay was used to determine the ubiquitination of bFGF protein. During osteogenic differentiation, the protein expression of bFGF was significantly downregulated in TSPCs, and the expression of TUG1 was significantly elevated in TSPCs. Interfering TUG1 or overexpressing bFGF suppressed osteogenic differentiation of TSPCs. In addition, lncRNA TUG1 interacted with bFGF, and lncRNA TUG1 promoted the ubiquitination of bFGF protein. We also determined that lncRNA TUG1 downregulated bFGF protein expression through promoting the ubiquitination of bFGF. LncRNA TUG1 promoted the osteogenic differentiation of TSPCs through promoting bFGF ubiquitination.Entities:
Keywords: LncRNA TUG1; Osteogenic differentiation; Tendon stem/progenitor cells; bFGF
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Year: 2020 PMID： 31907757 DOI： 10.1007/s11626-019-00410-y
Source DB: PubMed Journal: In Vitro Cell Dev Biol Anim ISSN： 1071-2690 Impact factor: 2.416