Literature DB >> 31907545

Clinical Utility of Universal Broad-Range Polymerase Chain Reaction Amplicon Sequencing for Pathogen Identification: A Retrospective Cohort Study.

Andrew D Kerkhoff1, Rachel L Rutishauser2, Steve Miller3, Jennifer M Babik4.   

Abstract

We assessed the real-world utility of universal broad-range polymerase chain reaction sequencing for pathogen detection. Among 1062 clinical samples, 107/1062 (10.1%) had a clinically significant, positive result, with substantial variation by specimen type. Clinical management was changed in 44/1062 (4.1%). These data can help maximize utility of this emerging diagnostic.
© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

Entities:  

Keywords:  DNA sequencing; broad-range universal PCR; molecular diagnostics; pathogen identification

Mesh：

Year:  2020        PMID： 31907545      PMCID： PMC7486847          DOI： 10.1093/cid/ciz1245

Source DB:  PubMed          Journal:  Clin Infect Dis        ISSN： 1058-4838            Impact factor:   9.079


  11 in total

1.  16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates.

Authors:  M Drancourt; C Bollet; A Carlioz; R Martelin; J P Gayral; D Raoult
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

2.  Rapid and accurate identification of mycobacteria by sequencing hypervariable regions of the 16S ribosomal RNA gene.

Authors:  Xiang Y Han; Audrey S Pham; Jeffrey J Tarrand; Pramila K Sood; Rajyalakshmi Luthra
Journal:  Am J Clin Pathol       Date:  2002-11       Impact factor: 2.493

3.  Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting.

Authors:  Tim Schuurman; Richard F de Boer; Anna M D Kooistra-Smid; Anton A van Zwet
Journal:  J Clin Microbiol       Date:  2004-02       Impact factor: 5.948

4.  Clinical Metagenomic Sequencing for Diagnosis of Meningitis and Encephalitis.

Authors:  Michael R Wilson; Hannah A Sample; Kelsey C Zorn; Shaun Arevalo; Guixia Yu; John Neuhaus; Scot Federman; Doug Stryke; Benjamin Briggs; Charles Langelier; Amy Berger; Vanja Douglas; S Andrew Josephson; Felicia C Chow; Brent D Fulton; Joseph L DeRisi; Jeffrey M Gelfand; Samia N Naccache; Jeffrey Bender; Jennifer Dien Bard; Jamie Murkey; Magrit Carlson; Paul M Vespa; Tara Vijayan; Paul R Allyn; Shelley Campeau; Romney M Humphries; Jeffrey D Klausner; Czarina D Ganzon; Fatemeh Memar; Nicolle A Ocampo; Lara L Zimmermann; Stuart H Cohen; Christopher R Polage; Roberta L DeBiasi; Barbara Haller; Ronald Dallas; Gabriela Maron; Randall Hayden; Kevin Messacar; Samuel R Dominguez; Steve Miller; Charles Y Chiu
Journal:  N Engl J Med       Date:  2019-06-13       Impact factor: 91.245

5.  Broad-range 16S rRNA gene polymerase chain reaction for diagnosis of culture-negative bacterial infections.

Authors:  Silvana K Rampini; Guido V Bloemberg; Peter M Keller; Andrea C Büchler; Günter Dollenmaier; Roberto F Speck; Erik C Böttger
Journal:  Clin Infect Dis       Date:  2011-10-05       Impact factor: 9.079

Review 6.  Clinical Metagenomic Next-Generation Sequencing for Pathogen Detection.

Authors:  Wei Gu; Steve Miller; Charles Y Chiu
Journal:  Annu Rev Pathol       Date:  2018-10-24       Impact factor: 23.472

7.  Evaluation of 16S rRNA gene PCR sensitivity and specificity for diagnosis of prosthetic joint infection: a prospective multicenter cross-sectional study.

Authors:  Pascale Bémer; Chloé Plouzeau; Didier Tande; Julie Léger; Bruno Giraudeau; Anne Sophie Valentin; Anne Jolivet-Gougeon; Pascal Vincent; Stéphane Corvec; Sophie Gibaud; Marie Emmanuelle Juvin; Genevieve Héry-Arnaud; Carole Lemarié; Marie Kempf; Laurent Bret; Roland Quentin; Carine Coffre; Gonzague de Pinieux; Louis Bernard; Christophe Burucoa
Journal:  J Clin Microbiol       Date:  2014-07-23       Impact factor: 5.948

8.  Molecular diagnosis of infective endocarditis by real-time broad-range polymerase chain reaction (PCR) and sequencing directly from heart valve tissue.

Authors:  Mercedes Marín; Patricia Muñoz; Mónica Sánchez; Marina Del Rosal; Luis Alcalá; Marta Rodríguez-Créixems; Emilio Bouza
Journal:  Medicine (Baltimore)       Date:  2007-07       Impact factor: 1.889

Review 9.  Microbial Identification Using DNA Target Amplification and Sequencing: Clinical Utility and Impact on Patient Management.

Authors:  Tinzar Basein; Bradley J Gardiner; Gabriela M Andujar Vazquez; Andrew S Joel Chandranesan; Arthur R Rabson; Shira Doron; David R Snydman
Journal:  Open Forum Infect Dis       Date:  2018-10-10       Impact factor: 3.835

10.  Similar efficacy of broad-range ITS PCR and conventional fungal culture for diagnosing fungal infections in non-immunocompromised patients.

Authors:  Silvana K Rampini; Andrea Zbinden; Roberto F Speck; Guido V Bloemberg
Journal:  BMC Microbiol       Date:  2016-06-28       Impact factor: 3.605
View more
  6 in total

1.  High Clinical Impact of Broad-Range Fungal PCR in Suspected Fungal Sinusitis.

Authors:  Andrew Bryan; James A Mays; Joshua A Lieberman; Karen Stephens; Kyoko Kurosawa; Patrick C Mathias; Dhruba SenGupta; Lori Bourassa; Stephen J Salipante; Brad T Cookson
Journal:  J Clin Microbiol       Date:  2021-08-18       Impact factor: 5.948

2.  Diagnostic Value of 16S Ribosomal RNA Gene Polymerase Chain Reaction/Sanger Sequencing in Clinical Practice.

Authors:  Madiha Fida; Sarwat Khalil; Omar Abu Saleh; Douglas W Challener; Muhammad Rizwan Sohail; Joshua N Yang; Bobbi S Pritt; Audrey N Schuetz; Robin Patel
Journal:  Clin Infect Dis       Date:  2021-09-15       Impact factor: 20.999

3.  Utility of Broad-Range PCR Sequencing for Infectious Diseases Clinical Decision Making: a Pediatric Center Experience.

Authors:  Caitlin Naureckas Li; Mari M Nakamura
Journal:  J Clin Microbiol       Date:  2022-04-11       Impact factor: 11.677

4.  Determining the Clinical Utility of 16S rRNA Sequencing in the Management of Culture-Negative Pediatric Infections.

Authors:  Peter Paul C Lim; Lisa M Stempak; Sindhoosha Malay; LeAnne N Moore; Sree Sarah S Cherian; Ankita P Desai
Journal:  Antibiotics (Basel)       Date:  2022-01-26

5.  Mycobacterium avium Complex Infection as a Rare Cause of Cerebral Mass Lesion and IRIS in a Patient With AIDS: Case Report and Review of the Literature.

Authors:  Courtney Lane-Donovan; Emma Bainbridge; John Szumowski; Andrew D Kerkhoff; Michael J Peluso
Journal:  Open Forum Infect Dis       Date:  2021-10-30       Impact factor: 3.835

6.  Fungal osteomyelitis and septic arthritis in an immune competent man: The first report of invasive osteoarticular infection due to Scedosporium dehoogii.

Authors:  Daniel S Krauth; Brian T Barlow; Catherine M Berjohn
Journal:  Med Mycol Case Rep       Date:  2021-06-19
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.