Kam Chau Yung1, Cheng Wei Xu2, Ze Wen Zhang1, Wen Jun Yu1, Qian Li1, Xian Ru Xu1, Ya Fei Han3, Xin Jia Wang3, Jun Yin1,4,5. 1. Division of Hematology, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China. 2. Hemodialysis Room, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China. 3. Department of Spine Surgery, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China. 4. Department of Clinical Laboratory Medicine, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China. 5. Translational Medicine Research Center, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
Abstract
Objective: The expression of glucocorticoid receptors within platelets from newly diagnosed Immune Thrombocytopenia (ITP) patients in the adult was investigated. Methods: GR expression in platelets from newly diagnosed ITP patients and healthy controls was measured using flow cytometry. Subsequently, platelets RNA and proteins were isolated and used for confirming the flow cytometry results by using RT-qPCR and ELISA. Results: Flow cytometry showed that the percentages of platelets expressing GRα and GRβ from ITP patients were significantly higher than those from healthy controls (P < 0.05). qPCR and ELISA confirmed that GRα and GRβ were increased at both RNA transcription and protein expression levels within platelets from ITP patients compared with healthy controls. Conclusion: We speculated that the up-regulation of glucocorticoid receptor within platelets may be an important biological feature of platelets in patients with ITP, and may also play an important role in the treatment of ITP, which is worthy of further study.
Objective: The expression of glucocorticoid receptors within platelets from newly diagnosed Immune Thrombocytopenia (ITP) patients in the adult was investigated. Methods: GR expression in platelets from newly diagnosed ITP patients and healthy controls was measured using flow cytometry. Subsequently, platelets RNA and proteins were isolated and used for confirming the flow cytometry results by using RT-qPCR and ELISA. Results: Flow cytometry showed that the percentages of platelets expressing GRα and GRβ from ITP patients were significantly higher than those from healthy controls (P < 0.05). qPCR and ELISA confirmed that GRα and GRβ were increased at both RNA transcription and protein expression levels within platelets from ITP patients compared with healthy controls. Conclusion: We speculated that the up-regulation of glucocorticoid receptor within platelets may be an important biological feature of platelets in patients with ITP, and may also play an important role in the treatment of ITP, which is worthy of further study.