Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Chondrocytes-derived exosomal miR-8485 regulated the Wnt/β-catenin pathways to promote chondrogenic differentiation of BMSCs.

Literature DB >> 31898972

Chondrocytes-derived exosomal miR-8485 regulated the Wnt/β-catenin pathways to promote chondrogenic differentiation of BMSCs.

Zeng Li¹, Yingjie Wang¹, Shuai Xiang², Zhibo Zheng¹, Yanyan Bian¹, Bin Feng¹, Xisheng Weng³.

Abstract

In indirect co-culture system, chondrocytes can induce differentiation of bone marrow mesenchymal stem cells (BMSCs) to chondrocytes without additional inducer. The participation of microRNAs (miRNAs) may take part in the chondrogenic differentiation. Present study aimed to investigate the effect and mechanism of chondrocytes-derived exosomal miRNA in BMSCs chondrogenic differentiation. Our data showed that miR-8485 was the exosomal miRNA derived from chondrocytes and transmitted to BMSCs. Functionally, miR-8485 silence in chondrocytes impaired exosome-induced chondrogenic differentiation of BMSCs. Mechanistically, exosomal miR-8485 targeted GSK3B to repress GSK-3β expression and targeted DACT1 to induce p-GSK-3β (Ser9), activating Wnt/β-catenin pathways. Our study firstly showed that chondrocytes-derived exosomal miR-8485 regulated the Wnt/β-catenin pathways to promote chondrogenic differentiation of BMSCs, providing innovative thoughts for cartilage reconstruction.

Entities: Chemical Gene

Keywords: BMSC; Chondrogenic differentiation; GSK-3β; Wnt/β-catenin pathways; miR-8485

Mesh：

Substances：

Year: 2019 PMID： 31898972 DOI： 10.1016/j.bbrc.2019.12.065

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

Keyword Cloud
Cited

22 in total

Review 1. Activation of SIRT-1 Signalling in the Prevention of Bipolar Disorder and Related Neurocomplications: Target Activators and Influences on Neurological Dysfunctions.

Authors: Bidisha Rajkhowa; Sidharth Mehan; Pranshul Sethi; Aradhana Prajapati
Journal: Neurotox Res Date: 2022-02-14 Impact factor: 3.911

10. [Effect of hypoxia inducible factor 1α overexpression on differentiation of stem cells derived from human exfoliated deciduous teeth into vascular endothelial cells].

Authors: Yuanyuan Li; Dan Chen; Bei Wu
Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi Date: 2021-06-15

Chondrocytes-derived exosomal miR-8485 regulated the Wnt/β-catenin pathways to promote chondrogenic differentiation of BMSCs.

Review 1. Activation of SIRT-1 Signalling in the Prevention of Bipolar Disorder and Related Neurocomplications: Target Activators and Influences on Neurological Dysfunctions.

2. Exosomes: roles and therapeutic potential in osteoarthritis.

3. Exosomes: roles and therapeutic potential in osteoarthritis.

4. Fibroblast Growth Factor 9 (FGF9) negatively regulates the early stage of chondrogenic differentiation.

Review 5. Mesenchymal Stromal Cell-Derived Extracellular Vesicles - Silver Linings for Cartilage Regeneration?

Review 6. Extracellular vesicles: a promising cell-free therapy for cartilage repair.

Review 7. Role of Exosomal Non-Coding RNAs in Bone-Related Diseases.

Review 8. Harnessing Tissue-derived Extracellular Vesicles for Osteoarthritis Theranostics.

9. Scrapie-Responsive Gene 1 Promotes Chondrogenic Differentiation of Umbilical Cord Mesenchymal Stem Cells via Wnt5a.

10. [Effect of hypoxia inducible factor 1α overexpression on differentiation of stem cells derived from human exfoliated deciduous teeth into vascular endothelial cells].