Literature DB >> 3189809

Determination of nitrilotriacetate in biological matrices using ion exclusion chromatography.

R P Schneider1, F Zürcher, T Egli, G Hamer.   

Abstract

A sensitive method for the determination of nitrilotriacetate in biological growth media and cell-free extracts by ion exclusion chromatography is described using HCl as an eluant. The eluant conductivity was chemically suppressed with a membrane suppressor and a conductivity detector was used for subsequent detection. The membrane was continuously regenerated with a tetrabutylammoniumhydroxide solution. The detection limit for nitrilotriacetate in cell-free extract was 11 mg/liter, while for nitrilotriacetate in growth media it was 1 mg/liter. Interference by compounds present in biological matrices with the determination is discussed.

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Year:  1988        PMID： 3189809     DOI： 10.1016/0003-2697(88)90190-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  5 in total

1.  Cloning and characterization of the genes encoding nitrilotriacetate monooxygenase of Chelatobacter heintzii ATCC 29600.

Authors:  H R Knobel; T Egli; J R van der Meer
Journal:  J Bacteriol       Date:  1996-11       Impact factor: 3.490

2.  Dynamics of Substrate Consumption and Enzyme Synthesis in Chelatobacter heintzii during Growth in Carbon-Limited Continuous Culture with Different Mixtures of Glucose and Nitrilotriacetate.

Authors:  M Bally; T Egli
Journal:  Appl Environ Microbiol       Date:  1996-01       Impact factor: 4.792

3.  Anaerobic degradation of nitrilotriacetate (NTA) in a denitrifying bacterium: purification and characterization of the NTA dehydrogenase-nitrate reductase enzyme complex.

Authors:  U Jenal-Wanner; T Egli
Journal:  Appl Environ Microbiol       Date:  1993-10       Impact factor: 4.792

4.  Purification and characterization of a two-component monooxygenase that hydroxylates nitrilotriacetate from "Chelatobacter" strain ATCC 29600.

Authors:  T Uetz; R Schneider; M Snozzi; T Egli
Journal:  J Bacteriol       Date:  1992-02       Impact factor: 3.490

5.  Isolation and growth of a bacterium able to degrade nitrilotriacetic acid under denitrifying conditions.

Authors:  U Wanner; J Kemmler; H U Weilenmann; T Egli; T el-Banna; G Auling
Journal:  Biodegradation       Date:  1990       Impact factor: 3.909
  5 in total

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