Imatinib inhibits oxidative stress response in spinal cord injury rats by activating Nrf2/HO-1 signaling pathway.

Effect of imatinib on rats with spinal cord injury (SCI) was investigated through the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. Forty-eight Sprague-Dawley rats were randomly divided into sham operation group (n=12), model group (n=12), imatinib group (n=12) and inhibitor group (n=12). The results of immunohistochemistry showed that in comparison with sham operation group, the other three groups had overtly increased positive expression level of Bax and evidently reduced positive expression level of Bcl-2 (P<0.05). Compared with those in model group and inhibitor group, the positive expression level of Bax was distinctly lower, while that of Bcl-2 was notably increased in imatinib group (P<0.05). According to western blot analysis, the protein expression levels of Nrf2 and HO-1 were obviously higher in the other three groups than those in sham operation group (P<0.05), and they were remarkably higher in imatinib group than those in model group and inhibitor group (P<0.05). The results of qPCR assay revealed that the Nrf2 and HO-1 mRNA expression levels were markedly elevated in the other three groups compared with those in sham operation group (P<0.05). Based on ELISA, the other three groups exhibited notably raised content of IL-6, TNF-α, ROS and SOD compared with sham operation group (P<0.05), and imatinib group displayed remarkably decreased content of IL-6, TNF-α and ROS and markedly elevated SOD content in comparison with model group and inhibitor group (P<0.05). The results of TUNEL assay demonstrated that the rate of apoptosis was significantly raised in the other three groups compared with that in sham operation group (P<0.05), and it declined obviously in imatinib group compared with that in model group and inhibitor group (P<0.05). Imatinib inhibits oxidative stress response in SCI rats by activating the Nrf2/HO-1 signaling pathway, thus repressing apoptosis and inflammation. Copyright: © Liu et al.

Introduction

Spinal cord injury (SCI) refers to spinal cord and cauda equina injuries induced by various factors, resulting in motor dysfunction, sensory dysfunction, nerve reflex dysfunction and sphincter dysfunction in the limb below the level of injury. It is a challenge in clinical treatment and one of the research hotspots all over the world. With the development of urbanization and transportation, the incidence rate of injuries caused by high-altitude falling and traffic accidents is on the rise, and thus the morbidity rate of SCI is increasingly high. Epidemiological studies have manifested that the incidence rate of SCI is (27–83)/1 million in the United States and (10–30)/1 million in Europe. Given this, it is urgent to find effective ways of treating SCI and study the pathological mechanism of SCI.

The pathological responses of SCI are complicated, including inflammation, peroxidation, stress response, apoptosis and necrosis (1,2). Oxidative stress response plays a vital role in SCI. Studies have discovered that (3,4) in the early stage of SCI, reactive oxygen species (ROS) is released in quantity due to the denaturation and decomposition of fatty acids in a damaged environment, thus mediating oxidative stress response. Moreover, oxidative stress response further promotes the release of inflammatory factors such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), aggravating neuronal apoptosis. The nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway, an important anti-oxidative stress signaling pathway, is activated by injuries to modulate the release of antioxidant substances [superoxide dismutase (SOD) and HO-1], thereby inhibiting oxidative stress (5,6).

Imatinib is a tyrosine kinase inhibitor, which is commonly applied in the treatment of chronic lymphocytic leukemia. A study found that imatinib is capable of protecting the blood-brain barrier and relieving inflammation after central nervous system injury (7). However, the role of imatinib in SCI and relevant mechanism of action still remain unclear. This study, therefore, explored the effect of imatinib on SCI through the Nrf2/HO-1 signaling pathway.

Materials and methods

Laboratory animals and grouping

Forty-eight Sprague-Dawley rats (half male and half female) weighing 220±20 g were purchased form Shanghai SLAC Laboratory Animal Co., Ltd., with the license no. of SCXK (Shanghai) 2014-0003. The above 48 rats were divided into sham operation group (n=12), model group (n=12), imatinib group (n=12) and inhibitor group (n=12) using a random number table. This study was approved by the Animal Ethics Committee of Qinghai Provincial People's Hospital Animal Center (Xining, China).

Laboratory reagents and instruments

Nrf2 inhibitor ML385 (Sigma-Aldrich; Merck KGaA), primary antibodies [anti-B-cell lymphoma-2 (Bcl-2) antibody, anti-Bcl-2-associated X protein (Bax) antibody, anti-Nrf2 antibody and anti-HO-1 antibody (Abcam)], enzyme-linked immunosorbent assay (ELISA) kit (Wuhan Boster Biological Technology Co., Ltd.), AceQ quantitative polymerase chain reaction (qPCR) SYBR-Green Master Mix kit and HiScript II Q RT SuperMix for qPCR (+gDNA wiper) kit (Vazyme), terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) apoptosis kit (Sigma-Aldrich; Merck KGaA), optical microscope (Leica DMI 4000B/DFC425C; Leica Microsystems GmbH), fluorescence qPCR instrument (ABI 7500; Applied Biosystems; Thermo Fisher Scientific, Inc.), and ImageLab and Image-Pro image analysis systems (Bio-Rad Laboratories).

Modeling

The rats were anesthetized with 7% chloral hydrate at a dose of 3 ml/kg via intraperitoneal injection, spinous processes at the T9-T11 level were located, and local skin was disinfected. Then, the skin and muscles were cut open successively to peel off the muscles and ligaments attaching to the spinous processes and transverse processes, and a rongeur was used to remove the vertebral plate. Next, laminectomy was performed, and the spinal cord at the T9-T11 level was carefully exposed. Thereafter, the rats were fixed on a SCI impactor, with the impacting rod aiming at the T10 spinal cord, and then the impacting rod fell from a height of 12.5 mm to impact the T10 spinal cord via freely falling body motion. Convulsive tremor of the four limbs and swinging of the tail of rats observed suggested successful modeling. After that, the wound was washed, sutured and bandaged, and the rats were housed in single cages.

Treatment in each group

In sham operation group, laminectomy was conducted, the spinal cord was not damaged, and then normal saline was intraperitoneally injected after operation. In model group, the model of SCI was prepared, and the same volume of normal saline was intraperitoneally injected daily after modeling. In imatinib group, imatinib was intraperitoneally injected daily with an injection volume of 50 mg/kg after the rats were prepared into models of SCI. The rats in inhibitor group were prepared into SCI models and then intraperitoneally injected with the inhibitor ML385 every day with an injection volume of 100 mg/kg and then (1 h later) with imatinib at a dose of 50 mg/kg. The materials were taken after consecutive 7 days of intervention.

Collection of materials

Collection of materials was performed at 7 days after intervention. After successful anesthesia, 6 rats in each group were perfused and fixed with paraformaldehyde. Next, the spinal cord tissues were collected, fixed in 4% paraformaldehyde at 4°C for 48 h, and prepared into paraffin tissue sections for immunohistochemistry and TUNEL. As to the remaining 6 rats in each group, the spinal cord tissues were taken directly and placed in Eppendorf (EP) tubes for western blotting and qPCR.

Immunohistochemistry

The paraffin-embedded tissues were made into sections (5 µm in thickness), placed in 42°C warm water for spreading, collected using slides, baked, and prepared into paraffin tissue sections. Next, the paraffin tissue sections were sequentially soaked in xylene solution and graded ethanols, conventionally deparaffinized and hydrated. Thereafter, they were immersed in citric acid buffer and heated in a microwave 3 times (3 min of heating + 5 min of simmer each time) for complete antigen retrieval. After rinsing, the specimens were dropwise added with endogenous peroxidase blocker for reaction for 10 min, followed by rinsing. Then, goat serum was added dropwise for 20 min of blocking. Subsequently, the goat serum blocking solution was removed, and the specimens were added with anti-Bcl-2 primary antibody (1:200) and anti-Bax primary antibody (1:200) and placed in a refrigerator at 4°C overnight. The next day, the specimens were rinsed, dropwise added with secondary antibody solution for 10 min of incubation, thoroughly rinsed and reacted with streptomycin-biotin-peroxidase solution for 10 min, followed by color development with diaminobenzidine (DAB) added in drops. Lastly, the nuclei were counterstained with hematoxylin, mounted and observed.

Western blotting

The cryopreserved spinal cord tissues were added with lysis buffer, followed by ice-bath for 1 h and then centrifugation at 14,000 × g at 4°C for 10 min using a centrifuge. Next, the proteins were quantified via bicinchoninic acid (BCA) method (Abcam), and the protein concentration was calculated based on the absorbance values and standard curves obtained by a microplate reader. Thereafter, the proteins were denatured and separated through dodecyl sulfate, sodium salt-polyacrylamide gel electrophoresis (SDS-PAGE) during which the position of the Marker protein was observed. When the Marker protein was in a straight line at the bottom of the glass plate, the separation was stopped. Then, the protein was transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore), and added with blocking solution for 1.5 h of reaction, and incubated with anti-Nrf2 primary antibody (1:1,000), anti-HO-1 antibody (1:1,000) and secondary antibody (1:1,000) sequentially. After that, the membrane was rinsed and added with chemiluminescent reagent for development for 1 min in the dark.

qPCR assay

Total ribonucleic acid (RNA) was extracted and then reverse transcribed into complementary deoxyribonucleic acids (cDNAs) using the reverse transcription kit. The reaction system was 20 µl. Reaction conditions: Reaction at 51°C for 2 min, pre-denaturation at 96°C for 10 min, denaturation at 96°C for 10 sec, annealing at 60°C for 30 sec, 40 cycles. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal reference, and the relative expression level of related messenger RNA (mRNA) was calculated. The primer sequences are shown in Table I.

Table I.

Primer sequences.

Name	Primer sequence
Nrf2	F: 5′-TCCACCAAGAAGCTGAGCGAG-3′
	R: 5′-GTCCAGCCCATGATGGTTCT-3′
HO-1	F: 5′-CCTCGTGCTGTCGGACCCATA-3′
	R: 5′-CAGGCTTGTGCTCTGCTTGTGA-3′
GADPH	F: 5′-ACGGCAAGTTCAACGGCACAG-3′
	R: 5′-GAAGACGCCAGTAGACTCCACGAC-3′

F, forward; R, reverse; Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

TUNEL apoptosis assay

Appotosis of spinal cord tissues were detected in accordance with the instructions of the TUNEL appotosis kit.

ELISA

The spinal cord tissues collected were ground. Then, ELISA was performed as per the instructions of the ELISA kit: the samples were loaded and added with standard substance, biotinylated antibody working solution and enzyme conjugate working solution, followed by washing of the plate. Lastly, the microplate reader was utilized for detection at 450 nm.

Statistical analysis

In this study, Statistical Product and Service Solutions (SPSS) 20.0 (IBM Corp.) software was used for statistical analysis. Measurement data were expressed as mean ± standard deviation. t-test was utilized for data with normal distribution and homogeneity of variance, corrected-t-test for those with normal distribution and heterogeneity of variance, and non-parametric test for those without normal distribution and homogeneity of variance. Rank sum test was applied for ranked data. For enumeration data, Chi-square test was employed.

Results

Results of immunohistochemistry

The positive expression color was tan (Fig. 1). In sham operation group, the positive expression of Bax was few, while that of Bcl-2 was more. These two indicators showed opposite tendencies in model group. The statistical results shown in Fig. 2 revealed that compared with those in sham operation group, the average optical density of Bax positive expression was significantly increased in the other three groups, while that of Bcl-2 positive expression was overtly decreased, displaying statistically significant differences (P<0.05). Compared with model group and inhibitor group, imatinib group had evidently lower average optical density of Bax positive expression and notably elevated average optical density of Bcl-2 positive expression, and the differences were statistically significant (P<0.05).

Figure 1.

Expression of Bax and Bcl-2 detected via immunohistochemistry (×200). Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma-2.

Figure 2.

Average optical density value of positive expression in each group. *P<0.05 vs. sham operation group; #P<0.05 vs. model group and inhibitor group.

Results of western blotting

As shown in Fig. 3, the protein expression levels of Nrf2 and HO-1 were low in sham operation group and high in imatinib group. The statistical analysis showed that the relative protein expression levels of Nrf2 and HO-1 were obviously higher in the other three groups than those in sham operation group, with statistically significant differences (P<0.05), and they were remarkably increased in imatinib group compared with those in model group and inhibitor group, demonstrating statistically significant differences (P<0.05) (Fig. 4).

Figure 3.

Relevant protein expression determined through western blotting.

Figure 4.

Relative expression levels of relevant proteins. *P<0.05 vs. sham operation group; #P<0.05 vs. model group and inhibitor group.

Relevant mRNA expression levels measured by qPCR

As shown in Fig. 5, the relative mRNA expression levels of Nrf2 and HO-1 were distinctly upregulated in the other three groups compared with those in sham operation group, displaying statistically significant differences (P<0.05), and they were markedly raised in imatinib group compared with those in model group and inhibitor group, showing statistically significant differences (P<0.05).

Figure 5.

Relative mRNA expression levels in each group. *P<0.05 vs. sham operation group; #P<0.05 vs. model group and inhibitor group.

Results of TUNEL apoptosis assay

The apoptosis rate was prominently higher in the other three groups than that in sham operation group, and the difference was statistically significant (P<0.05), while it declined markedly in imatinib group compared with that in model group and inhibitor group, with a statistically significant difference (P<0.05) (Fig. 6).

Figure 6.

Apoptosis rate in each group. *P<0.05 vs. sham operation group; #P<0.05 vs. model group and inhibitor group.

Results of ELISA

Compared with that in sham operation group, the levels of TNF-α, IL-6, ROS and SOD were evidently increased in the other three groups, and the differences were statistically significant (P<0.05). Moreover, imatinib group had clearly reduced content of TNF-α, IL-6 and ROS and obviously raised SOD content compared with model group and inhibitor group, and the differences were statistically significant (P<0.05) (Fig. 7).

Figure 7.

Expression level of relevant factors in each group. *P<0.05 vs. sham operation group; #P<0.05 vs. model group and inhibitor group.

Discussion

Investigating the pathological responses and related mechanisms of SCI is the focus of SCI-related research fields, and further clarifying the pathological responses and related mechanisms of SCI is crucial for the effective treatment of SCI. Oxidative stress response, one of the severe pathological responses of SCI, has important effects on microenvironment, inflammation, apoptosis and necrosis following SCI (3,8,9). Moreover, it is a ‘double-edged sword’ for damaged tissues. In the case of injury, low-level oxidative stress response in the early stage can protect the tissues and cells in the injury to some extent (10,11). However, the sustained and high-level oxidative stress response will aggravate inflammation, apoptosis and necrosis, which is not conducive to tissue repair after injury (12,13). Furthermore, oxidative stress response is caused by physical and chemical factors triggered by injuries, in which ROS produced and released in quantity leads to the oxidation and biochemical changes of proteins, fats and DNA, further affecting their functions. Under the action of the ROS, the large amount of unsaturated fatty acids contained in the spinal cord tissues produces massive ROS, altering the microenvironment after SCI (14,15). Moreover, the ability of neurons to resist oxidative stress response is limited. Neurons will be damaged, apoptotic and necrotic when the accumulation of ROS and other reactive oxygen metabolites in the surrounding microenvironment exceeds the tolerance of neurons. The results of this study further proved that in the case of SCI, the content of ROS, IL-6 and TNF-α in the spinal cord tissues was increased and apoptosis was also aggravated significantly, implying that severe oxidative stress response and apoptosis are detected in the affected region after SCI.

The Nrf2/HO-1 signaling pathway is an important cell signal transduction pathway, which has been confirmed to be closely correlated with oxidative stress response and able to exert a good anti-oxidative stress response function after activated (16). Under physiological conditions, Nrf2, a key component in this pathway, is inactivated since it binds to the Keap1 protein in the cytoplasm and thus cannot be transferred into the nucleus (17). After injury occurs, the normal relation between Nrf2 and Keap1 protein is destroyed by many cytokines and physicochemical factors such as inflammatory factors, and Nrf2 is thereby free and can enter the nucleus and bind to antioxidant elements, resulting in the production and release of antioxidants (SOD and HO-1) to serve as an antioxidant (18,19). Therefore, the Nrf2/HO-1 signaling pathway is deemed as an important signaling pathway for studies of oxidative stress response after SCI. The results of this study further manifested that after SCI, the protein expression levels of Nrf2 and HO-1 as well as the SOD content in the injured spinal cord tissues were notably raised, indicating that the Nrf2/HO-1 signaling pathway in the injured local spinal cord tissues is activated after SCI, and partially plays an anti-oxidative stress role.

A previous study confirmed that imatinib has a good protective effect against traumatic cerebral hemorrhage and brain injury, and remarkably improves inflammation and neurological recovery after injury (20). It was further revealed in the results of this study that imatinib effectively inhibited oxidative stress after SCI, and thus exerted anti-inflammatory and anti-apoptotic effects. At the same time, imatinib significantly upregulated the protein expression levels of Nrf2 and HO-1 in local spinal cord tissues after SCI and increased the production and release of SOD with an anti-oxidative stress effect, while its anti-oxidative stress, anti-inflammatory and anti-apoptotic effects were blocked by Nrf2 inhibitor, suggesting that imatinib may play a role by regulating the Nrf2/HO-1 signaling pathway.

In conclusion, imatinib activates the Nrf2/HO-1 signaling pathway to suppress oxidative stress in SCI rats, thus inhibiting apoptosis and inflammation.