| Literature DB >> 31891859 |
Letícia Scribel1, Alexandre P Zavascki2, Douglas Matos3, Francine Silveira3, Talitha Peralta3, Natalia Gonçalves Landgraf3, Priscila Lamb Wink4, Anne Caroline Cezimbra da Silva5, Nadine Bordin Andriguetti6, Letícia Loss Lisboa5, Marina Venzon Antunes6, Rafael Linden6.
Abstract
This study aims to develop a liquid chromatography tandem-mass spectrometry (LC-MS/MS) method for vancomycin and creatinine measurement in dried blood spots (DBS) and to evaluate its clinical application. The analytes were extracted from DBS and analyzed by LC-MS/MS. Vancomycin and creatinine DBS and plasma concentrations were compared in 54 and 35 samples, respectively, from 29 patients. Accuracy was 94.4-102.6%, intra-assay precision was 2.1-5.6%, and inter-assay precision was 3.5-7.0%. Patients vancomycin plasma to DBS concentration ratios were highly variable (1.148-5.022), differently from creatinine (0.800-1.283). The assay has adequate analytical performance. Plasma concentrations can be satisfactorily predicted from DBS measurements for creatinine, but not for vancomycin, which limits its clinical application.Entities:
Keywords: Creatinine; Dried blood spots; Liquid chromatography-mass spectrometry; Therapeutic drug monitoring; Vancomycin
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Year: 2019 PMID： 31891859 DOI： 10.1016/j.jchromb.2019.121897
Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205