| Literature DB >> 31887870 |
Carolina Mirtes Melo1, Jéssica Ferreira Cardoso2, Fernanda Barçante Perassoli3, Ari Soares de Oliveira Neto4, Luccas Moreira Pinto5, Maria Betânia de Freitas Marques6, Wagner da Nova Mussel7, Juliana Teixeira Magalhães8, Sandra Aparecida de Lima Moura9, Marcelo Gonzaga de Freitas Araújo10, Gisele Rodrigues Da Silva11.
Abstract
The treatment of vulvovaginal candidiasis (VVC) is based on oral and vaginal formulations which show limited effectiveness. In this study, amphotericin B-loaded Eudragit RL100 nanoparticles coated with hyaluronic acid (AMP EUD nanoparticles/HA) were developed to overcome the drawbacks of the conventional formulations. AMP EUD nanoparticles/HA were synthesized by nanoprecipitation, formulated by statistical experimental design, and characterized. AMP release from EUD nanoparticles/HA and its antifungal activity in a murine model of VVC were evaluated. Nanoparticles showed 147.6 ± 16.7 nm of diameter, 0.301 ± 0.09 of polydispersity index, - 29.9 ± 3.76 mV of zeta potential, and 87.27 % of encapsulation efficiency. They released about 81 % of AMP in 96 h; and provided the elimination of 100 % of the vaginal fungal burden in 24 h. It was suggested that the AMP EUD nanoparticles/HA penetrated into the vaginal epithelium via CD44 receptors. These AMP EUD nanoparticles/HA represent a non-conventional vaginal formulation to improve the treatment of VVC.Entities:
Keywords: Amphotericin B; Amphotericin B-loaded Eudragit RL100 nanoparticles coated with Hyaluronic Acid; Chloramphenicol; Chloroform; Copolymer of poly(ethylacrylate, methyl-methacrylate, and chloro trimethyl-ammonioethyl methacrylate); Cyclophosphamide; Dibasic sodium phosphate dodecahydrate; Estradiol cypionate; Eudragit RL100; Hyaluronic acid; Hyaluronic acid sodium salt; Methanol; Phosphoric acid; Polyoxyethylenesorbitan monooleate; Potassium phosphate monobasic; Sodium hydroxide; Vulvovaginal Candidiasis
Year: 2019 PMID： 31887870 DOI： 10.1016/j.carbpol.2019.115608
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381