Z N Yu1, J Wang2, H Ho3, Y T Wang4, S N Huang2, R W Han5. 1. Haidu College(.)Qingdao Agricultural University, Laiyang 265200, Shandong, China. 2. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, Shandong, China. 3. Auckland Bioengineering Institute, The University of Auckland, Auckland, New Zealand. 4. Institute of Quality Standard and Test Technology for Agro-products, Shandong Academy of Agricultural Sciences, Jinan 250100, China. 5. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, Shandong, China. Electronic address: qauhan@qq.com.
Abstract
OBJECTIVES: The objective was to find the differences in the prevalence and resistance of Escherischia coli isolated from raw milk samples from mastitis cases in four regions of China. METHODS: A total of 750 bovine raw milk samples from mastitis cases were collected from four regions of China over two seasons. Antimicrobial resistance against 29 antimicrobial agents was determined, and 27 drug-resistant genes were tested. RESULTS: Eighty-three strains (11.1%) of E. coli were isolated and identified. No significant differences in the number of E. coli isolates were observed between the two sampling seasons in the same regions (P>0.05). However, a significant difference in E. coli prevalence was found among the four different regions (P<0.01). The isolates were most frequently resistant to penicillin (100%), acetylspiramycin (100%), lincomycin (98.8%), oxacillin (98.8%) and sulphamethoxazole (53%). All the E. coli strains were multiresistant to at least three antimicrobial classes, and the most frequent multidrug-resistance patterns for the isolates were resistant to three (36.1%) or four (39.8%) classes of drugs simultaneously. The blaTEM gene (n=69; 83.1%) was the most frequently detected resistance gene. The most frequent gene combinations were a four-gene pattern of blaCTX-M-sulII-blaTEM-sulI (n=13; 15.7%) and a three-gene pattern of blaCTX-M-aph (3)-II-blaTEM (n=11; 13.3%). CONCLUSIONS: This study indicated that there is a high incidence of E. coli with a great variation in resistance patterns and resistance genes; this is a matter of great concern for public and animal health in China.
OBJECTIVES: The objective was to find the differences in the prevalence and resistance of Escherischia coli isolated from raw milk samples from mastitis cases in four regions of China. METHODS: A total of 750 bovine raw milk samples from mastitis cases were collected from four regions of China over two seasons. Antimicrobial resistance against 29 antimicrobial agents was determined, and 27 drug-resistant genes were tested. RESULTS: Eighty-three strains (11.1%) of E. coli were isolated and identified. No significant differences in the number of E. coli isolates were observed between the two sampling seasons in the same regions (P>0.05). However, a significant difference in E. coli prevalence was found among the four different regions (P<0.01). The isolates were most frequently resistant to penicillin (100%), acetylspiramycin (100%), lincomycin (98.8%), oxacillin (98.8%) and sulphamethoxazole (53%). All the E. coli strains were multiresistant to at least three antimicrobial classes, and the most frequent multidrug-resistance patterns for the isolates were resistant to three (36.1%) or four (39.8%) classes of drugs simultaneously. The blaTEM gene (n=69; 83.1%) was the most frequently detected resistance gene. The most frequent gene combinations were a four-gene pattern of blaCTX-M-sulII-blaTEM-sulI (n=13; 15.7%) and a three-gene pattern of blaCTX-M-aph (3)-II-blaTEM (n=11; 13.3%). CONCLUSIONS: This study indicated that there is a high incidence of E. coli with a great variation in resistance patterns and resistance genes; this is a matter of great concern for public and animal health in China.
Authors: Valentina Dazio; Aurélien Nigg; Janne S Schmidt; Michael Brilhante; Nico Mauri; Stephan P Kuster; Stefanie Gobeli Brawand; Gertraud Schüpbach-Regula; Barbara Willi; Andrea Endimiani; Vincent Perreten; Simone Schuller Journal: J Vet Intern Med Date: 2021-02-01 Impact factor: 3.333
Authors: Ghada Elderdiri Abdelwahab; Hassan Zackaria Ali Ishag; Zulaikha Mohamed Al Hammadi; Saeed Mohamed S Al Yammahi; Mohd Faoruk Bin Mohd Yusof; Muna Sayed Y Al Yassi; Shaikha Saeed A Al Neyadi; Asma Mohammed A Al Mansoori; Fawzia Hassan A Al Hamadi; Ibtesam Abdullah S Al Hamadi; Mohamed Ali Abdalla Al Hosani; Salama Suhail Mohammed Al Muhairi Journal: Int J Microbiol Date: 2022-04-26