Yong Hyun Jun1, Gang San Ju2, Yoon Young Chung1, Hye-Kyoung Shin1, Dong-Joon Kim3, Min Seon Choi4, Seong Taeck Kim5, Kyung Min Son6. 1. Department of Anatomy, School of Medicine, Chosun University, Gwang-ju, Republic of Korea. 2. Department of Plastic and Reconstructive Surgery, Chosun University Hospital, Gwang-ju, Republic of Korea. 3. Department of Anesthesiology and Pain Medicine, Chosun University Hospital, Gwang-ju, Republic of Korea. 4. Department of Pediatrics, Chosun University Hospital, Gwang-ju, Republic of Korea. 5. Department of Ophthalmology, Chosun University Hospital, Gwang-ju, Republic of Korea. 6. Department of Plastic and Reconstructive Surgery, Chosun University Hospital, Gwang-ju, Republic of Korea jyh1483@naver.com.
Abstract
BACKGROUND/AIM: Vascular endothelial growth factor (VEGF) provides tolerance against ischemic brain injury, yet, the pattern of VEGF expression in the neurogenic zones following chronic cerebral hypoperfusion has not been studied. Here we evaluated the immunoreactivity of VEGF in a rat model of chronic cerebral hypoperfusion. MATERIALS AND METHODS: Chronic hypoperfusion was induced by bilateral common carotid artery ligation in rats. Immunohistochemistry was performed against hypoxia-inducible factor-1α (HIF-1α) and VEGF on brain sections. RESULTS: The density of HIF1α-positive cells in the hypoxia group was increased in the cerebral cortex and hippocampus. Further, the density of VEGF-positive cells was significantly higher in the hypoxia group compared to the control group in the cerebral cortex whereas it was similar in the subventricular zone, and in the dentate gyrus in the hippocampus between the two groups. CONCLUSION: The pattern of VEGF expression varies in different brain regions following chronic cerebral hypoperfusion. Copyright
BACKGROUND/AIM: Vascular endothelial growth factor (VEGF) provides tolerance against ischemic brain injury, yet, the pattern of VEGF expression in the neurogenic zones following chronic cerebral hypoperfusion has not been studied. Here we evaluated the immunoreactivity of VEGF in a rat model of chronic cerebral hypoperfusion. MATERIALS AND METHODS: Chronic hypoperfusion was induced by bilateral common carotid artery ligation in rats. Immunohistochemistry was performed against hypoxia-inducible factor-1α (HIF-1α) and VEGF on brain sections. RESULTS: The density of HIF1α-positive cells in the hypoxia group was increased in the cerebral cortex and hippocampus. Further, the density of VEGF-positive cells was significantly higher in the hypoxia group compared to the control group in the cerebral cortex whereas it was similar in the subventricular zone, and in the dentate gyrus in the hippocampus between the two groups. CONCLUSION: The pattern of VEGF expression varies in different brain regions following chronic cerebral hypoperfusion. Copyright
Authors: David Kung-Chun Chiu; Aki Pui-Wah Tse; Iris Ming-Jing Xu; Jane Di Cui; Robin Kit-Ho Lai; Lynna Lan Li; Hui-Yu Koh; Felice Ho-Ching Tsang; Larry Lai Wei; Chun-Ming Wong; Irene Oi-Lin Ng; Carmen Chak-Lui Wong Journal: Nat Commun Date: 2017-09-11 Impact factor: 14.919