Hirotake Kusumoto1, Kotaro Tashiro1, Syunji Shimaoka1, Koichiro Tsukasa1, Yukiko Baba1, Saori Furukawa1, Junichiro Furukawa1, Toru Niihara2, Takaaki Hirotsu3,4,5, Takayuki Uozumi6. 1. Department of Gastroenterology, Nanpuh Hospital, Kagoshima, Japan. 2. Department of Gastroenterology, Nanpuh Hospital, Kagoshima, Japan uozumi@hbio.jp t.niihara@nanpuh.or.jp. 3. Hirotsu Bio Science Inc., Tokyo, Japan. 4. Department of Biology, Graduate School of Sciences, Kyushu University, Fukuoka, Japan. 5. School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia. 6. Hirotsu Bio Science Inc., Tokyo, Japan uozumi@hbio.jp t.niihara@nanpuh.or.jp.
Abstract
BACKGROUND/AIM: Early detection of gastrointestinal cancer may reduce mortality. Recently, Caenorhabditis elegans has been reported to be capable of differentiating patients with cancers from healthy persons by the smell of urine. This novel technique using C. elegans olfaction has been named as Nematode-NOSE (N-NOSE). MATERIALS AND METHODS: We collected 180 urine samples from patients with gastrointestinal cancer and 76 samples from healthy subjects. N-NOSE test was performed using these samples and N-NOSE index was obtained. Quantification of the olfactory behavior of C. elegans was performed as established in past studies. By receiver operating characteristic (ROC) analysis, we examined the diagnostic capability of N-NOSE. RESULTS: ROC analysis revealed that N-NOSE showed an area under the curve value of more than 0.80, even in early-stage cancers. CONCLUSION: C. elegans olfaction enabled the detection of gastrointestinal cancers from urine with high sensitivity, which can provide the basis for the development of N-NOSE as a gastrointestinal cancer screening test. Copyright
BACKGROUND/AIM: Early detection of gastrointestinal cancer may reduce mortality. Recently, Caenorhabditis elegans has been reported to be capable of differentiating patients with cancers from healthy persons by the smell of urine. This novel technique using C. elegans olfaction has been named as Nematode-NOSE (N-NOSE). MATERIALS AND METHODS: We collected 180 urine samples from patients with gastrointestinal cancer and 76 samples from healthy subjects. N-NOSE test was performed using these samples and N-NOSE index was obtained. Quantification of the olfactory behavior of C. elegans was performed as established in past studies. By receiver operating characteristic (ROC) analysis, we examined the diagnostic capability of N-NOSE. RESULTS: ROC analysis revealed that N-NOSE showed an area under the curve value of more than 0.80, even in early-stage cancers. CONCLUSION:C. elegans olfaction enabled the detection of gastrointestinal cancers from urine with high sensitivity, which can provide the basis for the development of N-NOSE as a gastrointestinal cancer screening test. Copyright
Authors: M S Pepe; R Etzioni; Z Feng; J D Potter; M L Thompson; M Thornquist; M Winget; Y Yasui Journal: J Natl Cancer Inst Date: 2001-07-18 Impact factor: 13.506
Authors: Esther M Stoop; Margriet C de Haan; Thomas R de Wijkerslooth; Patrick M Bossuyt; Marjolein van Ballegooijen; C Yung Nio; Marc J van de Vijver; Katharina Biermann; Maarten Thomeer; Monique E van Leerdam; Paul Fockens; Jaap Stoker; Ernst J Kuipers; Evelien Dekker Journal: Lancet Oncol Date: 2011-11-15 Impact factor: 41.316