Masayoshi Kobayashi1, Nagahide Matsubara2, Yutaka Nakachi3, Yasushi Okazaki3, Motoi Uchino4, Hiroki Ikeuchi4, Jihyng Song1, Kei Kimura1, Michiko Yasuhara1, Akihito Babaya1, Tomoki Yamano1, Masataka Ikeda1, Hiroki Nishikawa5, Ikuo Matsuda6, Seiichi Hirota6, Naohiro Tomita1. 1. Division of Lower Gastrointestinal Surgery, Department of Surgery, Hyogo College of Medicine, Nishinomiya, Japan. 2. Division of Lower Gastrointestinal Surgery, Department of Surgery, Hyogo College of Medicine, Nishinomiya, Japan nagamb@hyo-med.ac.jp. 3. Division of Translational Research, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Japan. 4. Department of Inflammatory Bowel Disease, Division of Surgery, Hyogo College of Medicine, Nishinomiya, Japan. 5. Center for Clinical Research and Education, Hyogo College of Medicine, Nishinomiya, Japan. 6. Department of Surgical Pathology, Hyogo College of Medicine, Nishinomiya, Japan.
Abstract
BACKGROUND/AIM: The difficulty of early diagnosis of colitis associated colorectal cancer (CACRC) due to colonic mucosal changes in long-standing ulcerative colitis (UC) patients is often experienced in daily clinical practice. Noninvasive objective monitoring for cancer development is advantageous for optimizing treatment strategies in UC patients. We aimed to examine the epigenetic alterations occurring in CACRC, focusing on DNA hypermethylation of CpG islands. MATERIALS AND METHODS: The level of DNA methylation in CpG cites was compared between CACRC and the counterpart non-tumorous mucosa using Infinium HumanMethylation 450K BeadChip. RESULTS: Our subjects included 3 males and 3 females (median age, 49.5 years). The 450K CpG site DNA methylation microarray revealed that the difference in β value (level of hypermethylation) was the highest for corcicotropin releasing hormone receptor 2 (CRHR2) between CACRC and counterpart non-tumorous mucosa. CONCLUSION: Detection of hypermethylation of CRHR2 may be promising for cancer screening in UC patients. Copyright
BACKGROUND/AIM: The difficulty of early diagnosis of colitis associated colorectal cancer (CACRC) due to colonic mucosal changes in long-standing ulcerative colitis (UC) patients is often experienced in daily clinical practice. Noninvasive objective monitoring for cancer development is advantageous for optimizing treatment strategies in UCpatients. We aimed to examine the epigenetic alterations occurring in CACRC, focusing on DNA hypermethylation of CpG islands. MATERIALS AND METHODS: The level of DNA methylation in CpG cites was compared between CACRC and the counterpart non-tumorous mucosa using Infinium HumanMethylation 450K BeadChip. RESULTS: Our subjects included 3 males and 3 females (median age, 49.5 years). The 450K CpG site DNA methylation microarray revealed that the difference in β value (level of hypermethylation) was the highest for corcicotropin releasing hormone receptor 2 (CRHR2) between CACRC and counterpart non-tumorous mucosa. CONCLUSION: Detection of hypermethylation of CRHR2 may be promising for cancer screening in UCpatients. Copyright
Authors: Hans Raskov; Hans-Christian Pommergaard; Jakob Burcharth; Jacob Rosenberg Journal: World J Gastroenterol Date: 2014-12-28 Impact factor: 5.742