Somayeh Hajipour1, Yaghoob Farbood2, Mohammad Kazem Gharib-Naseri1, Gholamreza Goudarzi3, Mohammad Rashno4, Heidar Maleki5, Nima Bakhtiari6, Ali Nesari1, Seyed Esmaeil Khoshnam1, Mahin Dianat2, Behjat Sarkaki1, Alireza Sarkaki7. 1. Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 2. Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Physiology, Medicine Faculty, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 3. Air Pollution and Respiratory Diseases (APRD) Research Center, Environmental Technologies Research Center (ETRC), Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 4. Department of Immunology, Medicine Faculty, Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 5. Air Pollution and Respiratory Diseases (APRD) Research Center, Environmental Technologies Research Center (ETRC), Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Environmental Engineer, Faculty of Water Sciences Engineering, Shahid Chamran University of Ahvaz, Ahvaz, Iran. 6. Pain Research Center, Imam Khomeiny Hospital Research and Development Unit, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 7. Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Iran National Science Foundation (INSF), Science Deputy of Presidency, Islamic Republic of Iran, Iran; Department of Physiology, Medicine Faculty, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Medicinal Plant Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: sarkaki_a@ajums.ac.ir.
Abstract
OBJECTIVES: Exposure of healthy subjects to ambient airborne dusty particulate matter (PM) causes brain dysfunction. This study aimed to investigate the effect of sub-chronic inhalation of ambient PM in a designed special chamber to create factual dust storm (DS) conditions on spatial cognition, hippocampal long-term potentiation (LTP), inflammatory cytokines, and oxidative stress in the brain tissue. METHODS: Adult male Wistar rats (250-300 g) were randomly divided into four groups: Sham (clean air, the concentration of dusty PM was <150 μg/m3), DS1 (200-500 μg/m3), DS2 (500-2000 μg/m3) and DS3 (2000-8000 μg/m3). Experimental rats were exposed to clean air or different sizes and concentrations of dust PM storm for four consecutive weeks (exposure was during 1-4, 8-11, 15-16 and 20-23 days, 30 min, twice daily) in a real-ambient dust exposure chamber. Subsequently, cognitive performance, hippocampal LTP, blood-brain barrier (BBB) permeability and brain edema of the animals evaluated. As well as, inflammatory cytokines and oxidative stress indexes in the brain tissue measured using ELISA assays. RESULTS: Exposing to dust PM impaired spatial memory (p < 0.001), hippocampal LTP (p < 0.001). These disturbances were in line with the severe damage to respiratory system followed by disruption of BBB integrity (p < 0.001), increased brain edema (p < 0.001), inflammatory cytokines (p < 0.001) excretion and oxidative stress (p < 0.001) in brain tissue. CONCLUSIONS: Our study showed that exposure to ambient dust PM increased brain edema and BBB permeability, induced memory impairment and hippocampal LTP deficiency by increasing the inflammatory responses and oxidative stress in the brain of the rats.
OBJECTIVES: Exposure of healthy subjects to ambient airborne dusty particulate matter (PM) causes brain dysfunction. This study aimed to investigate the effect of sub-chronic inhalation of ambient PM in a designed special chamber to create factual dust storm (DS) conditions on spatial cognition, hippocampal long-term potentiation (LTP), inflammatory cytokines, and oxidative stress in the brain tissue. METHODS: Adult male Wistar rats (250-300 g) were randomly divided into four groups: Sham (clean air, the concentration of dusty PM was <150 μg/m3), DS1 (200-500 μg/m3), DS2 (500-2000 μg/m3) and DS3 (2000-8000 μg/m3). Experimental rats were exposed to clean air or different sizes and concentrations of dust PM storm for four consecutive weeks (exposure was during 1-4, 8-11, 15-16 and 20-23 days, 30 min, twice daily) in a real-ambient dust exposure chamber. Subsequently, cognitive performance, hippocampal LTP, blood-brain barrier (BBB) permeability and brain edema of the animals evaluated. As well as, inflammatory cytokines and oxidative stress indexes in the brain tissue measured using ELISA assays. RESULTS: Exposing to dust PM impaired spatial memory (p < 0.001), hippocampal LTP (p < 0.001). These disturbances were in line with the severe damage to respiratory system followed by disruption of BBB integrity (p < 0.001), increased brain edema (p < 0.001), inflammatory cytokines (p < 0.001) excretion and oxidative stress (p < 0.001) in brain tissue. CONCLUSIONS: Our study showed that exposure to ambient dust PM increased brain edema and BBB permeability, induced memory impairment and hippocampal LTP deficiency by increasing the inflammatory responses and oxidative stress in the brain of the rats.
Authors: Matthew J Mears; David M Aslaner; Chad T Barson; Mitchell D Cohen; Matthew W Gorr; Loren E Wold Journal: Life Sci Date: 2021-11-14 Impact factor: 5.037
Authors: Omar Hahad; Jos Lelieveld; Frank Birklein; Klaus Lieb; Andreas Daiber; Thomas Münzel Journal: Int J Mol Sci Date: 2020-06-17 Impact factor: 5.923
Authors: So Young Kim; Kyung Woon Kim; So Min Lee; Da-Hye Lee; Sohyeon Park; Bu Soon Son; Moo Kyun Park Journal: Oxid Med Cell Longev Date: 2020-12-28 Impact factor: 6.543