| Literature DB >> 31873214 |
Miguel A Pleitez1,2, Asrar Ali Khan3,4,5,6, Alice Soldà7,8, Andriy Chmyrov7,8, Josefine Reber7,8, Francesca Gasparin7,8, Markus R Seeger7,8, Benedikt Schätz7,8, Stephan Herzig3,4,5,6, Marcel Scheideler3,4,5,6, Vasilis Ntziachristos9,10.
Abstract
We develop mid-infrared optoacoustic microscopy (MiROM) for label-free, bond-selective, live-cell metabolic imaging, enabling spatiotemporal monitoring of carbohydrates, lipids and proteins in cells and tissues. Using acoustic detection of optical absorption, MiROM converts mid-infrared sensing into a positive-contrast imaging modality with negligible photodamage and high sensitivity. We use MiROM to observe changes in intrinsic carbohydrate distribution from a diffusive spatial pattern to tight co-localization with lipid droplets during adipogenesis.Entities:
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Year: 2019 PMID： 31873214 DOI： 10.1038/s41587-019-0359-9
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908