Literature DB >> 31872861

Comparison Between Integrated Genomic DNA/RNA Profiling and Fluorescence In Situ Hybridization in the Detection of MYC, BCL-2, and BCL-6 Gene Rearrangements in Large B-Cell Lymphomas.

Daniel P Cassidy1, Jennifer R Chapman1, Rafael Lopez1, Kyle White1, Yao-Shan Fan1, Carmen Casas1, Eric A Severson2, Francisco Vega3.   

Abstract

OBJECTIVES: To compare fluorescence in situ hybridization (FISH) and a commercially available sequencing assay for comprehensive genomic profiling (CGP) to determine the best approach to identify gene rearrangements (GRs) in large B-cell lymphomas (LBCLs).
METHODS: Comparison of standard-of-care FISH assays (including a two-probe approach for MYC; break-apart and fusion probes) and an integrated genomic DNA/RNA sequencing CGP approach on a set of 69 consecutive LBCL cases.
RESULTS: CGP detected GRs, including those involving MYC (1), BCL-2 (3), and BCL-6 (3), not detected by FISH. FISH detected non-IgH-MYC (4) and BCL-6 (2) GRs that were not detected by CGP. In four instances, standalone CGP or FISH testing would have missed a double-hit lymphoma.
CONCLUSIONS: CGP was superior to FISH in the detection of IgH-MYC rearrangements but was inferior for the detection of non-IgH-MYC rearrangements. Our study demonstrates the rationale for development of a customized approach to identify GRs in LBCLs. © American Society for Clinical Pathology, 2019. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Entities:  

Keywords:  zzm321990 MYCzzm321990 ; Comprehensive genomic profiling; FISH; Gene rearrangements; High-grade B-cell lymphoma; Prognosis; Sequencing

Mesh：

Substances：

Year:  2020        PMID： 31872861      PMCID： PMC7007318          DOI： 10.1093/ajcp/aqz172

Source DB:  PubMed          Journal:  Am J Clin Pathol        ISSN： 0002-9173            Impact factor:   2.493


  17 in total

Review 1.  Approach to the diagnosis and treatment of high-grade B-cell lymphomas with MYC and BCL2 and/or BCL6 rearrangements.

Authors:  Pierre Sesques; Nathalie A Johnson
Journal:  Blood       Date:  2016-11-07       Impact factor: 22.113

Review 2.  Activating somatic mutations in diffuse large B-cell lymphomas: lessons from next generation sequencing and key elements in the precision medicine era.

Authors:  Elodie Bohers; Sylvain Mareschal; Philippe Bertrand; Pierre Julien Viailly; Sydney Dubois; Catherine Maingonnat; Philippe Ruminy; Hervé Tilly; Fabrice Jardin
Journal:  Leuk Lymphoma       Date:  2014-10-09

3.  Integrated genomic DNA/RNA profiling of hematologic malignancies in the clinical setting.

Authors:  Jie He; Omar Abdel-Wahab; Michelle K Nahas; Kai Wang; Raajit K Rampal; Andrew M Intlekofer; Jay Patel; Andrei Krivstov; Garrett M Frampton; Lauren E Young; Shan Zhong; Mark Bailey; Jared R White; Steven Roels; Jason Deffenbaugh; Alex Fichtenholtz; Timothy Brennan; Mark Rosenzweig; Kimberly Pelak; Kristina M Knapp; Kristina W Brennan; Amy L Donahue; Geneva Young; Lazaro Garcia; Selmira T Beckstrom; Mandy Zhao; Emily White; Vera Banning; Jamie Buell; Kiel Iwanik; Jeffrey S Ross; Deborah Morosini; Anas Younes; Alan M Hanash; Elisabeth Paietta; Kathryn Roberts; Charles Mullighan; Ahmet Dogan; Scott A Armstrong; Tariq Mughal; Jo-Anne Vergilio; Elaine Labrecque; Rachel Erlich; Christine Vietz; Roman Yelensky; Philip J Stephens; Vincent A Miller; Marcel R M van den Brink; Geoff A Otto; Doron Lipson; Ross L Levine
Journal:  Blood       Date:  2016-03-10       Impact factor: 22.113

4.  Novel FISH probes designed to detect IGK-MYC and IGL-MYC rearrangements in B-cell lineage malignancy identify a new breakpoint cluster region designated BVR2.

Authors:  R R Einerson; M E Law; H E Blair; P J Kurtin; R F McClure; R P Ketterling; H C Flynn; A Dogan; E D Remstein
Journal:  Leukemia       Date:  2006-08-03       Impact factor: 11.528

5.  Lymphomas with concurrent BCL2 and MYC translocations: the critical factors associated with survival.

Authors:  Nathalie A Johnson; Kerry J Savage; Olga Ludkovski; Susana Ben-Neriah; Ryan Woods; Christian Steidl; Martin J S Dyer; Reiner Siebert; John Kuruvilla; Richard Klasa; Joseph M Connors; Randy D Gascoyne; Douglas E Horsman
Journal:  Blood       Date:  2009-07-13       Impact factor: 22.113

6.  Variable breakpoints in Burkitt lymphoma cells with chromosomal t(8;14) translocation separate c-myc and the IgH locus up to several hundred kb.

Authors:  S Joos; M H Falk; P Lichter; F G Haluska; B Henglein; G M Lenoir; G W Bornkamm
Journal:  Hum Mol Genet       Date:  1992-11       Impact factor: 6.150

7.  High-resolution architecture and partner genes of MYC rearrangements in lymphoma with DLBCL morphology.

Authors:  Lauren C Chong; Susana Ben-Neriah; Graham W Slack; Ciara Freeman; Daisuke Ennishi; Anja Mottok; Brett Collinge; Pau Abrisqueta; Pedro Farinha; Merrill Boyle; Barbara Meissner; Robert Kridel; Alina S Gerrie; Diego Villa; Kerry J Savage; Laurie H Sehn; Reiner Siebert; Ryan D Morin; Randy D Gascoyne; Marco A Marra; Joseph M Connors; Andrew J Mungall; Christian Steidl; David W Scott
Journal:  Blood Adv       Date:  2018-10-23

8.  Clinical Evaluation of Massively Parallel RNA Sequencing for Detecting Recurrent Gene Fusions in Hematologic Malignancies.

Authors:  Borahm Kim; Hyeonah Lee; Saeam Shin; Seung-Tae Lee; Jong Rak Choi
Journal:  J Mol Diagn       Date:  2018-10-19       Impact factor: 5.568

9.  Molecular subtypes of diffuse large B cell lymphoma are associated with distinct pathogenic mechanisms and outcomes.

Authors:  Bjoern Chapuy; Chip Stewart; Andrew J Dunford; Jaegil Kim; Atanas Kamburov; Robert A Redd; Mike S Lawrence; Margaretha G M Roemer; Amy J Li; Marita Ziepert; Annette M Staiger; Jeremiah A Wala; Matthew D Ducar; Ignaty Leshchiner; Ester Rheinbay; Amaro Taylor-Weiner; Caroline A Coughlin; Julian M Hess; Chandra S Pedamallu; Dimitri Livitz; Daniel Rosebrock; Mara Rosenberg; Adam A Tracy; Heike Horn; Paul van Hummelen; Andrew L Feldman; Brian K Link; Anne J Novak; James R Cerhan; Thomas M Habermann; Reiner Siebert; Andreas Rosenwald; Aaron R Thorner; Matthew L Meyerson; Todd R Golub; Rameen Beroukhim; Gerald G Wulf; German Ott; Scott J Rodig; Stefano Monti; Donna S Neuberg; Markus Loeffler; Michael Pfreundschuh; Lorenz Trümper; Gad Getz; Margaret A Shipp
Journal:  Nat Med       Date:  2018-04-30       Impact factor: 53.440

10.  Genetics and Pathogenesis of Diffuse Large B-Cell Lymphoma.

Authors:  Roland Schmitz; George W Wright; Da Wei Huang; Calvin A Johnson; James D Phelan; James Q Wang; Sandrine Roulland; Monica Kasbekar; Ryan M Young; Arthur L Shaffer; Daniel J Hodson; Wenming Xiao; Xin Yu; Yandan Yang; Hong Zhao; Weihong Xu; Xuelu Liu; Bin Zhou; Wei Du; Wing C Chan; Elaine S Jaffe; Randy D Gascoyne; Joseph M Connors; Elias Campo; Armando Lopez-Guillermo; Andreas Rosenwald; German Ott; Jan Delabie; Lisa M Rimsza; Kevin Tay Kuang Wei; Andrew D Zelenetz; John P Leonard; Nancy L Bartlett; Bao Tran; Jyoti Shetty; Yongmei Zhao; Dan R Soppet; Stefania Pittaluga; Wyndham H Wilson; Louis M Staudt
Journal:  N Engl J Med       Date:  2018-04-12       Impact factor: 91.245
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  2 in total

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2.  Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing.

Authors:  Mark Pieterse; Joost Swennenhuis; G Tjitske Los-de Vries; Amin Allahyar; Mehmet Yilmaz; Roos Leguit; Ruud W J Meijers; Robert van der Geize; Joost Vermaat; Arjen Cleven; Tom van Wezel; Arjan Diepstra; Léon C van Kempen; Nathalie J Hijmering; Phylicia Stathi; Milan Sharma; Adrien S J Melquiond; Paula J P de Vree; Marjon J A M Verstegen; Peter H L Krijger; Karima Hajo; Marieke Simonis; Agata Rakszewska; Max van Min; Daphne de Jong; Bauke Ylstra; Harma Feitsma; Erik Splinter; Wouter de Laat
Journal:  Nat Commun       Date:  2021-06-07       Impact factor: 14.919
  2 in total

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