Junyoung Oh1, Dong Hyun Kim2,3, Gi-Young Kim1, Eun-Jin Park4, Jong Hoon Ryu5,6, Ji Wook Jung7, Se Jin Park8, Gwang-Woo Kim9, Seungheon Lee10. 1. Department of Marine Life Science, College of Ocean Science, Jeju National University, Jeju, 63243, Republic of Korea. 2. Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 604-714, Republic of Korea. 3. Department of Food Bioengineering, Jeju National University, Jeju, 63243, Republic of Korea. 4. Institute of Convergence Bio-Health, Dong-A University, Busan, 604-714, Republic of Korea. 5. Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Hoeki-dong, Dongdaemoon-Ku, Seoul, 130-701, Republic of Korea. 6. Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoeki-dong, Dongdaemoon-Ku, Seoul, 130-701, Republic of Korea. 7. Department of Herbal Medicinal Pharmacology, College of Herbal Bio-industry, Daegu Haany University, Yugok-dong, Gyeongsan, 712-715, Republic of Korea. 8. School of Natural Resources and Environmental Sciences, Kangwon National University, Chuncheon, 24341, Republic of Korea. 9. Marine Bio Convergence Center, Gangneung Science & Industry Promotion Agency, Gangneung, 25440, Republic of Korea. 10. Department of Marine Life Science, College of Ocean Science, Jeju National University, Jeju, 63243, Republic of Korea. slee76@jejunu.ac.kr.
Abstract
OBJECTIVE: To determine the effects of Hydrangeae Dulcis Folium (EHDF) on physical stress, changes in the whole-body cortisol level and behaviour in zebrafish (Danio rerio). METHODS: One hundred and seventy-four fish were randomly divided into 4 [adrenocorticotropin hormone (ACTH) challenge test: 4 fish per group] or 6 groups (behavioural test: 10-12 fish per group, whole-body cortisol: 4 fish per group). Net handling stress (NHS) was used to induce physical stress. Fish were treated with vehicle or EHDF (5-20 mg/L) for 6 min before they were exposed to stress. And then, fish were sacrificed for collecting body fluid from whole-body or conducted behavioural tests, including novel tank test and open field test, and were evaluated to observe anxiety-like behaviours and locomotion. In addition, to elucidate the mode of action of the anti-stress effects of EHDF, ACTH (0.2 IU/g, i.p.) challenge test was performed. RESULTS: The increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with EHDF at 5-20 mg/L (P <0.05). Moreover, compared with the unstressed group, which was not treated with NHS, the whole-body cortisol level was significantly increased by treatment with NHS (P <0.05). Compared with the NHS-treated stressed control group, pre-treatment with EHDF at concentrations of 5-20 mg/L for 6 min significantly prevented the NHS-increased whole-body cortisol level (<0.05). In addition, ACTH challenge test showed that EHDF completely blocked the effects of ACTH on cortisol secretion (P <0.05). CONCLUSION: EHDF may be a good antistress candidate and its mechanism of action may be related to its positive effects on cortisol release.
OBJECTIVE: To determine the effects of Hydrangeae Dulcis Folium (EHDF) on physical stress, changes in the whole-body cortisol level and behaviour in zebrafish (Danio rerio). METHODS: One hundred and seventy-four fish were randomly divided into 4 [adrenocorticotropin hormone (ACTH) challenge test: 4 fish per group] or 6 groups (behavioural test: 10-12 fish per group, whole-body cortisol: 4 fish per group). Net handling stress (NHS) was used to induce physical stress. Fish were treated with vehicle or EHDF (5-20 mg/L) for 6 min before they were exposed to stress. And then, fish were sacrificed for collecting body fluid from whole-body or conducted behavioural tests, including novel tank test and open field test, and were evaluated to observe anxiety-like behaviours and locomotion. In addition, to elucidate the mode of action of the anti-stress effects of EHDF, ACTH (0.2 IU/g, i.p.) challenge test was performed. RESULTS: The increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with EHDF at 5-20 mg/L (P <0.05). Moreover, compared with the unstressed group, which was not treated with NHS, the whole-body cortisol level was significantly increased by treatment with NHS (P <0.05). Compared with the NHS-treated stressed control group, pre-treatment with EHDF at concentrations of 5-20 mg/L for 6 min significantly prevented the NHS-increased whole-body cortisol level (<0.05). In addition, ACTH challenge test showed that EHDF completely blocked the effects of ACTH on cortisol secretion (P <0.05). CONCLUSION:EHDF may be a good antistress candidate and its mechanism of action may be related to its positive effects on cortisol release.
Authors: K Kamei; H Matsuoka; S I Furuhata; R I Fujisaki; T Kawakami; S Mogi; H Yoshihara; N Aoki; A Ishii; T Shibuya Journal: Acta Med Okayama Date: 2000-10 Impact factor: 0.892
Authors: Rupert J Egan; Carisa L Bergner; Peter C Hart; Jonathan M Cachat; Peter R Canavello; Marco F Elegante; Salem I Elkhayat; Brett K Bartels; Anna K Tien; David H Tien; Sopan Mohnot; Esther Beeson; Eric Glasgow; Hakima Amri; Zofia Zukowska; Allan V Kalueff Journal: Behav Brain Res Date: 2009-06-18 Impact factor: 3.332
Authors: Sebastiaan A Brittijn; Suzanne J Duivesteijn; Mounia Belmamoune; Laura F M Bertens; Wilbert Bitter; Joost D de Bruijn; Danielle L Champagne; Edwin Cuppen; Gert Flik; Christina M Vandenbroucke-Grauls; Richard A J Janssen; Ilse M L de Jong; Edo Ronald de Kloet; Alexander Kros; Annemarie H Meijer; Juriaan R Metz; Astrid M van der Sar; Marcel J M Schaaf; Stefan Schulte-Merker; Herman P Spaink; Paul P Tak; Fons J Verbeek; Margriet J Vervoordeldonk; Freek J Vonk; Frans Witte; Huipin Yuan; Michael K Richardson Journal: Int J Dev Biol Date: 2009 Impact factor: 2.203
Authors: Tracy L Keller; Davide Zocco; Mark S Sundrud; Margaret Hendrick; Maja Edenius; Jinah Yum; Yeon-Jin Kim; Hak-Kyo Lee; Joseph F Cortese; Dyann F Wirth; John David Dignam; Anjana Rao; Chang-Yeol Yeo; Ralph Mazitschek; Malcolm Whitman Journal: Nat Chem Biol Date: 2012-02-12 Impact factor: 15.040