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Literature DB >> 31867856

X-ray crystallographic structure of BshB, the zinc-dependent deacetylase involved in bacillithiol biosynthesis.

Robert L Woodward¹, Michaela M Castleman², Chelsea E Meloche², Mary E Karpen², Clare G Carlson², William H Yobi¹, Jacqueline C Jepsen¹, Benjamin W Lewis¹, Brooke N Zarnosky¹, Paul D Cook².

Abstract

Many gram-positive bacteria produce bacillithiol to aid in the maintenance of redox homeostasis and degradation of toxic compounds, including the antibiotic fosfomycin. Bacillithiol is produced via a three-enzyme pathway that includes the action of the zinc-dependent deacetylase BshB. Previous studies identified conserved aspartate and histidine residues within the active site that are involved in metal binding and catalysis, but the enzymatic mechanism is not fully understood. Here we report two X-ray crystallographic structures of BshB from Bacillus subtilis that provide insight into the BshB catalytic mechanism.

Entities: Chemical Species

Keywords: Bacillithiol; X-ray crystallography; deacetylase; gram-positive; low-molecular-weight thiol

Mesh：

Substances：

Year: 2019 PMID： 31867856 PMCID： PMC7096702 DOI： 10.1002/pro.3808

Source DB: PubMed Journal: Protein Sci ISSN： 0961-8368 Impact factor: 6.725

14 in total

Review 1. Efficient Calculation of Enzyme Reaction Free Energy Profiles Using a Hybrid Differential Relaxation Algorithm: Application to Mycobacterial Zinc Hydrolases.

Authors: Juan Manuel Romero; Mariano Martin; Claudia Lilián Ramirez; Victoria Gisel Dumas; Marcelo Adrián Marti
Journal: Adv Protein Chem Struct Biol Date: 2015-07-15 Impact factor: 3.507

2. Inference of macromolecular assemblies from crystalline state.

Authors: Evgeny Krissinel; Kim Henrick
Journal: J Mol Biol Date: 2007-05-13 Impact factor: 5.469

Review 3. CHARMM: the biomolecular simulation program.

Authors: B R Brooks; C L Brooks; A D Mackerell; L Nilsson; R J Petrella; B Roux; Y Won; G Archontis; C Bartels; S Boresch; A Caflisch; L Caves; Q Cui; A R Dinner; M Feig; S Fischer; J Gao; M Hodoscek; W Im; K Kuczera; T Lazaridis; J Ma; V Ovchinnikov; E Paci; R W Pastor; C B Post; J Z Pu; M Schaefer; B Tidor; R M Venable; H L Woodcock; X Wu; W Yang; D M York; M Karplus
Journal: J Comput Chem Date: 2009-07-30 Impact factor: 3.376

Review 4. Fosfomycin versus other antibiotics for the treatment of cystitis: a meta-analysis of randomized controlled trials.

Authors: Matthew E Falagas; Evridiki K Vouloumanou; Antonios G Togias; Maria Karadima; Anastasios M Kapaskelis; Petros I Rafailidis; Stavros Athanasiou
Journal: J Antimicrob Chemother Date: 2010-06-29 Impact factor: 5.790

5. Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.

Authors: Ahmed Gaballa; Gerald L Newton; Haike Antelmann; Derek Parsonage; Heather Upton; Mamta Rawat; Al Claiborne; Robert C Fahey; John D Helmann
Journal: Proc Natl Acad Sci U S A Date: 2010-03-22 Impact factor: 11.205

6. Synthesis of bacillithiol and the catalytic selectivity of FosB-type fosfomycin resistance proteins.

Authors: Alexander P Lamers; Mary E Keithly; Kwangho Kim; Paul D Cook; Donald F Stec; Kelly M Hines; Gary A Sulikowski; Richard N Armstrong
Journal: Org Lett Date: 2012-10-03 Impact factor: 6.005

7. A new crystal form of MshB from Mycobacterium tuberculosis with glycerol and acetate in the active site suggests the catalytic mechanism.

Authors: Simon Gareth Broadley; James Conrad Gumbart; Brandon William Weber; Mohlopheni Jackson Marakalala; Daniel Jacobus Steenkamp; Bryan Trevor Sewell
Journal: Acta Crystallogr D Biol Crystallogr Date: 2012-10-13

X-ray crystallographic structure of BshB, the zinc-dependent deacetylase involved in bacillithiol biosynthesis.

Review 1. Efficient Calculation of Enzyme Reaction Free Energy Profiles Using a Hybrid Differential Relaxation Algorithm: Application to Mycobacterial Zinc Hydrolases.

2. Inference of macromolecular assemblies from crystalline state.

Review 3. CHARMM: the biomolecular simulation program.

Review 4. Fosfomycin versus other antibiotics for the treatment of cystitis: a meta-analysis of randomized controlled trials.

5. Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.

6. Synthesis of bacillithiol and the catalytic selectivity of FosB-type fosfomycin resistance proteins.

7. A new crystal form of MshB from Mycobacterium tuberculosis with glycerol and acetate in the active site suggests the catalytic mechanism.

8. Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus.

9. Histone deacetylase inhibitors through click chemistry.

Review 10. The Role of Bacillithiol in Gram-Positive Firmicutes.

1. X-ray crystallographic structure of BshB, the zinc-dependent deacetylase involved in bacillithiol biosynthesis.