Literature DB >> 3186695

Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.

M R Block1, B S Glick, C A Wilcox, F T Wieland, J E Rothman.   

Abstract

N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport can be rescued by adding back an appropriately prepared cytosol fraction. This complementation assay has allowed us to purify the NEM-sensitive factor, which we term NSF. The NEM-sensitive factor is a tetramer of 76-kDa subunits, and appears to act catalytically, one tetramer leading to the metabolism of numerous transport vesicles.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3186695      PMCID： PMC282295          DOI： 10.1073/pnas.85.21.7852

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

Review 1.  Intracellular aspects of the process of protein synthesis.

Authors:  G Palade
Journal:  Science       Date:  1975-08-01       Impact factor: 47.728

2.  Attachment of terminal N-acetylglucosamine to asparagine-linked oligosaccharides occurs in central cisternae of the Golgi stack.

Authors:  W G Dunphy; R Brands; J E Rothman
Journal:  Cell       Date:  1985-02       Impact factor: 41.582

3.  Sequential intermediates in the pathway of intercompartmental transport in a cell-free system.

Authors:  W E Balch; B S Glick; J E Rothman
Journal:  Cell       Date:  1984-12       Impact factor: 41.582

4.  The glycoprotein that is transported between successive compartments of the Golgi in a cell-free system resides in stacks of cisternae.

Authors:  W A Braell; W E Balch; D C Dobbertin; J E Rothman
Journal:  Cell       Date:  1984-12       Impact factor: 41.582

5.  Transport of vesicular stomatitis virus glycoprotein in a cell-free extract.

Authors:  E Fries; J E Rothman
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

6.  The golgi apparatus: two organelles in tandem.

Authors:  J E Rothman
Journal:  Science       Date:  1981-09-11       Impact factor: 47.728

7.  Characterization of protein transport between successive compartments of the Golgi apparatus: asymmetric properties of donor and acceptor activities in a cell-free system.

Authors:  W E Balch; J E Rothman
Journal:  Arch Biochem Biophys       Date:  1985-07       Impact factor: 4.013

8.  Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.

Authors:  W E Balch; W G Dunphy; W A Braell; J E Rothman
Journal:  Cell       Date:  1984-12       Impact factor: 41.582

9.  Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack.

Authors:  V Malhotra; L Orci; B S Glick; M R Block; J E Rothman
Journal:  Cell       Date:  1988-07-15       Impact factor: 41.582

10.  Density of newly synthesized plasma membrane proteins in intracellular membranes II. Biochemical studies.

Authors:  P Quinn; G Griffiths; G Warren
Journal:  J Cell Biol       Date:  1984-06       Impact factor: 10.539
View more
  175 in total

1.  A mutation in the general membrane trafficking machinery and hydrocephaly.

Authors:  Thomas H Söllner
Journal:  Proc Natl Acad Sci U S A       Date:  2004-02-02       Impact factor: 11.205

Review 2.  Mammalian glycosylation mutants as tools for the analysis and reconstitution of protein transport.

Authors:  A W Brändli
Journal:  Biochem J       Date:  1991-05-15       Impact factor: 3.857

Review 3.  The late stage of autophagy: cellular events and molecular regulation.

Authors:  Jingjing Tong; Xianghua Yan; Li Yu
Journal:  Protein Cell       Date:  2010-11-09       Impact factor: 14.870

4.  Biochemical suppression of small-molecule inhibitors: a strategy to identify inhibitor targets and signaling pathway components.

Authors:  Jeffrey R Peterson; Andres M Lebensohn; Henry E Pelish; Marc W Kirschner
Journal:  Chem Biol       Date:  2006-04

5.  Biochemical analysis of constitutive secretion in a semiintact cell system.

Authors:  S G Miller; H P Moore
Journal:  Cell Biophys       Date:  1991 Oct-Dec

6.  A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast.

Authors:  C G Burd; M Peterson; C R Cowles; S D Emr
Journal:  Mol Biol Cell       Date:  1997-06       Impact factor: 4.138

7.  Single vesicle analysis of endocytic fission on microtubules in vitro.

Authors:  John W Murray; Souvik Sarkar; Allan W Wolkoff
Journal:  Traffic       Date:  2008-02-15       Impact factor: 6.215

8.  GTP gamma S stimulation of endosome fusion suggests a role for a GTP-binding protein in the priming of vesicles before fusion.

Authors:  L S Mayorga; R Diaz; M I Colombo; P D Stahl
Journal:  Cell Regul       Date:  1989-11

9.  Three αSNAP and 10 ATP molecules are used in SNARE complex disassembly by N-ethylmaleimide-sensitive factor (NSF).

Authors:  Niket Shah; Karen N Colbert; Michael D Enos; Daniel Herschlag; William I Weis
Journal:  J Biol Chem       Date:  2014-12-09       Impact factor: 5.157

10.  A pertussis-toxin-sensitive protein controls exocytosis in chromaffin cells at a step distal to the generation of second messengers.

Authors:  J M Sontag; D Thierse; B Rouot; D Aunis; M F Bader
Journal:  Biochem J       Date:  1991-03-01       Impact factor: 3.857
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.