High throughput bar adsorptive microextraction: A simple and effective analytical approach for the determination of nicotine and cotinine in urine samples.

A simple, effective, convenient and environmentally friendly methodology using high throughput bar adsorptive microextraction (HT-BAμE) with microliquid desorption in combination with large volume injection-gas chromatography-mass spectrometry operating in the selected-ion monitoring acquisition mode (LVI-GC-MS(SIM)) was applied for the determination of nicotine and cotinine in urine samples. Under optimized experimental conditions, the developed methodology allowed for linear dynamic ranges between 20.0 and 2000.0 μg L-1 with determination coefficients of 0.9991 and 0.9992, as well as average recovery yields of 61.7-67.5% and 53.9-57.8% for nicotine and cotine, respectively. The developed methodology was applied to monitor urine samples from 86 volunteers having different smoking habits, where nicotine and cotinine were quantified in the range from 23.6 to 2612.6 μg L-1. The target compounds were extracted in a HT-BAμE apparatus, which allows for simultaneous microextraction and subsequent back-extraction of up to 100 samples. This is a major improvement over other microextraction techniques. The data from the proposed methodology were satisfactory and in line with current green analytical chemistry guidelines, and proved to be an effective sample preparation alternative with substantial potential for high throughput bioanalysis.