Enoch Yeung1, Takahiro Inoue1, Hiroshi Matsushita1, Justin Opfermann2, Paige Mass2, Seda Aslan3, Jed Johnson4, Kevin Nelson4, Byeol Kim3, Laura Olivieri2, Axel Krieger3, Narutoshi Hibino5. 1. Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Md. 2. Division of Cardiology, Children's National Medical Center, Washington, DC. 3. Department of Mechanical Engineering, University of Maryland, Baltimore, Md. 4. Nanofiber Solutions, Hilliard, Ohio. 5. Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Md; Department of Cardiac Surgery, University of Chicago/Advocate Children's Hospital, Chicago, Ill. Electronic address: nhibino@surgery.bsd.uchicago.edu.
Abstract
BACKGROUND: The customized vascular graft offers the potential to simplify the surgical procedure, optimize physiological function, and reduce morbidity and mortality. This experiment evaluated the feasibility of a flow dynamic-optimized branched tissue engineered vascular graft (TEVG) customized based on medical imaging and manufactured by 3-dimensional (3D) printing for a porcine model. METHODS: We acquired magnetic resonance angiography and 4-dimensional flow data for the native anatomy of the pigs (n = 2) to design a custom-made branched vascular graft of the pulmonary bifurcation. An optimal shape of the branched vascular graft was designed using a computer-aided design system informed by computational flow dynamics analysis. We manufactured and implanted the graft for pulmonary artery (PA) reconstruction in the porcine model. The graft was explanted at 4 weeks after implantation for further evaluation. RESULTS: The custom-made branched PA graft had a wall shear stress and pressure drop (PD) from the main PA to the branch PA comparable to the native vessel. At the end point, magnetic resonance imaging revealed comparable left/right pulmonary blood flow balance. PD from main PA to branch between before and after the graft implantation was unchanged. Immunohistochemistry showed evidence of endothelization and smooth muscle layer formation without calcification of the graft. CONCLUSIONS: Our animal model demonstrates the feasibility of designing and implanting image-guided, 3D-printed, customized grafts. These grafts can be designed to optimize both anatomic fit and hemodynamic properties. This study demonstrates the tremendous potential structural and physiological advantages of customized TEVGs in cardiac surgery.
BACKGROUND: The customized vascular graft offers the potential to simplify the surgical procedure, optimize physiological function, and reduce morbidity and mortality. This experiment evaluated the feasibility of a flow dynamic-optimized branched tissue engineered vascular graft (TEVG) customized based on medical imaging and manufactured by 3-dimensional (3D) printing for a porcine model. METHODS: We acquired magnetic resonance angiography and 4-dimensional flow data for the native anatomy of the pigs (n = 2) to design a custom-made branched vascular graft of the pulmonary bifurcation. An optimal shape of the branched vascular graft was designed using a computer-aided design system informed by computational flow dynamics analysis. We manufactured and implanted the graft for pulmonary artery (PA) reconstruction in the porcine model. The graft was explanted at 4 weeks after implantation for further evaluation. RESULTS: The custom-made branched PA graft had a wall shear stress and pressure drop (PD) from the main PA to the branch PA comparable to the native vessel. At the end point, magnetic resonance imaging revealed comparable left/right pulmonary blood flow balance. PD from main PA to branch between before and after the graft implantation was unchanged. Immunohistochemistry showed evidence of endothelization and smooth muscle layer formation without calcification of the graft. CONCLUSIONS: Our animal model demonstrates the feasibility of designing and implanting image-guided, 3D-printed, customized grafts. These grafts can be designed to optimize both anatomic fit and hemodynamic properties. This study demonstrates the tremendous potential structural and physiological advantages of customized TEVGs in cardiac surgery.
Authors: C A Caldarone; B W McCrindle; G S Van Arsdell; J G Coles; G Webb; R M Freedom; W G Williams Journal: J Thorac Cardiovasc Surg Date: 2000-12 Impact factor: 5.209
Authors: Joseph A Dearani; Gordon K Danielson; Francisco J Puga; Hartzell V Schaff; Carole W Warnes; David J Driscoll; Cathy D Schleck; Duane M Ilstrup Journal: Ann Thorac Surg Date: 2003-02 Impact factor: 4.330
Authors: E Petrossian; V M Reddy; D B McElhinney; G P Akkersdijk; P Moore; A J Parry; L D Thompson; F L Hanley Journal: J Thorac Cardiovasc Surg Date: 1999-04 Impact factor: 5.209
Authors: Tacy E Downing; Kiona Y Allen; David J Goldberg; Lindsay S Rogers; Chitra Ravishankar; Jack Rychik; Stephanie Fuller; Lisa M Montenegro; James M Steven; Matthew J Gillespie; Jonathan J Rome; Thomas L Spray; Susan C Nicolson; J William Gaynor; Andrew C Glatz Journal: Circ Cardiovasc Interv Date: 2017-09 Impact factor: 6.546
Authors: Cameron Best; Robert Strouse; Kan Hor; Victoria Pepper; Amy Tipton; John Kelly; Toshiharu Shinoka; Christopher Breuer Journal: J Tissue Eng Date: 2018-03-16 Impact factor: 7.813
Authors: Marc Delaney; Vincent Cleveland; Paige Mass; Francesco Capuano; Jason G Mandell; Yue-Hin Loke; Laura Olivieri Journal: Int J Cardiovasc Imaging Date: 2021-11-02 Impact factor: 2.357