Fatma Amer1, Monkez M Yousif2, Heba Mohtady3, Rania A Khattab4, Ergenekon Karagoz5, Khan F M Ayaz6, Noha M Hammad3. 1. Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt; Viral Infection Working Group of International Society of Antimicrobial Chemotherapy VIWG/ISAC; International Society for Infectious Diseases (ISID) council member. 2. Viral Infection Working Group of International Society of Antimicrobial Chemotherapy VIWG/ISAC; Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. 3. Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt; Viral Infection Working Group of International Society of Antimicrobial Chemotherapy VIWG/ISAC. 4. Viral Infection Working Group of International Society of Antimicrobial Chemotherapy VIWG/ISAC; Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt. 5. Viral Infection Working Group of International Society of Antimicrobial Chemotherapy VIWG/ISAC; GATA Haydarpasa Training Hospital, Infectious Diseases and Clinical Microbiology, Istanbul, Turkey. 6. Viral Infection Working Group of International Society of Antimicrobial Chemotherapy VIWG/ISAC; Apollo Hospitals, Dhaka, Bangladesh. Electronic address: kfmayaz@yahoo.com.
Abstract
OBJECTIVES: Egypt ranks first regarding the prevalence of hepatitis C virus (HCV) infection. Many patients have concomitant diseases like kidney disorders requiring hemodialysis, a procedure carrying the hazard of transmitting other hepatitis viruses. The purpose of this study was to investigate for occult hepatitis B virus (HBV), SEN virus (SENV), and torque teno virus (TTV) among chronic HCV patients on maintenance hemodialysis to identify their impacts. METHODS: A total of 325 hemodialysis patients were enrolled and divided into two groups based on HCV RNA testing results. Blood samples were collected before hemodialysis. Sera were tested for hepatitis B core antibodies (anti-HBc) and hepatitis B surface antibodies (anti-HBs) using ELISA. HBV, SENV, and TTV DNA were detected by PCR. The serum alanine aminotransferase (ALT) level was measured. RESULTS: Anti-HBc and HBV DNA were detected in 73.1% and 50.8% of group 1 versus 36.4% and 22.6% of group 2. The serum ALT level was higher in group 1 than group 2. SENV was detected in 11.5% of group 1 versus 8.2% of group 2. TTV was detected in 29% of group 1 versus 27% of group 2. CONCLUSIONS: There is an increased prevalence of occult HBV in our locality among chronic HCV patients undergoing hemodialysis. The existence of SENV and TTV viremia has no clinical impact.
OBJECTIVES: Egypt ranks first regarding the prevalence of hepatitis C virus (HCV) infection. Many patients have concomitant diseases like kidney disorders requiring hemodialysis, a procedure carrying the hazard of transmitting other hepatitis viruses. The purpose of this study was to investigate for occult hepatitis B virus (HBV), SEN virus (SENV), and torque teno virus (TTV) among chronic HCVpatients on maintenance hemodialysis to identify their impacts. METHODS: A total of 325 hemodialysis patients were enrolled and divided into two groups based on HCV RNA testing results. Blood samples were collected before hemodialysis. Sera were tested for hepatitis B core antibodies (anti-HBc) and hepatitis B surface antibodies (anti-HBs) using ELISA. HBV, SENV, and TTV DNA were detected by PCR. The serum alanine aminotransferase (ALT) level was measured. RESULTS: Anti-HBc and HBV DNA were detected in 73.1% and 50.8% of group 1 versus 36.4% and 22.6% of group 2. The serum ALT level was higher in group 1 than group 2. SENV was detected in 11.5% of group 1 versus 8.2% of group 2. TTV was detected in 29% of group 1 versus 27% of group 2. CONCLUSIONS: There is an increased prevalence of occult HBV in our locality among chronic HCVpatients undergoing hemodialysis. The existence of SENV and TTV viremia has no clinical impact.
Authors: Elmoeiz A Elnagi; Thekra N Al-Maqati; Yaser Alnaam; Ahmed A Adam; Ali A Rabaan; Zeinab S Mohamed; Anisah Amer; Hussa L Almarfoi Journal: Saudi J Biol Sci Date: 2021-04-01 Impact factor: 4.219