| Literature DB >> 31863862 |
Xiaofeng Zhou1, Mingshu Wang1, Anchun Cheng2, Qiao Yang1, Ying Wu1, Renyong Jia1, Mafeng Liu1, Dekang Zhu3, Shun Chen1, Shaqiu Zhang1, Xin-Xin Zhao1, Juan Huang1, Sai Mao1, Xumin Ou1, Qun Gao1, Yunya Liu1, Yanling Yu1, Ling Zhang1, Bin Tian4, Leichang Pan4, Mujeeb Ur Rehman4, Xiaoyue Chen1.
Abstract
A colloidal gold strip (CGS) for detecting antibodies to duck plague virus (DPV) was developed. Colloidal gold-labeled DPV gI protein and goat anti-rabbit IgG were dispensed on a conjugate pad as tracers. The recombinant DPV gI protein and rabbit IgG were used as capture reagents at the test line and control line, respectively. The detection limit of this assay was 1:256. Additionally, the CGS did not react with antisera from other common duck diseases, only reacting with anti-DPV serum and yielding a specific and strong red signal. 123 serum samples were tested by CGS and enzyme-linked immunosorbent assay (ELISA), and the results showed good agreement. The CGS test results can be observed in 15 min with the naked eye, should be suitable for clinical testing and large-scale detection.Entities:
Keywords: Colloidal gold strip (CGS); DPV gI protein; Duck plague virus
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Year: 2019 PMID： 31863862 DOI： 10.1016/j.jviromet.2019.113803
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014