Ryosuke Chiba1, Naoto Morikawa1, Koichiro Sera2, Kazuyuki Ishida3, Hiromi Nagashima1, Wataru Shigeeda4, Hiroyuki Deguchi4, Makoto Tomoyasu4, Takako Hosokawa5, Hajime Saito4, Tamotsu Sugai3, Kohei Yamauchi1, Makoto Maemondo1. 1. Department of Internal Medicine, Division of Pulmonary Medicine, Allergy, and Rheumatology, Iwate Medical University School of Medicine Morioka, Iwate, Japan. 2. Cyclotron Research Center, Iwate Medical University School of Medicine Takizawa, Iwate, Japan. 3. Department of Molecular Diagnostic Pathology, Iwate Medical University School of Medicine Morioka, Iwate, Japan. 4. Department of Thoracic Surgery, Iwate Medical University School of Medicine Morioka, Iwate, Japan. 5. Takizawa Laboratory, Japan Radioisotope Association, Takizawa, Iwate, Japan.
Abstract
BACKGROUND: This study aimed to observe the association between trace element concentrations in lung tissue from lung adenocarcinoma cancer (LADC) patients and mutations in the epidermal growth factor receptor (EGFR) and KRAS genes. METHODS: LADC patients who had undergone lung resection were included in this study. Furthermore, twenty patients without lung cancer were included in this study as the control group. Samples were separately collected from both tumor and peritumor tissues. The mutational status was assessed for EGFR mutations, ALK rearrangements and KRAS mutations. Based on these analyses, patients were grouped into three groups: EGFR mutation, KRAS mutation and wild-type groups. The concentrations of various trace elements in the lung tissues were measured by a particle-induced X-ray emission (PIXE) system, and the results were analyzed for statistical significance. RESULTS: A total of 110 LADC patients were included in this study. The median age was 70 years, and 60% of the participants were female. Moreover, 18% and 20% of patients were EGFR- and KRAS-positive, respectively. Thirty-two trace elements were measured, and 18 trace elements were detectable. The concentrations of Fe, Co, Ni, Cu, Zn and Br were significantly higher in the KRAS mutation and wild-type groups than in the control group regardless of whether the samples were from tumor or peritumor tissues. For these 6 trace elements, the concentrations were significantly higher in smokers than in non-smokers. Considering the effect of smoking, differences in the trace element concentrations between each mutational group remained. CONCLUSIONS: Trace elements in the lung may play a role in development of LADC in both smokers and never-smokers. However, prospective studies with larger sample sizes are needed to support this hypothesis. 2019 Translational Lung Cancer Research. All rights reserved.
BACKGROUND: This study aimed to observe the association between trace element concentrations in lung tissue from lung adenocarcinoma cancer (LADC) patients and mutations in the epidermal growth factor receptor (EGFR) and KRAS genes. METHODS: LADC patients who had undergone lung resection were included in this study. Furthermore, twenty patients without lung cancer were included in this study as the control group. Samples were separately collected from both tumor and peritumor tissues. The mutational status was assessed for EGFR mutations, ALK rearrangements and KRAS mutations. Based on these analyses, patients were grouped into three groups: EGFR mutation, KRAS mutation and wild-type groups. The concentrations of various trace elements in the lung tissues were measured by a particle-induced X-ray emission (PIXE) system, and the results were analyzed for statistical significance. RESULTS: A total of 110 LADC patients were included in this study. The median age was 70 years, and 60% of the participants were female. Moreover, 18% and 20% of patients were EGFR- and KRAS-positive, respectively. Thirty-two trace elements were measured, and 18 trace elements were detectable. The concentrations of Fe, Co, Ni, Cu, Zn and Br were significantly higher in the KRAS mutation and wild-type groups than in the control group regardless of whether the samples were from tumor or peritumor tissues. For these 6 trace elements, the concentrations were significantly higher in smokers than in non-smokers. Considering the effect of smoking, differences in the trace element concentrations between each mutational group remained. CONCLUSIONS: Trace elements in the lung may play a role in development of LADC in both smokers and never-smokers. However, prospective studies with larger sample sizes are needed to support this hypothesis. 2019 Translational Lung Cancer Research. All rights reserved.
Entities:
Keywords:
Trace element; adenocarcinoma; driver mutation; lung cancer
Authors: Alberto Ruano-Ravina; María Torres-Durán; Karl T Kelsey; Isaura Parente-Lamelas; Virginia Leiro-Fernández; Ihab Abdulkader; José Abal-Arca; Carmen Montero-Martínez; Iria Vidal-García; Margarita Amenedo; Olalla Castro-Añón; Antonio Golpe-Gómez; Javier González-Barcala; Cristina Martínez; Rosirys Guzmán-Taveras; Mariano Provencio; María José Mejuto-Martí; Alberto Fernández-Villar; Juan Miguel Barros-Dios Journal: Eur Respir J Date: 2016-10-06 Impact factor: 16.671
Authors: Kathryn Hughes Barry; Stella Koutros; Jay H Lubin; Joseph B Coble; Francesco Barone-Adesi; Laura E Beane Freeman; Dale P Sandler; Jane A Hoppin; Xiaomei Ma; Tongzhang Zheng; Michael C R Alavanja Journal: Cancer Causes Control Date: 2012-04-24 Impact factor: 2.506
Authors: María Torres-Durán; Alberto Ruano-Ravina; Isaura Parente-Lamelas; Virginia Leiro-Fernández; José Abal-Arca; Carmen Montero-Martínez; Carolina Pena-Álvarez; Francisco Javier González-Barcala; Olalla Castro-Añón; Antonio Golpe-Gómez; Cristina Martínez; María José Mejuto-Martí; Alberto Fernández-Villar; Juan Miguel Barros-Dios Journal: Eur Respir J Date: 2014-07-17 Impact factor: 16.671
Authors: Xiuyu Cai; Zhenghe Chen; Meiling Deng; Zhiyong Li; Qianchao Wu; Jinwang Wei; Chun Dai; Guan Wang; Chun Luo Journal: Ann Transl Med Date: 2020-09