| Literature DB >> 31848709 |
Viktoria Melcher1, Monika Graf1, Marta Interlandi1,2, Natalia Moreno1, Flavia W de Faria1, Su Na Kim1, Dennis Kastrati1, Sonja Korbanka1, Amelie Alfert1, Joachim Gerß3, Gerd Meyer Zu Hörste4, Wolfgang Hartmann5, Michael C Frühwald6,7, Martin Dugas2, Ulrich Schüller8,9,10, Martin Hasselblatt11, Thomas K Albert1, Kornelius Kerl12.
Abstract
Atypical teratoid/rhabdoid tumors (ATRT) are known for their heterogeneity concerning pathophysiology and outcome. However, predictive factors within distinct subgroups still need to be uncovered. Using multiplex immunofluorescent staining and single-cell RNA sequencing we unraveled distinct compositions of the immunological tumor microenvironment (TME) across ATRT subgroups. CD68+ cells predominantly infiltrate ATRT-SHH and ATRT-MYC and are a negative prognostic factor for patients' survival. Within the murine ATRT-MYC and ATRT-SHH TME, Cd68+ macrophages are core to intercellular communication with tumor cells. In ATRT-MYC distinct tumor cell phenotypes express macrophage marker genes. These cells are involved in the acquisition of chemotherapy resistance in our relapse xenograft mouse model. In conclusion, the tumor cell-macrophage interaction contributes to ATRT-MYC heterogeneity and potentially to tumor recurrence.Entities:
Keywords: Atypical teratoid/rhabdoid tumor; Cell interaction; Chemotherapy resistance; Single-cell RNA sequencing; Tumor microenvironment; Tumor-associated macrophages
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Year: 2019 PMID： 31848709 DOI： 10.1007/s00401-019-02116-7
Source DB: PubMed Journal: Acta Neuropathol ISSN： 0001-6322 Impact factor: 17.088