Charles Cassius1,2,3,4, Mylene Branchtein5, Maxime Battistella1,6, Reyhan Amode1,2,4, Clémence Lepelletier1,2,4, Marie Jachiet2,4, Adèle de Masson1,2, Laure Frumholtz2,4, François Chasset4,7,8, Zahir Amoura9, Alexis Mathian9, Assia Samri9, Jean-Benoit Monfort7, Claude Bachmeyer10, Djaouida Bengoufa11, Florence Cordoliani2, Martine Bagot1,2, Armand Bensussan1, Jean-David Bouaziz1,2,4, Hélène Le Buanec1. 1. Université de Paris, Inserm U976 - HIPI Unit, Institut de Recherche Saint-Louis. 2. Dermatology Department, AP-HP, Hôpital Saint-Louis, Paris. 3. Université Catholique de Louvain, CHU UCL Namur, Godinne. 4. EMSED (etude des maladies systémiques en Dermatologie), Paris, France. 5. Institut Jules Bordet, Université Libre de Belgique, Bruxelles, Belgium. 6. Pathology Department, AP-HP, Hôpital Saint-Louis. 7. Dermatology Department, AP-HP, Hôpital Tenon. 8. Sorbonne Université, Faculté de médecine sorbonne université, Paris. 9. Groupement Hospitalier Pitié-Salpétrère, AP-HP, French National Referral Center for Systemic Lupus Erythematosus, Antiphospholipid Antibody Syndrome and Other Autoimmune Disorders, Service de Mé decine Interne 2, Institut E3M, Inserm UMRS, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris). 10. Internal Medicine Department, AP-HP, Hôpital Tenon. 11. Immunobiology Department, AP-HP, Hôpital Saint-Louis, Paris, France.
Abstract
OBJECTIVES: Mucosal-associated invariant T (MAIT) cells are innate-like lymphocytes that are important for antibacterial immunity and may have regulatory roles. MAIT cells are decreased during SLE. However, their frequencies and phenotype have not been investigated in DM. We studied MAIT cell frequencies and phenotype in DM patients with active and inactive disease (after treatment). METHODS: Peripheral blood flow cytometry analysis of MAIT cells was compared between DM (n = 22), SLE (n = 10), psoriasis (n = 7) and atopic dermatitis (n = 5) patients, and healthy controls (n = 19). RESULTS: A dramatic decrease of circulating MAIT cell frequency was observed in active DM and SLE patients compared with healthy controls and other inflammatory skin diseases [active DM: median = 0.25% (interquartile range 0.19-0.6%), P < 0.0001; active SLE: median = 0.61 (0.55-0.77), P < 0.0001 vs healthy controls: 2.32% (1.18-4.45%)]. MAIT cells from active DM patients had an abnormal phenotype including increased expression of CD25 and cytotoxic T-lymphocyte-associated protein 4 that correlated with their low frequency in the blood. CONCLUSION: In DM, peripheral blood MAIT cells are dramatically reduced and have an activated/exhausted phenotype that may be linked to increased activation-induced cell death.
OBJECTIVES: Mucosal-associated invariant T (MAIT) cells are innate-like lymphocytes that are important for antibacterial immunity and may have regulatory roles. MAIT cells are decreased during SLE. However, their frequencies and phenotype have not been investigated in DM. We studied MAIT cell frequencies and phenotype in DM patients with active and inactive disease (after treatment). METHODS: Peripheral blood flow cytometry analysis of MAIT cells was compared between DM (n = 22), SLE (n = 10), psoriasis (n = 7) and atopic dermatitis (n = 5) patients, and healthy controls (n = 19). RESULTS: A dramatic decrease of circulating MAIT cell frequency was observed in active DM and SLEpatients compared with healthy controls and other inflammatory skin diseases [active DM: median = 0.25% (interquartile range 0.19-0.6%), P < 0.0001; active SLE: median = 0.61 (0.55-0.77), P < 0.0001 vs healthy controls: 2.32% (1.18-4.45%)]. MAIT cells from active DM patients had an abnormal phenotype including increased expression of CD25 and cytotoxic T-lymphocyte-associated protein 4 that correlated with their low frequency in the blood. CONCLUSION: In DM, peripheral blood MAIT cells are dramatically reduced and have an activated/exhausted phenotype that may be linked to increased activation-induced cell death.
Authors: Vera Plužarić; Mario Štefanić; Martina Mihalj; Maja Tolušić Levak; Ivanka Muršić; Ljubica Glavaš-Obrovac; Martin Petrek; Peter Balogh; Stana Tokić Journal: Front Immunol Date: 2020-12-03 Impact factor: 7.561