| Literature DB >> 31844523 |
Maryam Salari1, Dimitrios Bitounis1, Kunal Bhattacharya1, Georgios Pyrgiotakis1, Zhenyuan Zhang1, Emilia Purington2, William Gramlich3, Yohann Grondin4, Rick Rogers4, Douglas Bousfield2, Philip Demokritou1.
Abstract
The rapid adoption of nanocellulose-based engineered nanomaterials (CNM) by many industries generates environmental health and safety (EHS) concerns. This work presents the development of fluorescently tagged CNM which can be used to study their interactions with biological systems. Specifically, cellulose nano-fibrils and cellulose nano-crystals with covalently attached fluorescein isothiocyanate (FITC) molecules on their surface were synthesized. The fluorescence of the FITC-tagged materials was assessed along with potential FITC detachment under pH conditions encountered in the human gastrointestinal tract, in intracellular compartments, and in cell culture media. Finally, the potential cytotoxicity due to the presence of FITC molecules on the surface of CNM was assessed using a cellular gut epithelium model. The results showed that neither FITC-CNF nor FITC-CNC were cytotoxic and that they have a comparable bioactivity to their untagged counterparts, rendering them suitable for biological studies.Entities:
Year: 2019 PMID： 31844523 PMCID： PMC6914317 DOI： 10.1039/C8EN01381K
Source DB: PubMed Journal: Environ Sci Nano