Fernanda Loayza-Villa1, Liseth Salinas2, Nathalie Tijet3, Fernando Villavicencio4, Rafael Tamayo4, Stephanie Salas4, Ruth Rivera4, Jose Villacis5, Carolina Satan4, Liliana Ushiña4, Olga Muñoz4, Jeannette Zurita6, Roberto Melano7, Jorge Reyes8, Gabriel A Trueba2. 1. Universidad San Franscisco de Quito, Av. Diego de Robles y Pampite, Quito, Ecuador. Electronic address: mfloayzav@usfq.edu.ec. 2. Universidad San Franscisco de Quito, Av. Diego de Robles y Pampite, Quito, Ecuador. 3. Public Health Ontario, Toronto, ON, Canada. 4. Instituto Nacional de Investigación en Salud Pública 'Dr. Leopoldo Izquieta Perez', Quito, Ecuador. 5. Instituto Nacional de Investigación en Salud Pública 'Dr. Leopoldo Izquieta Perez', Quito, Ecuador; Facultad de Medicina, Pontificia Universidad Católica del Ecuador, Quito, Ecuador. 6. Unidad de Investigaciones en Biomedicina, Zurita & Zurita Laboratorios, Quito, Ecuador. 7. Public Health Ontario, Toronto, ON, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada. 8. Universidad Central del Ecuador, Facultad de Ciencias Químicas, Ciudadela Universitaria - Avenida América, Quito, Ecuador.
Abstract
OBJECTIVE: The aim of this study was to detect potential animal reservoirs of Escherichia coli carrying the mcr-1 gene in an Ecuadorian household. METHODS: The mobile colistin-resistance gene, mcr-1, was first detected in Ecuador in a commensal E. coli isolate from a boy. A cross-sectional study was performed to detect the possible source of colistin-resistant E. coli in the boy's household. Faecal swabs and soil faecal samples were collected from companion animals. Samples were plated on selective media to isolate colistin-resistant E. coli and isolates were submitted to PCR detection of mcr-1, pulsed field gel electrophoresis (PFGE), and multi-locus sequences typing (MLST). Moreover, the genomes of all the isolates were sequenced. RESULTS: Three different colistin-resistant E. coli sequence types (ST3941, 1630 and 2170), corresponding to three PFGE patterns, were obtained from a chicken and two dogs; these isolates were different from the human isolate (ST609). By whole-genome sequencing, the mcr-1.1 gene was found on IncI2 plasmids with very high nucleotide identity. CONCLUSIONS: Our results indicate a polyclonal dissemination of mcr-1.1 in the environment surrounding the first MCR-producing E. coli strain reported in Ecuador. Our findings support the idea of lateral dissemination of mcr-1.1 gene between unrelated E. coli isolates. Crown
OBJECTIVE: The aim of this study was to detect potential animal reservoirs of Escherichia coli carrying the mcr-1 gene in an Ecuadorian household. METHODS: The mobile colistin-resistance gene, mcr-1, was first detected in Ecuador in a commensal E. coli isolate from a boy. A cross-sectional study was performed to detect the possible source of colistin-resistant E. coli in the boy's household. Faecal swabs and soil faecal samples were collected from companion animals. Samples were plated on selective media to isolate colistin-resistant E. coli and isolates were submitted to PCR detection of mcr-1, pulsed field gel electrophoresis (PFGE), and multi-locus sequences typing (MLST). Moreover, the genomes of all the isolates were sequenced. RESULTS: Three different colistin-resistant E. coli sequence types (ST3941, 1630 and 2170), corresponding to three PFGE patterns, were obtained from a chicken and two dogs; these isolates were different from the human isolate (ST609). By whole-genome sequencing, the mcr-1.1 gene was found on IncI2 plasmids with very high nucleotide identity. CONCLUSIONS: Our results indicate a polyclonal dissemination of mcr-1.1 in the environment surrounding the first MCR-producing E. coli strain reported in Ecuador. Our findings support the idea of lateral dissemination of mcr-1.1 gene between unrelated E. coli isolates. Crown
Authors: Carlos Bastidas-Caldes; Juan Ochoa; Laura Guerrero-Latorre; Carlos Moyota-Tello; Wilson Tapia; Joaquín María Rey-Pérez; Maria Isabel Baroja Journal: Int J Environ Res Public Health Date: 2022-04-14 Impact factor: 4.614
Authors: Raquel Girardello; Carlos Morais Piroupo; Joaquim Martins; Marcia Helena Maffucci; Ana Paula Cury; Maria Renata Gomes Franco; Fernanda de Mello Malta; Natália Conceição Rocha; João Renato Rebello Pinho; Flavia Rossi; Alberto José da Silva Duarte; João Carlos Setubal Journal: Front Microbiol Date: 2021-06-09 Impact factor: 5.640