Jing-Zhi Wan1, Rui Wang1, Zhi-Yong Zhou1, Li-Li Deng1, Chang-Cheng Zhang1, Chao-Qi Liu1, Hai-Xia Zhao1, Dongfan Wang1, Cheng-Fu Yuan1, Yu-Min He1, Yao-Yan Dun1, Ding Yuan1, Ting Wang1.
Abstract
BACKGROUND: Oxidative stress and mitochondrial dysfunction play a vital role in the pathogenesis of brain aging. Saponins from Panax japonicus (SPJ) have attracted much attention for its potential to attenuate age-related oxidative stress as the main ingredient in rhizomes of Panax japonicus.
OBJECTIVE: This study aimed to investigate the neuroprotective effects of SPJ on natural aging rats as well as the underlying mechanisms regarding oxidative stress and mitochondrial pathway.
METHODS: Sprague-Dawley rats were divided into control groups (3-, 9-, 15- and 24-month old groups) and SPJ-treated groups. For SPJ-treated groups, SPJ were orally administrated to 18-month old rats at the doses of 10 mg/kg, 30 mg/kg and 60 mg/kg once daily. Control groups were given the same volume of saline. After treatment of SPJ or saline for six months, the cortex and hippocampus were rapidly harvested and deposited at -80°C after the rats were decapitated under anesthesia. The neuroprotective effects of SPJ were estimated by histopathological observation, TUNEL detection, biochemical determination and western blotting.
RESULTS: SPJ improved pathomorphological changes in neuronal cells and decreased apoptosis in the cortex and hippocampus of aging rats, increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), Na+/K+-ATPase, Ca2+-ATPase and Ca2+/Mg2+-ATPase whereas decreased malondialdehyde (MDA) contents in the cortex of aging rats. Furthermore, SPJ increased silent mating type information regulation 2 homolog-1 (SIRT1) protein expression, decreased acetylated level of peroxisomeproliferator-activated receptor-γ coactivator-1α (PGC-1α) in the cortex and hippocampus of aging rats, reversed the aging-induced decline of Forkhead box O3 (Foxo3a), superoxide dismutase 2 (SOD2), microtubule-associated protein light chain 3 (LC3II) and Beclin1 levels in the cortex and hippocampus.
CONCLUSION: Our data showed that SPJ conferred neuroprotection partly through regulation of oxidative stress and mitochondria related pathways in aging rats. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
BACKGROUND: Oxidative stress and mitochondrial dysfunction play a vital role in the pathogenesis of brain aging. Saponins from Panax japonicus (SPJ) have attracted much attention for its potential to attenuate age-related oxidative stress as the main ingredient in rhizomes of Panax japonicus.
OBJECTIVE: This study aimed to investigate the neuroprotective effects of SPJ on natural aging rats as well as the underlying mechanisms regarding oxidative stress and mitochondrial pathway.
METHODS: Sprague-Dawley rats were divided into control groups (3-, 9-, 15- and 24-month old groups) and SPJ-treated groups. For SPJ-treated groups, SPJ were orally administrated to 18-month old rats at the doses of 10 mg/kg, 30 mg/kg and 60 mg/kg once daily. Control groups were given the same volume of saline. After treatment of SPJ or saline for six months, the cortex and hippocampus were rapidly harvested and deposited at -80°C after the rats were decapitated under anesthesia. The neuroprotective effects of SPJ were estimated by histopathological observation, TUNEL detection, biochemical determination and western blotting.
RESULTS: SPJ improved pathomorphological changes in neuronal cells and decreased apoptosis in the cortex and hippocampus of aging rats, increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), Na+/K+-ATPase, Ca2+-ATPase and Ca2+/Mg2+-ATPase whereas decreased malondialdehyde (MDA) contents in the cortex of aging rats. Furthermore, SPJ increased silent mating type information regulation 2 homolog-1 (SIRT1) protein expression, decreased acetylated level of peroxisomeproliferator-activated receptor-γ coactivator-1α (PGC-1α) in the cortex and hippocampus of aging rats, reversed the aging-induced decline of Forkhead box O3 (Foxo3a), superoxide dismutase 2 (SOD2), microtubule-associated protein light chain 3 (LC3II) and Beclin1 levels in the cortex and hippocampus.
CONCLUSION: Our data showed that SPJ conferred neuroprotection partly through regulation of oxidative stress and mitochondria related pathways in aging rats. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
Entities:
Keywords:
Aging; Autophagy; Mitochondria; Oxidative stress; Saponins from Panax japonicus
Year: 2019
PMID： 31840608 DOI： 10.2174/1389201021666191216114815
Source DB: PubMed Journal: Curr Pharm Biotechnol ISSN： 1389-2010 Impact factor: 2.837