Impact of spent mushroom substrate on Cd immobilization and soil property.

This study aims to evaluate the potential of fresh spent mushroom substrate (SMS) in Cd immobilization and soil improvement, compared with spent mushroom substrate biochar (SMSB) and spent mushroom substrate compost (SMSC). A simulating remediation experiment was conducted with soil at Cd concentration of 0.6, 1.2, 1.8, and 2.4 mg kg-1 and amendment addition ratio of 0.5%, 1%, 2%, and 4% for 90 days. At the end of incubation, it was found that 4%SMS addition showed the best effect both on Cd immobilization and soil improvement. It decreased Cd exchangeable fraction ratio by 52.77% (16.30% higher than 4%SMSC) and increased residual fraction ratio by 65.28% (36.34% and 49.64% higher than 4%SMSB and 4%SMSC, respectively); increased soil pH, EC, and CEC by 10.43% (3.83% higher than 4%SMSC), 11.54%, and 29.72%; and increased urease activity, sucrase activity, and catalase activity by 125.61% (43.90% and 8.54% higher than 4%SMSB and 4%SMSC, respectively), 79.46% (35.35% and 14.02% higher than 4%SMSB and 4%SMSC, respectively), and 75.68% (29.73% higher than 4%SMSB), compared with control treatment (CK) respectively. The results demonstrate that 4%SMS can be used as amendments for cadmium-contaminated soils.