Tania Mara Rodrigues Simões1,2, Rosangela da Silva2, Bianca Bianco3, Fernando Luiz Affonso Fonseca4, Dirceu Solé1, Roseli Oselka Saccardo Sarni5,6. 1. Discipline of Allergy, Clinical Immunology and Rheumatology, Department of Pediatrics, Federal University of São Paulo, São Paulo, Brazil. 2. Nutrition Faculty, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil. 3. Discipline of Genetics and Ideia Fertil Institute, Human Reproduction and Genetics Center, Faculdade de Medicina do ABC, Santo André, Brazil. 4. Laboratory of Clinical Analyses, Faculdade de Medicina do ABC, Santo André, Brazil. 5. Discipline of Allergy, Clinical Immunology and Rheumatology, Department of Pediatrics, Federal University of São Paulo, São Paulo, Brazil, rssarni@gmail.com. 6. Discipline of Pediatric Clinic, Faculdade de Medicina do ABC, Santo André, Brazil, rssarni@gmail.com.
Abstract
BACKGROUND: In children, nutritional status of vitamin D (vitD), frequency of vitD receptor (VDR) gene polymorphisms and their associations with overweight and asthma remain controversial. OBJECTIVES: To evaluate the nutritional status of vitD and the frequency of VDR gene polymorphisms, as well as identify their associations with nutritional status and asthma. METHODS: A cross-sectional study was conducted with schoolchildren (n = 262; mean age = 8.7 ± 1.3 years). Clinical history, anthropometric measurements, and serum 25-hydroxy vitD were evaluated. Four VDR gene polymorphisms were identified and genotypes, alleles, and haplotypes were calculated. RESULTS: The serum vitD levels were found at 85.1%, within normal range. The FokI AA genotype was more frequent in asthmatics compared to healthy controls (10 vs. 1%, p < 0.05), while the GG genotype was less frequent (45.0 vs. 55.2%, p < 0.05). The frequency of the TT allele for the ApaI was higher among asthmatic eutrophic children (60.9 vs. 29.4%, p < 0.05) and that of the TT allele for the BsmI was higher among asthmatic overweight children (35.3 vs. 4.4%, p < 0.05). CONCLUSIONS: Insufficiency in vitD was low. Two wild-type alleles (AA) of FokI were identified as risk factor for the development of asthma, while GG alleles appears to be a protective factor. To have polymorphic alleles (TT) of ApaI seems to be a risk factor for asthma in children with normal weight, while that of BsmI seems to be a risk factor for asthma in overweight condition. Serum vitD was not different among analyzed genotypes.
BACKGROUND: In children, nutritional status of vitamin D (vitD), frequency of vitD receptor (VDR) gene polymorphisms and their associations with overweight and asthma remain controversial. OBJECTIVES: To evaluate the nutritional status of vitD and the frequency of VDR gene polymorphisms, as well as identify their associations with nutritional status and asthma. METHODS: A cross-sectional study was conducted with schoolchildren (n = 262; mean age = 8.7 ± 1.3 years). Clinical history, anthropometric measurements, and serum 25-hydroxyvitD were evaluated. Four VDR gene polymorphisms were identified and genotypes, alleles, and haplotypes were calculated. RESULTS: The serum vitD levels were found at 85.1%, within normal range. The FokI AA genotype was more frequent in asthmatics compared to healthy controls (10 vs. 1%, p < 0.05), while the GG genotype was less frequent (45.0 vs. 55.2%, p < 0.05). The frequency of the TT allele for the ApaI was higher among asthmatic eutrophic children (60.9 vs. 29.4%, p < 0.05) and that of the TT allele for the BsmI was higher among asthmatic overweight children (35.3 vs. 4.4%, p < 0.05). CONCLUSIONS: Insufficiency in vitD was low. Two wild-type alleles (AA) of FokI were identified as risk factor for the development of asthma, while GG alleles appears to be a protective factor. To have polymorphic alleles (TT) of ApaI seems to be a risk factor for asthma in children with normal weight, while that of BsmI seems to be a risk factor for asthma in overweight condition. Serum vitD was not different among analyzed genotypes.
Authors: Alana Alcântara Galvão; Flávia de Araújo Sena; Emília Maria Medeiros de Andrade Belitardo; Maria Borges Rabelo de Santana; Gustavo Nunes de Oliveira Costa; Álvaro Augusto Cruz; Maurício Lima Barreto; Ryan Dos Santos Costa; Neuza Maria Alcantara-Neves; Camila Alexandrina Figueiredo Journal: Allergy Asthma Clin Immunol Date: 2020-07-09 Impact factor: 3.406
Authors: Laura Tamasauskiene; Ieva Golubickaite; Rasa Ugenskiene; Nikolajs Sjakste; Natalia Paramonova; Lawrence Shih-Hsin Wu; Lawrence Shih-Jiu-Yao Wang; Brigita Sitkauskiene Journal: Immun Inflamm Dis Date: 2021-08-03