Liang Sheng1, Rong Wei2. 1. Department of Dermatology, Guizhou Provincial People's Hospital, Guiyang, China, shengliang951@163.com. 2. Department of Dermatology, The Second People's Hospital of Guiyang, Guiyang, China.
Abstract
BACKGROUND: Melanoma is one of the most aggressive and high mortality skin cancers in the world. Long non-coding RNA-CASC15, as a carcinogen, plays an important role in a variety of tumorigenesis; however, the role and underlying mechanism of CASC15 in melanoma remain unclear. METHODS: Quantitative real-time polymerase chain reaction was applied to explore CASC15 and β-catenin expression in melanoma tissues and cells. Western blotting was carried out to investigate β-catenin, glycogen synthase kinase-3β, Survivin, Bax, Bcl-2, and epithelial-mesenchymal transition (EMT)-related protein expression level. Cell proliferation, apoptosis, migration, and invasion were observed by colony formation assay, flow cytometry, and transwell migration and invasion assays, respectively. The activity of Wnt/β-catenin signaling pathway was measured by Topflash luciferase reporter assay. RESULTS: The expression of CASC15 and β-catenin was upregulated in melanoma tissues and cells. Knockdown of CASC15 suppressed Wnt/β-catenin signaling pathway and inhibited β-catenin expression. Furthermore, inhibition of CASC15 decreased proliferation and increased apoptosis of melanoma cells by downregulating Survivin and Bcl-2 and upregulating Bax in A375 and SK-MEL-28 cells. Silencing of CASC15 inhibited migration and invasion of melanoma cells by repressing EMT process. CONCLUSION: Our study demonstrated that CASC15 promoted the proliferation, migration, and invasion of melanoma cells via activating Wnt/β-catenin signaling pathway, implying that CASC15 might be a potential therapeutic target and prognostic biomarker for melanoma.
BACKGROUND:Melanoma is one of the most aggressive and high mortality skin cancers in the world. Long non-coding RNA-CASC15, as a carcinogen, plays an important role in a variety of tumorigenesis; however, the role and underlying mechanism of CASC15 in melanoma remain unclear. METHODS: Quantitative real-time polymerase chain reaction was applied to explore CASC15 and β-catenin expression in melanoma tissues and cells. Western blotting was carried out to investigate β-catenin, glycogen synthase kinase-3β, Survivin, Bax, Bcl-2, and epithelial-mesenchymal transition (EMT)-related protein expression level. Cell proliferation, apoptosis, migration, and invasion were observed by colony formation assay, flow cytometry, and transwell migration and invasion assays, respectively. The activity of Wnt/β-catenin signaling pathway was measured by Topflash luciferase reporter assay. RESULTS: The expression of CASC15 and β-catenin was upregulated in melanoma tissues and cells. Knockdown of CASC15 suppressed Wnt/β-catenin signaling pathway and inhibited β-catenin expression. Furthermore, inhibition of CASC15 decreased proliferation and increased apoptosis of melanoma cells by downregulating Survivin and Bcl-2 and upregulating Bax in A375 and SK-MEL-28 cells. Silencing of CASC15 inhibited migration and invasion of melanoma cells by repressing EMT process. CONCLUSION: Our study demonstrated that CASC15 promoted the proliferation, migration, and invasion of melanoma cells via activating Wnt/β-catenin signaling pathway, implying that CASC15 might be a potential therapeutic target and prognostic biomarker for melanoma.
Authors: Jianyong Sun; Yanlu Xiong; Kuo Jiang; Bo Xin; Tongtong Jiang; Renji Wei; Yuankang Zou; Hong Tan; Tao Jiang; Angang Yang; Lintao Jia; Lei Wang Journal: J Exp Clin Cancer Res Date: 2021-01-06
Authors: Margo Tuerlings; Marcella van Hoolwerff; Jessica M van Bokkum; H Eka D Suchiman; Nico Lakenberg; Demiën Broekhuis; Rob G H H Nelissen; Yolande F M Ramos; Hailiang Mei; Davy Cats; Rodrigo Coutinho de Almeida; Ingrid Meulenbelt Journal: Rheumatology (Oxford) Date: 2022-07-06 Impact factor: 7.046