Karine Marion-Sanchez1, Claude Olive2, Marie-Georges Platon2, Myriam Cesarine2, Christian Derancourt3, Karine Pailla2. 1. CHU Martinique, Department of Hospital Hygiene, F-97200 Fort-de-France, Martinique. 2. CHU Martinique, Bacteriology Laboratory, F-97200 Fort-de-France, Martinique. 3. EA 7524, Université des Antilles, F-97200 Fort-de-France, Martinique; CH Briançon F-05100 Briançon, France; CH Gap, Department of Internal Medicine, F-05007 Gap, France.
Abstract
BACKGROUND: Hospital reservoirs of Achromobacter xylosoxidans, responsible for nosocomial infections, are poorly known. METHODS: We examined the growth, survival and biofilm formation of five A. xylosoxidans strains for up to 2 y in distilled, dialysis or microfiltered water. Each strain was inoculated at 102 CFU/ml without adding nutrients. RESULTS: All strains grew at a level of 3x103 to 1.5x107 CFU/ml; each strain showed a preferred water type. Strains isolated from quaternary ammoniums showed the highest ability to grow and form biofilms in nutrient-poor waters. CONCLUSION: Medical waters and notably sterile distilled water bottles appear to be long-lasting reservoirs of A. xylosoxidans.
BACKGROUND: Hospital reservoirs of Achromobacter xylosoxidans, responsible for nosocomial infections, are poorly known. METHODS: We examined the growth, survival and biofilm formation of five A. xylosoxidans strains for up to 2 y in distilled, dialysis or microfiltered water. Each strain was inoculated at 102 CFU/ml without adding nutrients. RESULTS: All strains grew at a level of 3x103 to 1.5x107 CFU/ml; each strain showed a preferred water type. Strains isolated from quaternary ammoniums showed the highest ability to grow and form biofilms in nutrient-poor waters. CONCLUSION: Medical waters and notably sterile distilled water bottles appear to be long-lasting reservoirs of A. xylosoxidans.
Authors: Adam M Pickrum; Orlando DeLeon; Aaron Dirck; Maxx H Tessmer; Molly O Riegert; Julie A Biller; Nathan A Ledeboer; John R Kirby; Dara W Frank Journal: Infect Immun Date: 2020-06-22 Impact factor: 3.441