Fluorescence quenching effects of carbon nano-structures (Graphene Oxide and Nano Diamond) coupled with Methylene Blue.

Here, the effect of Graphene Oxide (GO) and Nano Diamond (ND) carriers on the spectral properties of the fluorescence emissions of MB suspensions in the form of (MB + GO) and (MB + ND) biomaterials are investigated. The strong affinity of MB fluorophores with GO/ND nanostructures lead to the chemical bonding formation that affects the quenching coefficient and spectral shift. According to Stern-Volmer linearity despite, the excited (MB + GO) is strongly quenched due to its privileged bonding affinity, however the (MB + ND) does not. Furthermore, the corresponding quenching coefficients are measured. In fact, GO additives in the MB suspension gives rise to a sensible blue shift due to its surface functionality while no spectral shift takes place in the case of (MB + ND). We have shown that the complex formation such as (MB + GO) is strongly correlated to the GO quenching coefficient due to the hydrogen bonding and π - π staking, whereas there is a loose dependence with the blue shift phenomena. Furthermore, we have compared the quenching coefficients of Rd6G and DOX with MB fluorophore to attest the quenching coefficient is strongly correlated to the molecular structure and its active sites. The findings could be helpful in the course of simultaneous PDT and fluorescence imaging.