| Literature DB >> 31826432 |
Jinyan Gu1, Haihui Zhang2, Jixian Zhang1, Chaoting Wen1, Haile Ma3, Yuqing Duan4, Yuanqing He5.
Abstract
To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol. The results implied that except for neutral sugar, four contained proteins and uronic acids in their structure, which were further confirmed by the ultraviolet and infrared spectra. Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios. In addition, SPC-70 exhibited stronger antioxidant activity in vitro than the other three components. SPCs could significantly promote macrophage proliferation, NO release and phagocytosis. Thus, these results provided a reference for applications of S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture.Entities:
Keywords: Antioxidant activity; Characterization; Fractional precipitation; Immunoactivity; Sagittaria sagittifolia L. polysaccharide; Ultrasonic assisted extraction
Mesh：
Substances：
Year: 2019 PMID： 31826432 DOI： 10.1016/j.carbpol.2019.115355
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381