| Literature DB >> 31820285 |
Limiao Chen1,2, Yisheng Fang1,2, Xiangyong Li3, Kai Zeng4, Haifeng Chen1,2, Hengbin Zhang4, Hongli Yang1,2, Dong Cao1,2, Qingnan Hao1,2, Songli Yuan1,2, Chanjuan Zhang1,2, Wei Guo1,2, Shuilian Chen1,2, Zhonglu Yang1,2, Zhihui Shan1,2, Xiaojuan Zhang1,2, Dezhen Qiu1,2, Yong Zhan5, Xin-An Zhou6,7.
Abstract
KEY MESSAGE: Drought tolerance level of 136 soybean genotypes, the correlations among traits were evaluated, and several important drought-tolerant genotypes, traits, SNPs and genes were possibly useful for soybean genetic breeding. Drought is an adverse environmental factor affecting crops growth, development, and yield. Promising genotypes and genes with improved tolerance to drought are probably effective ways to alleviate the situation. In this study, our main task was to determine drought tolerance level of 136 soybean genotypes, the correlations among physiological and agronomic traits under drought, and drought-tolerant single nucleotide polymorphism (SNPs) and genes. In this study, twenty-six varieties were identified as excellent tolerant genotypes to stress among which S14, S93 and S135 with high drought-tolerant index (DTI > 1.3) and yield (Y > 300 kg). Fourteen varieties were identified as drought-sensitive genotypes, such as S25, S45 and S58, with low drought-tolerant index (DTI < 0.5). 422 SNPs and 302 genes correlated with seed number per plant (SNPP), maturity (M), number of seeds per pod (NSPP), node number of main stem (NNMS), Stem diameter (SD) and pull stem (PS) were detected under well-watered and drought conditions by genome-wide association study (GWAS). Among them, we found SNPs (Chr 3:1758920-1958934) between drought-tolerant and sensitive genotypes. 13 genes (Glyma.03G017800, Glyma.03G018000, Glyma.03G018200, Glyma.03G018400, Glyma.03G018500, Glyma.03G018600, Glyma.03G018700, Glyma.03G018800, Glyma.03G018900, Glyma.03G019000, Glyma.03G019100, Glyma.03G019200, Glyma.03G019300) correlated with NNMS were detected. By qRT-PCR, the expression level of Glyma.03G018000 and Glyma.03G018900 in drought-tolerant varieties was significantly increased, but low or no expression in sensitive varieties under drought stress. This study provides important drought-tolerant genotypes, traits, SNPs and potential genes, possibly useful for soybean genetic breeding.Entities:
Keywords: Candidate genes; Drought stress; Drought-tolerant index; Physiological and agronomic traits; SNPs; Soybean
Mesh：
Year: 2019 PMID： 31820285 DOI： 10.1007/s11103-019-00934-7
Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076