Calcium Pyrophosphate And Monosodium Urate Activate The NLRP3 Inflammasome Within Bladder Urothelium Via Reactive Oxygen Species And TXNIP.

OBJECTIVE: To investigate the in vitro activation of the NLRP3 inflammasome within bladder urothelium by stone-forming components. Further, to describe the contributions of reactive oxygen species (ROS) and thioredoxin-interacting protein (TXNIP), an important structural component of the inflammasome, to this activation.
METHODS: Urothelial cells were harvested and incubated overnight. For agonist studies, cells were treated with varying concentrations of calcium pyrophosphate (CPPD) and monosodium urate (MSU). For inhibitor studies, cells were treated with either N-acetylcysteine (NAC) (1 hr) or Verapamil (4 hrs) prior to incubation with either CPPD (62.5 ug/mL) or MSU (1.25 ug/mL) for 24 hrs. Untreated controls were incubated with ATP (1.25 mM) for 1 hr to maximally stimulate NLRP3 inflammasome activity (measured as caspase-1 cleavage of the fluorogenic substrate Ac-YVAD-AFC). Results are reported as a percentage of maximum ATP response.
RESULTS: CPPD and MSU activate caspase-1 in urothelial cells in a dose-dependent manner, reaching ~50% and ~25% of the ATP response, respectively. Pre-treatment with the general ROS scavenger NAC reduces this activation in a dose-dependent manner. Additionally, activation was suppressed through treatment with Verapamil, a known downregulator of TXNIP expression.
CONCLUSION: The stone components CPPD and MSU activate NLRP3 in an ROS and TXNIP-dependent manner in bladder urothelium. These findings demonstrate the importance of ROS and TXNIP, and suggest that targeting either may be a way to decrease stone-dependent NLRP3 inflammation within the bladder.

Introduction

Urine contains many noxious chemical moieties, such as organic acids and salts, which can irritate or inflame the lumen of the urinary tract. In patients who are particularly sensitive to these, including those who have defects in the protective glycosaminoglycan (GAG) layer, they can cause urinary symptoms of urgency and frequency or even pain. The most potent forms of these irritants are the components that can crystallize into urinary stones. Once they have crystallized to a sufficiently large size, they are capable of mechanically breaching the protective GAG layer to expose the vulnerable urothelium below. Perhaps the most extreme form of this irritation is seen when stone material becomes impacted in the ureter and can cause intense pain from renal colic and local inflammation. Moreover, impacted ureteral stones produce local fibrosis and are the most common known cause of ureteral strictures that can cause long-term morbidity and need for surgical intervention.1,2 Exactly how these chemical urinary components interface with the urothelium to provoke functional disturbances, fibrosis, and pain is not completely understood.

The consequences of urothelial exposure to noxious chemical stimuli are thought to potentially result from inflammation. Recent studies of inflammation in innate immune cells and epithelial tissues have identified a supramolecular structure, known as the inflammasome, as being critically important in mediating this inflammatory response.3–5 By far the best studied of these is the NLRP3 inflammasome. In fact, our own lab has highlighted the central importance of NLRP3 in triggering inflammation within bladder urothelium in different pathologic states.6–10 The NLRP3 inflammasome is comprised, in part, of the nod-like receptor NLRP3, a structural co-factor protein called thioredoxin-interacting protein (TXNIP), and the adaptor protein ASC. In the presence of activating stimuli, this complex forms, recruits, and activates caspase-1, resulting in the cleavage and maturation of the pro-inflammatory cytokines IL-1β and IL-18. These cytokines are released from the cell via a form of necrotic cell death called pyroptosis, where they go on to promote inflammation.

There are a number of known activators of the inflammasome, including pathogen associated molecular patterns (PAMPs) and damage associated molecular patterns (DAMPs).11 Although many classes of inflammasome can respond to PAMPS, the NLRP3 inflammasome is by far the most well-known to respond to DAMPs.11 In studies of gout and pseudogout,12 calcium pyrophosphate (CPPD) and monosodium urate (MSU), two major components of urinary stones, have been shown to act as DAMPs to stimulate NLRP3 inflammasome activity in macrophages. In similar studies of osteoarthritis, both CPPD and MSU promote inflammation mediated by this inflammasome.13 In their role as DAMPs, these two components are thought to potentiate NLRP3 inflammasome activation by stimulating intracellular reactive oxygen species (ROS) production. Under normal conditions, TXNIP, a structural co-factor of the assembled inflammasome, is bound to the cellular antioxidant thioredoxin. When ROS are produced, they oxidize thioredoxin, resulting in the dissociation of TXNIP. Free TXNIP is then able to bind to NLRP3 to promote formation of the active inflammasome. While ROS-mediated NLRP3 activation, and the importance of TXNIP, have been explored in other cell types,14,15 its role in stone-mediated urothelial inflammation is not yet defined. In this study, we investigated the potential of this pathway in the urothelial response to urinary stone components and identified potential targets to mitigate the subsequent inflammation.

Materials And Methods

Animals

All protocols adhered to the NIH Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use committee at Duke University Medical Center. Female Sprague Dawley rats (≈200 grams, 40–50 days of age, Envigo, Indianapolis, IN) were used in all studies.

Cell Isolation

The cell isolation protocol was modified from a previously published method.6,16 Briefly, rats were sacrificed and the bladders removed and placed in sterile PBS. Bladders were then inverted over an 18-gauge blunt tip needle, inflated with PBS, and a purse string suture was used to tie off the bladder. The inflated bladder was then submerged in Collagenase P (1 mg/mL in complete media) and shaken for 1 hr at 37°C. Cells were then passed through a 40 µm nylon mesh to remove debris, pelleted and resuspended in complete media [F-12K media (HyClone Laboratories, Logan, UT) supplemented with 10% low-endotoxin dialyzed fetal bovine serum (HyClone Laboratories, Logan, UT), 10 µM non-essential amino acids (HyClone Laboratories, Logan, UT), 1.0 µg/mL hydrocortisone (Sigma-Aldrich, St. Louis, MO), 10 µg/mL insulin (Gibco Laboratories, Gaithersburg, Maryland), 5 µg/mL transferrin (Gibco Laboratories, Gaithersburg, MD), 6.7 ng/mL selenium (Gibco Laboratories, Gaithersburg, MD), 100 U/mL penicillin (Gibco Laboratories, Gaithersburg, MD), and 100 µg/mL streptomycin (Gibco Laboratories, Gaithersburg, MD)]. Cells were counted and plated at 50,000 cells/well in 90 µL complete media in black-walled 96-well plates. Cells were then incubated in a water-saturated environment for 24 hrs at 37°C, 95% air, and 5% CO2. Media was removed and replaced with fresh media (90 μL for agonist studies, 80 μL for inhibitor studies) just prior to the start of experimental treatments.

Experimental Treatments

In vitro experiments were performed essentially as previously described.6 For agonist response studies, CPPD and MSU (InvivoGen, San Diego, CA) were prepared to the stock concentrations of 1.25 mg/mL and 12.5 µg/mL, respectively. Using PBS (for CPPD) or complete media (for MSU), 1:2 serial dilutions were prepared. Wells were then treated with 10 µL of the appropriate dilution of stone DAMP for a final volume of 100 µL. After treatment, cells were incubated for another 24 hrs at 37°C.

For inhibition response studies, the general ROS scavenger N-acetylcysteine was prepared to the stock concentration of 5 mM for MSU treatment and to 50 mM for CPPD treatment. Prior to treatment, the stock concentration of NAC was buffered to pH 7.2. Verapamil was prepared to a stock concentration of 1.5 mM for serial dilution and cell treatment. Using complete media for NAC and PBS for Verapamil, 1:2 serial dilutions were prepared. Cells were treated with 10 µL of NAC or Verapamil and incubated at 37°C for 1 or 4 hrs, respectively. After pre-treatment, cells were then treated with 10 µL of CPPD (62.5 µM final) or MSU (1.25 µM final) to a final volume of 100 µL. Plates were then incubated for an additional 24 hrs at 37°C. One hour prior to the end of the incubation, untreated control wells were administered 1.25 mM ATP to serve as a standard for maximal caspase response.

Caspase-1 Assay

The caspase-1 assay was performed as reported.17 Briefly, media were removed and cells lysed in 50 µL lysis buffer (10 mM MgCl2 and 0.25% Igepal CA-630) for 5 mins. An additional 50 µL of storage buffer (40 mM HEPES (pH 7.4), 20 mM NaCl, 2 mM EDTA and 20% glycerol) was added, and the plates frozen at −80°C until used (>30 mins). Plates were then thawed and 50 µL of 50 mM Hepes with 10% Sucrose and 0.1% CHAPS, 10 µL dithiothreitol (final concentration of 5.5 mM), and 20 µL Z-YVAD-AFC substrate (final concentration of 110 µM) were added to each well. Plates were then incubated in the dark for 1 hr at 37°C with mild shaking. Florescence was then measured (excitation 400 nm, emission 505 nm). Florescence in untreated wells (0 mM stone DAMP) was subtracted from all wells and results normalized to the ATP response. Results were reported as a percentage of ATP response.

Statistical Analysis

Statistical analysis was performed by a one-way analysis of variance followed by a Dunnett’s post-hoc analysis using GraphPad InStat software (La Jolla, CA).

Results

CPPD And MSU Produce A Dose-Dependent Increase In Caspase-1 Activation

CPPD triggers a robust and dose-dependent activation of caspase-1 in isolated urothelium in vitro, with a maximal response of approximately 50% of the ATP response and an EC50 of 62.5 μg/mL (Figure 1A). MSU was less efficacious yet more potent than CPPD, with a maximal response of 25% of the ATP response but an EC50 of 0.156 μg/mL (Figure 1B).

Figure 1

The stone DAMPs CPPD and MSU activate caspase-1 in a dose-dependent manner. Urothelial cells were incubated overnight prior to treatment with either CPPD (A) or MSU (B) for 24 hrs. Additional wells were treated with 1.25 mM ATP for 1 hr to indicate maximal caspase-1 activation and DAMP-treated wells were normalized to these ATP-treated wells. n=9 for all doses of CPPD and MSU. *p<0.05; **p<0.01 by one-way ANOVA and Dunnett’s post-hoc test.

N-Acetylcysteine Inhibits Caspase-1 Activation In Cells Treated With Stone DAMPs

To determine if CPPD and MSU signal for inflammasome activation in the urothelia through ROS, we utilized the general ROS scavenger NAC. As shown in Figure 2A, a NAC concentration of 5 mM was sufficient to completely suppress caspase-1 activation in cells treated with CPPD (IC50= 625 μM). However, as shown in Figure 2B, a NAC concentration of just 500 μM was sufficient to completely suppress caspase-1 activation in MSU-treated cells (IC50= 31.25 μM).

Figure 2

NAC inhibits caspase-1 activation in cells treated with CPPD or MSU. Urothelial cells were incubated overnight and then treated with decreasing concentrations of NAC for 1 hr before treatment with 62.5 μg/mL CPPD (A) or 1.25 μg/mL MSU (B) for 24 hrs. The caspase-1 assay was then performed as described in the Materials and Methods section. CPPD-treated cells had a higher IC50 (625 μM) versus MSU-treated cells (IC50= 31.25 μM). n=9 for all NAC treatment doses for both CPPD and MSU. *p<0.05 by one-way ANOVA and Dunnett’s post-hoc test.

Verapamil Inhibits Caspase-1 Activation

Verapamil is a calcium channel blocker that has been shown to downregulate the expression of the NLRP3 binding protein TXNIP,18,19 and thus has been used in several studies to assess a role for this critical structural component of the NLRP3 inflammasome in DAMP pathways mediated by ROS.20,21 As shown in Figure 3A, a dose of 150 μM Verapamil was sufficient to completely inhibit CPPD-mediated caspase-1 activation (IC50=100 μM). In a similar fashion, shown in Figure 3B, MSU-induced caspase-1 activation was also completely suppressed in cells by 150 μM Verapamil (IC50=100 μM).

Figure 3

Verapamil suppresses caspase-1 activation. Urothelial cells were incubated overnight and then treated with decreasing concentrations of Verapamil for 4 hrs before treatment with 62.5 μg/mL CPPD (A) or 1.25 μg/mL MSU (B) for 24 hrs. The caspase-1 assay was then performed as described in the Materials and Methods section. Both CPPD and MSU-treated cells had the same IC50 (100 μM). n= 10 for all doses of Verapamil and CPPD treated wells; n= 5 for all doses of Verapamil and MSU treated wells. *p<0.05 by one-way ANOVA and Dunnett’s post-hoc test.

Discussion

This study provides the first exploration into the urinary DAMP-ROS-NLRP3 inflammasome pathway within the urothelium. We found that NLRP3 is activated in vitro in urothelial cells by two common stone-forming components. Importantly, we also found that it is mediated through an upregulation in intracellular ROS and release of TXNIP from thioredoxin. Specifically, the general ROS scavenger NAC was able to prevent inflammasome activation in both CPPD and MSU-treated urothelial cells. Further, directed targeting of a ROS-responsive protein (TXNIP) that forms a structural component of the NLRP3 inflammasome was also able to prevent inflammasome activity. These findings demonstrate a ROS-driven pathway in stone-induced urothelial inflammation that relies on the TXNIP protein for functionality. Given the consequences of inflammation within the urinary tract, understanding the nuances of this pathway within the urothelium is critical to understanding how to target it clinically in patients.

Interestingly, while NAC and Verapamil both were able to abolish inflammasome activation by CPPD and MSU, we found exciting differences in their respective abilities to do so. First, NAC doses required to completely inhibit inflammasome activation were higher in cells treated with CPPD (5 mM) compared to MSU (500 µM). This differential response is possibly the result of differences in intracellular ROS production by the various stone DAMPs. Second, there were no differences in treatment dose of Verapamil required to completely inhibit inflammasome activation in either CPPD or MSU-treated cells. Maximal inhibition of caspase-1 activity was seen at a Verapamil dose of 150 µM after treatment with either stone DAMP. Therefore, it appears that functional TXNIP is required for the activation of NLRP3 regardless of the magnitude of ROS production. Based on these findings, targeted therapies aimed at impeding different steps within this pathway may be useful to inhibit stone-mediated inflammasome activity.

NAC is an FDA-approved medication that functions as a general ROS scavenger and acts as a precursor molecule for the regeneration of intracellular glutathione, another scavenger of ROS.22 Clinically, NAC is very effective in a number of patient populations, such as acetaminophen overdose, polycystic ovarian syndrome, and chronic bronchitis. While the usefulness of NAC in these patient populations is well-established, its potential in treating urinary DAMP-mediated bladder inflammation has never before been explored. Our findings suggest that this drug may be a useful tool in turning off bladder inflammation caused by urinary DAMPs, thereby allowing for reduction in inflammatory complications.

Verapamil is a non-dihydropyridine calcium channel blocker used in a number of medical conditions, such as hypertension, angina, and cluster headaches. An early study suggested that its calcium channel blocking ability might reduce the level of stone forming components in the urine, but a subsequent study did not corroborate this finding.23,24 However, in this study, verapamil’s ability to downregulate expression of TXNIP25,26 proved a useful tool to block stone-DAMP activation of NLRP3. Therefore, while it may not affect the concentration of stone forming moieties, it may actually protect the urinary tract from these pro-inflammatory urinary components.

This study demonstrates that urinary stone-forming DAMPS activate NLRP3 in urothelial cells via ROS production and require the presence of TXNIP. This pathway is effectively blocked by the administration of the anti-oxidant, NAC, or by down-regulating TXNIP expression with verapamil. These agents may be useful in preventing lower urinary tract inflammation, pain, and risk of fibrosis and scarring in stone forming patients. More broadly, and the subject of future studies, many other urinary DAMPs could also activate the NLRP3 inflammasome and provoke a urothelial inflammatory response via the same pathway demonstrated in this investigation. It is well-known that consumption of certain foods, such as chocolate, alcohol, and acidic juices, can exacerbate lower urinary tract symptoms in sensitive patients. Abstinence from these dietary factors is very effective, but that requires the identification of the specific item which can be challenging. If the pathway elucidated here is common to many other urinary DAMPs, then targeting ROS production, TXNIP expression and/or NLRP3 activation could be implemented as a treatment strategy even if the inciting factor is unknown. Additionally, we have shown in a prior study that diabetic metabolites are capable of activating NLRP3 in urothelium, and this contributes to the development of diabetic bladder dysfunction (DBD).6 If future studies demonstrate that these metabolites have the same mechanism of action as the stone DAMPs (uric acid being an example of both) then targeting ROS production or TXNIP expression may be useful in preventing DBD.

Conclusion

Urinary stones are comprised of components, such as calcium pyrophosphate and monosodium urate, that promote NLRP3 inflammasome activation in vitro within urothelial cells. The present study suggests that urinary stone components drive the activation of the NLRP3 inflammasome by increased intracellular concentrations of ROS that impinge upon TXNIP. Importantly, this study illuminates steps in the pathway that can be pharmacologically impeded through use of N-acetylcysteine and verapamil. Further studies are necessary in vivo to determine the ability of urinary stone components to promote inflammation through NLRP3 and trigger significant inflammatory complications. After such studies, we can then begin to assess the therapeutic potential of these two medications in stone disease.