Qi Ma1, Wei Qian2, Wei Tao2, Yanling Zhou3, Boxin Xue2. 1. Department of Ultrasound, The Second Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. 2. Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. 3. Department of Operation, The Second Affiliated Hospital of Soochow University, Suzhou, People's Republic of China.
Abstract
AIM: The aim of this study was to characterize curcumin (CUR)-loaded CD133 aptamer A15 liposomes for their antitumor activity in vitro and in vivo. METHODS: The modified CUR liposomes were prepared by the thin-film hydration technique. RESULTS: The particles showed spherical shape under electron microscopy with sizes <100 nm. Initial drug burst release was observed within 2 hrs and then the drug was continuously released over 48 hrs. No aggregation or precipitation of liposomes was observed during storage for 3 months. In vitro results showed that blank LPs had lower cellular cytotoxicity. Both liposomes of CUR (with or without A15 modified) exhibited a similar trend of cellular cytotoxicity at the same concentration. With the extension of incubation time, A15-CUR LPs showed a greater inhibitory effect on cells. Cell internalization in DU145 cells was higher for A15-CUR LPs than others. An in vivo study using DU145 prostate carcinoma bearing mice showed that A15-CUR LPs reduced tumor growth more than other forms of CUR. CONCLUSION: These results indicate that A15 modified CUR liposomes are a promising candidate for antitumor drug delivery.
AIM: The aim of this study was to characterize curcumin (CUR)-loaded CD133 aptamer A15 liposomes for their antitumor activity in vitro and in vivo. METHODS: The modified CUR liposomes were prepared by the thin-film hydration technique. RESULTS: The particles showed spherical shape under electron microscopy with sizes <100 nm. Initial drug burst release was observed within 2 hrs and then the drug was continuously released over 48 hrs. No aggregation or precipitation of liposomes was observed during storage for 3 months. In vitro results showed that blank LPs had lower cellular cytotoxicity. Both liposomes of CUR (with or without A15 modified) exhibited a similar trend of cellular cytotoxicity at the same concentration. With the extension of incubation time, A15-CUR LPs showed a greater inhibitory effect on cells. Cell internalization in DU145 cells was higher for A15-CUR LPs than others. An in vivo study using DU145 prostate carcinoma bearing mice showed that A15-CUR LPs reduced tumor growth more than other forms of CUR. CONCLUSION: These results indicate that A15 modified CUR liposomes are a promising candidate for antitumor drug delivery.
Authors: Hsueh-Li Tan; Jennifer M Thomas-Ahner; Elizabeth M Grainger; Lei Wan; David M Francis; Steven J Schwartz; John W Erdman; Steven K Clinton Journal: Cancer Metastasis Rev Date: 2010-09 Impact factor: 9.264