'Artificial peroxidase' nanozyme - enzyme based lactate biosensor.

In contrast to bienzyme biosensors, we propose the nanozyme-enzyme based ones substituting the enzyme peroxidase with the more active and stable nanoparticles "artificial peroxidase". The use of catalytically synthesized Prussian Blue based nanozymes simplifies assembling of hydrogen peroxide transducer providing its higher sensitivity. For immobilization of lactate oxidase the composite alkoxysilane - perfluorosulfonated ionomer (PFSI) membranes are proposed achieving the significantly improved operating stability. The resulting nanozyme-enzyme lactate biosensor displays twice higher sensitivity (>0.2 A M-1 cm-2) compared to the Prussian Blue film based one. Nanozymes "artificial peroxidase" are expected to find wide use in elaboration of oxidase based biosensors.